Probing the epigenetic mechano-chemical regulatory mechanism in the cell lineage by micromechanical manipulation of the early embryo

• Project/Area Number: 26711009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biophysics
• Research Institution: Waseda University
• Principal Investigator: ITABASHI Takeshi 早稲田大学, 理工学術院, 助教 (20434384)
• Research Collaborator: ISHIWATA Shin'ichi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000); Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2014: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 細胞分裂 / 顕微操作

Outline of Final Research Achievements

During cellular development and growth, the mechanical force generated inside and outside a mitotic cell plays a critical role in cellular functions such as changes in cell morphology and the determination of cell division axis. We planned to develop techniques to probe how the cell lineage is affected by the externally applied force to the early embryo. The main results of this project are summarized as follows: (1) Using micromanipulation technique, we found that mechanical properties of spindle poles are symmetrically balanced. (2) The rigidity of surrounding environment affects the process of cell division. (3)We developed the technique to probe the epigenetic mechano-chemical regulatory mechanism in the cell lineage by micromechanical manipulation of the early embryo.

Research Products

• [Journal Article] Dynamic properties of bio-motile systems with a liquid-crystalline structure (2017)
  • Author(s): Shin’ichi Ishiwata, Makito Miyazaki, Katsuhiko Sato, Koutaro Nakagome, Seine A. Shintani, Fuyu Kobirumaki-Shimozawa, Norio Fukuda, Kazuya Suzuki, Jun Takagi, Yuta Shimamoto, Takeshi Itabashi
  • Journal Title: Molecular Crystals and Liquid Crystals Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reproductive strategies in marine hydrozoan jellyfish: sexual medusae and asexual polyps (2017)
  • Author(s): Noriyo Takeda, Ryusaku Deguchi, Takeshi Itabashi
  • Journal Title: Reproductive and Developmental Strategies: the Continuity of Life Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mechanical properties of spindle poles are symmetrically balanced (2017)
  • Author(s): Kazuya Suzuki, Takeshi Itabashi, Shin’ichi Ishiwata
  • Journal Title: Biophysics and Physicobiology Volume: 14 Issue: 0 Pages: 1-11
  • DOI: 10.2142/biophysico.14.0_1
  • NAID: 130005303956
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 顕微力学操作による細胞分裂機構のメカノケミカル制御 (2017)
  • Author(s): 板橋岳志, 石渡信一
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松
  • Year and Date: 2017-03-28
  • Invited
• [Presentation] Dynamic properties of bio-motile systems as a liquid-crystalline structure (2016)
  • Author(s): Shin’ichi Ishiwata, Makito Miyazaki, Katsuhiko Sato, Koutaro Nakagome, Seine A. Shintani, Norio Fukuda, Kazuya Suzuki, Jun Takagi, Yuta Shimamoto, Takeshi Itabashi
  • Organizer: 26th International Liquid Crystal Conference
  • Place of Presentation: Ohio, USA
  • Year and Date: 2016-07-31
  • Int'l Joint Research / Invited
• [Presentation] 顕微力学操作による細胞分裂機能のメカノケミカル制御 (2015)
  • Author(s): 板橋岳志、石渡信一
  • Organizer: 第24回日本バイオイメージング学会学術集会
  • Place of Presentation: 東京理科大学葛飾キャンパス（東京都葛飾区）
  • Year and Date: 2015-09-27
  • Invited
• [Presentation] カンチレバーを用いて細胞分裂機構を探る (2014)
  • Author(s): 板橋岳志、石渡信一
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25 – 2014-09-27
  • Invited
• [Presentation] 紡錘体の力学特性の遷移は染色体分配を手助けする (2014)
  • Author(s): 高木潤、板橋岳志、石渡信一
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25 – 2014-09-27