Development of the self-replicable cell-free protein synthesis system
Project/Area Number |
26710014
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Partial Multi-year Fund |
Research Field |
System genome science
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Shimizu Yoshihiro 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (90401231)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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Keywords | 無細胞タンパク質合成 / リボソーム / tRNA / マイクロ流路 / 生体分子 / 蛋白質 |
Outline of Final Research Achievements |
To achieve the reconstruction of life by a combination of biomolecules, it is considered essential to reconstitute the means for producing proteins themselves in a self-replicable state. In this research, to achieve self-replication of the translation system by itself, we regarded the PURE system, a reconstituted cell-free protein synthesis system, as a fundamental system for the reconstruction of life and developed each technological basis for ribosome reconstitution, tRNA reconstitution, and protein synthesis in a microchannel.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene.2016
Author(s)
Sunagawa GA, Sumiyama K, Ukai-Tadenuma M, Perrin D, Fujishima H, Ukai H, Nishimura O, Shi S, Ohno R, Narumi R, Shimizu Y, Tone D, Ode KL, Kuraku S, Ueda HR.
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Journal Title
Cell Rep.
Volume: 14
Pages: 662-677
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.2014
Author(s)
Susaki, E. A., Tainaka, K., Perrin, D., Kishino, F., Tawara, T., Watanabe, T. M., Yokoyama, C., Onoe, H., Eguchi, M., Yamaguchi, S., Abe, T., Kiyonari, H., Shimizu, Y., Miyawaki, A., Yokota, H., and Ueda, H. R.
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Journal Title
Cell
Volume: 157
Pages: 726-739.
DOI
Related Report
Peer Reviewed
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[Presentation] タンパク質合成の再構成2016
Author(s)
清水義宏
Organizer
日本進化学会第18回大会ワークショップ
Place of Presentation
東京工業大学
Year and Date
2016-08-25 – 2016-08-25
Related Report
Invited
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