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Nuclear dynamics in plants

Research Project

Project/Area Number 26711017
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Shizuoka (2018)
Kyoto University (2014-2017)

Principal Investigator

Tamura Kentaro  静岡県立大学, 食品栄養科学部, 准教授 (40378609)

Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords細胞核 / 核の運動 / シロイヌナズナ / 環境応答 / アクチン繊維 / 定位運動 / ミオシン / 核膜 / イメージング / 花粉管 / ライブイメージング / インタラクトーム / 変異体 / 植物免疫 / サリチル酸 / 核運動 / 核膜孔複合体 / 細胞骨格 / 遺伝学
Outline of Final Research Achievements

Nucleus is most distinctive and important organelles for all eukaryotes. It plays a key role in maintenance and inheritance of genetic materials. In plants, it has been reported that nuclei vary their size and shape during cellular development and move very actively in response to environmental signal. However, little is known about the mechanisms underlying plant nuclear dynamics. In this study, we focused following projects; 1) nuclear movement in response to light, 2) molecular mechanisms underlying formation of nuclear envelope, 3) nuclear pore-mediated defense against pathogens. By combining different approaches including, genetics, imaging, and proteomics, we revealed molecular mechanisms of plant nuclear dynamics. We found that plants have unique systems supporting nuclear dynamics for response to environmental stimuli.

Academic Significance and Societal Importance of the Research Achievements

これまで殆ど共通理解の得られていなかった植物細胞核の形成運動機構について分子レベルでの解析を行った.特に高等植物が独自に持つ高い環境応答能を支える上で重要な分子機構に着目して研究をおこなった.特に,病原体からの防御,紫外線からのDNA保護,そして植物の成長を支えるために必須な核自身の形成機構を明らかにすることができた.これらは植物が固着生活を営む上で独自に手に入れたユニークな生存戦略の一つと考えられる.

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (36 results)

All 2019 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (4 results) Journal Article (22 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 22 results,  Open Access: 18 results,  Acknowledgement Compliant: 6 results) Presentation (10 results) (of which Int'l Joint Research: 6 results,  Invited: 8 results)

  • [Int'l Joint Research] University of Clermont Auvergne/IBMP(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Institut Jean-Pierre Bourgin (IJPB)/IBMP in Strasbourg/University of Clermont Auvergne(France)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Institut Jean-Pierre Bourgin (IJPB)/Clermont University(France)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Clermont Université/IBMP/Institut Jean-Pierre Bourgin(フランス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Comprehensive nuclear proteome of Arabidopsis obtained by sequential extraction2019

    • Author(s)
      Goto Chieko、Hashizume Shoko、Fukao Yoichiro、Hara-Nishimura Ikuko、Tamura Kentaro
    • Journal Title

      Nucleus

      Volume: 10 Issue: 1 Pages: 81-92

    • DOI

      10.1080/19491034.2019.1603093

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Periplasmic Root-Cap Mucilage in Developing Columella Cells of Arabidopsis thaliana2019

    • Author(s)
      Maeda Kazuki、Kunieda Tadashi、Tamura Kentaro、Hatano Kyoko、Hara-Nishimura Ikuko、Shimada Tomoo
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 6 Pages: 1296-1303

    • DOI

      10.1093/pcp/pcz047

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Leaf Endoplasmic Reticulum Bodies Identified in Arabidopsis Rosette Leaves Are Involved in Defense against Herbivory2019

    • Author(s)
      Nakazaki Akiko、Yamada Kenji、Kunieda Tadashi、Sugiyama Ryosuke、Hirai Masami Yokota、Tamura Kentaro、Hara-Nishimura Ikuko、Shimada Tomoo
    • Journal Title

      Plant Physiology

      Volume: 179 Issue: 4 Pages: 1515-1524

    • DOI

      10.1104/pp.18.00984

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Subcellular localisation of an endoplasmic reticulum-plasma membrane tethering factor, SYNAPTOTAGMIN 1, is affected by fluorescent protein fusion2019

    • Author(s)
      K. Ishikawa, Kentaro Tamura, and T. Shimada
    • Journal Title

      Plant Signaling & Behavior

      Volume: 16 Issue: 12 Pages: 1-4

    • DOI

      10.1080/15592324.2018.1547577

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ANGUSTIFOLIA regulate the alignment of actin filaments for homeostatic nuclear positioning in Arabidopsis leaves2019

    • Author(s)
      K. Iwabuchi, H. Ohnishi, Kentaro Tamura, Y. Fukao, H. Tsukaya, and I. Hara-Nishimura
    • Journal Title

      Plant Physiology

      Volume: 179 Issue: 1 Pages: 233-247

    • DOI

      10.1104/pp.18.01150

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Computational methods for studying the plant nucleus2018

