Proton transfer pathways in photosystem II

• Project/Area Number: 26711008
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: Ishikita Hiroshi 東京大学, 先端科学技術研究センター, 教授 (00508111)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000); Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 人工光合成 / プロトン移動 / 光化学系II

Outline of Final Research Achievements

We analyzed the channel, which proceed from the catalytic Mn4CaO5 cluster toward the protein bulk surface of photosystem II (PSII), using the cyanobacterial and plant PSII crystal structures. We also analyzed energetics of the electron transfer pathways in a model protein, because proton transfer is coupled with electron transfer in PSII.

Research Products

• [Journal Article] pKa of ubiquinone, menaquinone, phylloquinone, plastoquinone, and rhodoquinone in aqueous solution (2017)
  • Author(s): Ryo Hasegawa, Keisuke Saito, Tomohiro Takaoka, and Hiroshi Ishikita*
  • Journal Title: Photosynth. Res. Volume: 印刷中 Issue: 1-3 Pages: 297-304
  • DOI: 10.1007/s11120-017-0382-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Electron transfer pathways in a multi-heme cytochrome MtrF (2017)
  • Author(s): Hiroshi C. Watanabe, Yuki Yamashita, and Hiroshi Ishikita*
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. Volume: 114 Issue: 11 Pages: 2916-2921
  • DOI: 10.1073/pnas.1617615114
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structurally conserved channels in cyanobacterial and plant photosystem II (2017)
  • Author(s): Naoki Sakashita, Hiroshi C. Watanabe, Takuya Ikeda, and Hiroshi Ishikita*
  • Journal Title: Photosynth. Res. Volume: 印刷中 Issue: 1-3 Pages: 75-85
  • DOI: 10.1007/s11120-017-0347-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A single amino acid mutation converts (R)-5-diphosphomevalonate decarboxylase into a kinase (2017)
  • Author(s): Kento Motoyama, Hideaki Unno, Ai Hattori, Tomohiro Takaoka, Hiroshi Ishikita, Hiroshi Kawaide, Tohru Yoshimura, and Hisashi Hemmi*
  • Journal Title: J. Biol. Chem. Volume: 292 Issue: 6 Pages: 2457-2469
  • DOI: 10.1074/jbc.m116.752535
  • NAID: 120006305582
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] マルチへムシトクロムMtrFの電子移動経路 (2017)
  • Author(s): 1.渡邉宙志、山下雄己、石北 央
  • Journal Title: 生物物理 Volume: 331
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Energetics of the proton transfer pathway for tyrosine D in photosystem II (2016)
  • Author(s): Keisuke Saito*, Naoki Sakashita, and Hiroshi Ishikita
  • Journal Title: Aust. J. Chem. Volume: 69 Issue: 9 Pages: 991-998
  • DOI: 10.1071/ch16248
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] pKa of a proton conducting water chain in photosystem II (2016)
  • Author(s): Tomohiro Takaoka, Naoki Sakashita, Keisuke Saito, and Hiroshi Ishikita*
  • Journal Title: J. Phys. Chem. Lett. Volume: 7 Issue: 10 Pages: 1925-1932
  • DOI: 10.1021/acs.jpclett.6b00656
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Energetics of proton release on the first oxidation step in the water-oxidizing enzyme (2015)
  • Author(s): K. Saito, A. W. Rutherford, H. Ishikita
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 8488-8488
  • DOI: 10.1038/ncomms9488
  • NAID: 120006348507
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] クロロフィルのかたちは機能にどう影響を及ぼすか: Photosystem IIを例に (2015)
  • Author(s): 斉藤圭亮、石北 央
  • Journal Title: 植物の生長調節 Volume: 50 Pages: 133-138
  • NAID: 110010014906
  • Open Access
• [Presentation] Water-splitting enzyme photosystem II, a model for artificial photosynthesis (2016)
