| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
Toward cloning of whole genome of a bacterium, Spiroplasma, whole genome of this bacterium was extracted and cut by the rare-cutter restriction enzymes. Then patterns of genome fragments were analyzed by pulse-field gel electrophoresis. These genomic fragments were used for the TAR (Transformation-Associated Recombination)-cloning in yeast cells. Lots of positive clones were obtained, and these clones were checked by several methods. Whole sequences of the insert of several positive clones were analyzed by the Illumina sequencing method. As a result, there was almost no mutation in these inserts, thus this method would be useful for cloning of bacterial genomes.
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