    • Author(s)
      A. Poulet, X. Zhou, Kentaro Tamura, I. Meier, C. Tatout, K. Graumann, and D. Evans
    • Journal Title

      Methods in Molecular Biology

      Volume: 1840 Pages: 205-219

    • DOI

      10.1007/978-1-4939-8691-0_15

    • ISBN
      9781493986903, 9781493986910
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synaptotagmin-associated endoplasmic reticulum-plasma membrane contact sites are localized to immobile ER tubules2018

    • Author(s)
      K. Ishikawa, Kentaro Tamura, H. Ueda, Y. Ito, A. Nakano, I. Hara-Nishimura, and T. Shimada
    • Journal Title

      Plant Physiology

      Volume: 178 Issue: 2 Pages: 641-653

    • DOI

      10.1104/pp.18.00498

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The AP-1 complex is required for proper mucilage formation in Arabidopsis seeds2018

    • Author(s)
      T. Shimada, T. Kunieda, S. Sumi, Y. Koumoto, Kentaro Tamura, K. Hatano, H. Ueda, and I. Hara-Nishimura
    • Journal Title

      Plant & Cell Physiology

      Volume: 59 Pages: 2331-2338

    • DOI

      10.1093/pcp/pcy158

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endoplasmic reticulum (ER) membrane proteins (LUNAPARKs) are required for proper configuration of the cortical ER network in plant cells2018

    • Author(s)
      H. Ueda, N. Ohta, Y. Kimori, T. Uchida, T. Shimada, Kentaro Tamura, and I. Hara-Nishimura
    • Journal Title

      Plant & Cell Physiology

      Volume: 59 Issue: 10 Pages: 1931-1941

    • DOI

      10.1093/pcp/pcy137

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana2017

    • Author(s)
      Kentaro Tamura, Y. Fukao, N. Hatsugai, F. Katagiri, I. Hara-Nishimura
    • Journal Title

      Nucleus

      Volume: 印刷中 Issue: 3 Pages: 301-311

    • DOI

      10.1080/19491034.2017.1279774

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Exploring the protein composition of the plant nuclear envelope2017

    • Author(s)
      X. Zhou, Kentaro Tamura, K. Graumann, and I. Meier
    • Journal Title

      Methods Mol Biol.

      Volume: 1411 Pages: 45-66

    • DOI

      10.1007/978-1-4939-3530-7_2

    • ISBN
      9781493935284, 9781493935307
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Decreased expression of a gene caused by a T-DNA insertion in an adjacent gene in Arabidopsis2016

    • Author(s)
      K. Tamura, T. Kawabayashi, T. Shikanai, and I. Hara-Nishimura
    • Journal Title

      PLoS One

      Volume: 11 Issue: 2 Pages: e0147911-e0147911

    • DOI

      10.1371/journal.pone.0147911

    • NAID

      120005743647

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plant nuclei move to escape ultraviolet-induced DNA damage and cell death.2016

    • Author(s)
      Iwabuchi K., Hidema J., Tamura K., Takagi S., Hara-Nishimura I.
    • Journal Title

      Plant Physiology

      Volume: 170 Issue: 2 Pages: 678-685

    • DOI

      10.1104/pp.15.01400

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis.2016

    • Author(s)
      Ueda, H., Yokota, E., Kuwata, K., Kutsuna, N., Mano, S., Shimada, T., Tamura, K., Stefano, G., Fukao, Y., Brandizzi, F., Shimmen, T., Nishimura, M., Hara-Nishimura, I.
    • Journal Title

      Plant Physiol.

      Volume: 170 Issue: 2 Pages: 867-880

    • DOI

      10.1104/pp.15.01172

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Functions of plant-specific myosin XI: from intracellular motility to plant postures.2015

    • Author(s)
      Ueda, H., Tamura, K., Hara-Nishimura, I.
    • Journal Title

      Curr. Opin. Plant Biol.

      Volume: 28 Pages: 30-38

    • DOI

      10.1016/j.pbi.2015.08.006

    • NAID

      120005676685

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Recent advances in understanding plant nuclear envelope proteins involved in nuclear morphology2015

    • Author(s)
      Kentaro Tamura, Chieko Goto and Ikuko Hara-Nishimura
    • Journal Title

      Journal of Experimental Botany

      Volume: 66 Issue: 6 Pages: 1641-1647

    • DOI

      10.1093/jxb/erv036

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation of organ straightening and plant posture by an actin-myosin XI cytoskeleton2015

    • Author(s)
      Okamoto, K., Ueda, H., Shimada, T., Tamura, K., Kato, T., Tasaka, M., Morita, M.T., and Hara-Nishimura, I.
    • Journal Title