  • Author(s): Hiroshi Ishikita
  • Organizer: UK-Japanese Frontiers of Science Symposium （UK-Japan FoS 日英先端科学シンポジウム)
  • Place of Presentation: Chicheley Hall, UK
  • Year and Date: 2016-11-07
  • Int'l Joint Research / Invited
• [Presentation] Energetics of proton release on the first oxidation step in the water oxidizing enzyme (2016)
  • Author(s): Hiroshi Ishikita
  • Organizer: International Conference on Photochemical Conversion and Storage of Solar Energy
  • Place of Presentation: Saint-Petersburg, Russia
  • Year and Date: 2016-07-24
  • Int'l Joint Research / Invited
• [Presentation] Proton transfer reactions in water oxidizing enzyme Photosystem II (2016)
  • Author(s): Hiroshi Ishikita
  • Organizer: Protein Electrostatics Berlin 2016
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2016-07-19
  • Int'l Joint Research / Invited
• [Presentation] 電子伝達蛋白質がつなぐもの (2016)
  • Author(s): 石北 央
  • Organizer: 生物工学若手研究者の集い（若手会）・夏のセミナー
  • Place of Presentation: ホテルコンチネンタル府中
  • Year and Date: 2016-07-16
  • Invited
• [Presentation] Energetics of proton release on the first oxidation step in the water oxidizing enzyme (2016)
  • Author(s): Hiroshi Ishikita
  • Organizer: International Conference Photosynthesis Research for Sustainability
  • Place of Presentation: Pushchino, Russia
  • Year and Date: 2016-06-19
  • Int'l Joint Research / Invited
• [Presentation] 光合成水分解反応に関わるphotosystem IIの結晶構造から言えるメカニズム (2016)
  • Author(s): 石北 央
  • Organizer: 第1回つくば-柏-本郷イノベーションコリドー・ナノバイオパネル「データ駆動科学、バイオロジーへの挑戦」
  • Place of Presentation: 東京大学 伊藤国際学術研究センター
  • Year and Date: 2016-06-17
  • Invited
• [Presentation] Proton transfer reactions in Photosystem II (2014)
  • Author(s): Hiroshi Ishikita
  • Organizer: 2014 International Conference on Artificial Photosynthesis
  • Place of Presentation: 兵庫県立淡路夢舞台国際会議場（兵庫県、淡路市）
  • Year and Date: 2014-11-24 – 2014-11-28
  • Invited
• [Presentation] Proton transfer reactions and hydrogen bond networks in protein environments (2014)
  • Author(s): Hiroshi Ishikita
  • Organizer: XIXth International Workshop on Quantum Systems, in Chemistry, Physics and Biology
  • Place of Presentation: Fullon Hotel Tamsui Fishermen’s Wharf, Taipei（台湾）
  • Year and Date: 2014-11-11 – 2014-11-17
  • Invited
• [Presentation] 蛋白質環境におけるプロトン移動と水素結合ネットワーク (2014)
  • Author(s): 石北 央
  • Organizer: CBI学会 2014年大会
  • Place of Presentation: タワーホール船堀（千葉県、船橋市）
  • Year and Date: 2014-10-28 – 2014-10-30
  • Invited
• [Presentation] Proton transfer reactions and hydrogen-bond networks in protein environments (2014)
  • Author(s): Hiroshi Ishikita
  • Organizer: 1st UK-Japan bilateral workshop on solar fuel
  • Place of Presentation: 駐日英国大使館（東京都、千代田区）
  • Year and Date: 2014-09-18 – 2014-09-19
  • Invited
• [Presentation] Proton transfer reactions and hydrogen-bond networks in protein environments (2014)
  • Author(s): Hiroshi Ishikita
  • Organizer: Computational Science Workshop 2014
  • Place of Presentation: Tsukuba International Congress Center（茨城県、つくば市）
  • Year and Date: 2014-08-20 – 2014-08-22
  • Invited
• [Presentation] 蛋白質の静電場環境による活性のコントロール (2014)
  • Author(s): 石北 央
  • Organizer: 第14 回日本蛋白質科学会年会ワークショップ
  • Place of Presentation: ワークピア横浜（神奈川県、横浜市）
  • Year and Date: 2014-06-25 – 2014-06-27
  • Invited
• [Presentation] 結晶構造では見えにくい「H+」から蛋白質の反応を見る (2014)
  • Author(s): 石北 央
  • Organizer: 第4期第5回フロンティアサロン
  • Place of Presentation: ホテルニューオータニ東京（東京都、千代田区）
  • Year and Date: 2014-05-19
  • Invited