| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2015: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2014: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
This research project aimed to understand the mechanism of centrosome-independent microtubule generation. The major outcomes are as follows: (1) The augmin complex has been shown required for centrosome-independent microtubule nucleation within the mitotic spindle in animal and plant cells. Here, we identified augmin in the filamentous fungus, elucidating the evolutionary conservation of this protein complex. (2) We generated knockout mice of a critical augmin subunit, and identified a defect in MTOC clustering during early embryonic division. (3) Microtubule nucleation independent of centrosomes or augmin was identified in the moss Physcomitrella patens.
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