      Nature Plants

      Volume: 1 Issue: 4 Pages: 1-6

    • DOI

      10.1038/nplants.2015.31

    • NAID

      120005593639

    • Description
      【掲載確定】
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BEACH-Domain Proteins Act Together in a Cascade to Mediate Vacuolar Protein Trafficking and Disease Resistance in Arabidopsis.2015

    • Author(s)
      Teh OK, Hatsugai N, Tamura K, Fuji K, Tabata R, Yamaguchi K, Shingenobu S, Yamada M, Hasebe M, Sawa S, Shimada T, Hara-Nishimura I.
    • Journal Title

      Mol Plant

      Volume: 8 Issue: 3 Pages: 389-398

    • DOI

      10.1016/j.molp.2014.11.015

    • NAID

      120005646465

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] GFS9/TT9 contributes to intracellular membrane trafficking and flavonoid accumulation in Arabidopsis thaliana2014

    • Author(s)
      Ichino T, Fuji K, Ueda H, Takahashi H, Koumoto Y, Takagi J, Tamura K, Sasaki R, Aoki K, Shimada T, Hara-Nishimura I.
    • Journal Title

      Plant J

      Volume: 80 Issue: 3 Pages: 410-423

    • DOI

      10.1111/tpj.12637

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nucleoporin 75, a component of nuclear pore complex, is involved in the MAPK-mediated production of ethylene leading to the accumulation of phytoalexin for the resistance of Nicotiana benthamiana to Phytophthora infestans2014

    • Author(s)
      Ohtsu et al.
    • Journal Title

      Mol. Plant Microbe Interact.

      Volume: 27 Issue: 12 Pages: 1318-1330

    • DOI

      10.1094/mpmi-06-14-0181-r

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional insights of nucleocytoplasmic transport in plants2014

    • Author(s)
      Tamura K, Hara-Nishimura I
    • Journal Title

      Front Plant Sci

      Volume: 5 Pages: 1-10

    • DOI

      10.3389/fpls.2014.00118

    • NAID

      120005439543

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The novel nuclear envelope protein KAKU4 modulates nuclear morphology in Arabidopsis2014

    • Author(s)
      C. Goto, K. Tamura, Y. Fukao, T. Shimada, and I. Hara-Nishimura
    • Journal Title

      Plant Cell

      Volume: 25 Pages: 4658-4675

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Nucleocytoplasmic transport functions in environmental responses of higher plants2018

    • Author(s)
      Kentaro Tamura
    • Organizer
      Annual Meeting of Molecular Biology Society of Japan
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Dynamic regulation of plant nuclear architecture2018

    • Author(s)
      Kentaro Tamura
    • Organizer
      Annual Meeting of the Botanical Society of Japan
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Diverse functions of plant nuclear envelope proteins2018

    • Author(s)
      Kentaro Tamura
    • Organizer
      Society for Experimental Biology Annual Main Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nuclear movement and shape are controlled by nuclear membrane myosin XI-i2016

    • Author(s)
      Kentaro Tamura and Ikuko Hara-Nishimura
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岩手大学,盛岡市,岩手県
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Emerging roles for nuclear membrane in signalling pathway in higher plants.2016

    • Author(s)
      Kentaro Tamura
    • Organizer
      Society for Experimental Biology Annual Main Meeting
    • Place of Presentation
      Brighton, United Kingdom
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The unique nucleocytoplasmic linkage in plants2015

    • Author(s)
      Kentaro Tamura
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートピアランド,神戸市,兵庫県
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular relationships between the plant-specific nuclear envelope proteins2015

    • Author(s)
      Kentaro Tamura
    • Organizer
      IPNC2015
    • Place of Presentation
      Olomouc, Czech Republic
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Interactive proteomics dissects structural dynamics of plant nuclear envelope2014

    • Author(s)
      Kentaro Tamura, Yoichiro Fukao, and Ikuko Hara-Nishimura
    • Organizer
      1st INPPO World Congress
    • Place of Presentation
      University of Hamburg, Hamburg, Germany
    • Year and Date
      2014-09-01
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Key regulators of nuclear dynamics in Arabidopsis2014

    • Author(s)
      Kentaro Tamura
    • Organizer
      SEB2014
    • Place of Presentation
      Manchester University, Manchester, United Kingdom
    • Year and Date
      2014-07-02
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Myosin XI-i is required for nuclear movement and shape in Arabidopsis2014

    • Author(s)
      Kentaro Tamura
    • Organizer
      ECAB2014
    • Place of Presentation
      Postech Biotech Centre, Pohang, Korea
    • Year and Date
      2014-04-03
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research

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Published: 2014-04-04   Modified: 2020-03-30  

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