Elucidation of abscisic acid signaling pathway in plant growth by chemical genetic analysis

• Project/Area Number: 26711018
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tottori University
• Principal Investigator: Okamoto Masanori 鳥取大学, 乾燥地研究センター, 助教 (50455333)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000); Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: Abscisic acid / Chemical biology / Molecular physiology / Plant hormones / アブシジン酸 / 植物ホルモン / ケミカルバイオロジー / 遺伝学 / シグナル伝達 / 成長制御 / 植物生理学 / 分子生物学 / 受容体 / 環境応答 / ストレス適応

Outline of Final Research Achievements

Physiological function of monomeric abscisic acid (ABA) receptors among ABA receptor has not been revealed. We have developed ABA sensor using yeast two-hybrid system, and it’s sensor was used for finding selective ABA agonist to monomeric receptor. As the result, we found 115C07 compound, which inhibited only hypocotyl growth in the dark condition. Chemical genetic screens with 115C07 have identified PYL8, ABI3, ABI4, RNA processing and kinase component mutants, suggesting that these components are involved in tissue specific plant growth.

Research Products

• [Journal Article] アブシジン酸受容体と受容体に作用する化合物について (2017)
  • Author(s): 岡本 昌憲
  • Journal Title: 植調 Volume: 50 Pages: 2-7
• [Journal Article] Chemical Promotion of Endogenous Amount of ABA in Arabidopsis thaliana by a Natural Product, Theobroxide. (2016)
  • Author(s): Yamashita, Y., Ota, M., Inoue, Y., Hasebe, Y., Okamoto, M., Inukai, T., Masuta, C., Sakihama, Y., Hasidoko, Y., Kojima, M., Sakakibara, H., Inage, Y., Takahashi, K., Yoshihara, T., and Matsuura H.
  • Journal Title: Plant Cell and Physiology Volume: 57 Issue: 5 Pages: 986-999
  • DOI: 10.1093/pcp/pcw037
  • Peer Reviewed
• [Journal Article] Sm-like protein-mediated RNA metabolism is required for heat stress tolerance in Arabidopsis. (2016)
  • Author(s): Okamoto, M., Matsui, A., Tanaka, M., Morosawa, T., Ishida, J., Iida, K., Mochizuki, Y., Toyoda, T. and Seki, M.
  • Journal Title: Front. Plant Sci. Volume: 7 Pages: 1079-1079
  • DOI: 10.3389/fpls.2016.01079
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis. (2016)
  • Author(s): Yan D. Easwaran V. Chau V. Okamoto M. Ierullo M. Kimura M. Endo A. Yano R. Pasha A. Gong Y. Bi YM. Provart N. Guttman D. Krapp A. Rothstein SJ. Nambara E.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 13179-13179
  • DOI: 10.1038/ncomms13179
  • NAID: 120007129295
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] a practical inhibitor of abscisic acid 8’-hydroxylase for improving drought tolerance. (2016)
  • Author(s): Takeuchi J*. Okamoto M*, Mega R. Kanno Y. Ohnishi T. Seo M. Todoroki Y. Abscinazole-E3M, a practical inhibitor of abscisic acid 8’-hydroxylase for improving drought tolerance.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 37060-37060
  • DOI: 10.1038/srep37060
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] アブシジン酸の代謝と受容に関する化学遺伝学的研究 (2016)
  • Author(s): 岡本 昌憲
  • Journal Title: 植物の生長調節 Volume: 51 Pages: 16-23
  • NAID: 130005394579
• [Journal Article] Conformationally restricted 3’-modi␣ed ABA analogs for controlling ABA receptors (2015)
  • Author(s): Takeuchi J. Ohnishi T. Okamoto M. Todoroki Y.
  • Journal Title: Organic & Biomolecular Chemistry Volume: 13 Issue: 14 Pages: 4278-4288
  • DOI: 10.1039/c4ob02662d
  • Peer Reviewed
• [Journal Article] The selectivity of 6-nor-ABA and 7'-nor-ABA for abscisic acid receptor subtypes. (2015)
  • Author(s): Takeuchi J. Ohnishi T. Okamoto M. Todoroki Y.
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 25 Issue: 17 Pages: 3507-10
  • DOI: 10.1016/j.bmcl.2015.06.088
  • NAID: 120006319284
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Designed abscisic acid analogues as antagonists of PYL-PP2C receptor interactions (2014)
  • Author(s): Takeuchi J.* Okamoto M.* Akiyama T. Muto T. Yamaji S. Sue M. Seo M. Kanno Y. Kamo T. Endo A. Nambara E. Hirai N. Ohnishi T. Cutler SR. Todoroki Y.
  • Journal Title: Nature Chemical Biology Volume: 10 Issue: 6 Pages: 477-482
  • DOI: 10.1038/nchembio.1524
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Enhancement of abscisic acid receptor improves water use efficiency in crop production (2017)
  • Author(s): Okamoto M.
  • Organizer: International Symposium on Environmental Stress Adaptation and Memory in Plants
  • Place of Presentation: Yokohama, Kanagawa
  • Year and Date: 2017-02-27
  • Int'l Joint Research / Invited
• [Presentation] 植物の乾燥ストレス応答を制御するアブシジン酸の働きとその応用 (2017)
  • Author(s): 岡本昌憲
  • Organizer: 第18回 静岡ライフサイエンスシンポジウム
  • Place of Presentation: 静岡県, 静岡市
  • Invited
• [Presentation] Enhancement of ABA receptor confers water-saving drought tolerance in wheat (2016)
  • Author(s): Okamoto M. Abe F. Kim JS. Tanaka K. Kobayashi H. Sakata Y. Hanada K. Mega R.
  • Organizer: 22nd International Plant Growth Regulators Association
  • Place of Presentation: Toront, Canada
  • Year and Date: 2016-06-21
  • Int'l Joint Research
• [Presentation] ABA受容体を利用したコムギの乾燥ストレス耐性の向上 (2016)
  • Author(s): 岡本昌憲、安倍史高、金俊植、辻本壽、花田耕介、妻鹿良亮
  • Organizer: 第57回 日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] アブシジン酸受容体に作用する人工化合物の開発とその利用 (2016)
  • Author(s): 岡本昌憲
  • Organizer: 第8回 植物ストレス科学研究シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2016-03-07
  • Invited
• [Presentation] アブシジン酸の代謝と受容に関する化学遺伝学的研究 (2015)
  • Author(s): 岡本昌憲
  • Organizer: 植物化学調節学会 第50回記念大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-10-28
  • Invited
• [Presentation] 植物の乾燥ストレス応答を制御するアブシジン酸の働きとその応用 (2015)
  • Author(s): 岡本昌憲
  • Organizer: 第16回 極限環境生物学会シンポジウム
  • Place of Presentation: 京都大学
  • Year and Date: 2015-06-06
  • Invited
• [Presentation] 植物ホルモンのアブシジン酸受容体に作用する化合物開発 (2015)
  • Author(s): 岡本昌憲
  • Organizer: 第7回農芸化学の未来開拓セミナー
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-05-15
  • Invited
• [Presentation] アブシジン酸シグナル伝達を制御する低分子化合物の同定と利用 (2015)
  • Author(s): 岡本昌憲
  • Organizer: 第56回日本植物生理学会
  • Place of Presentation: 東京農業大学 世田谷キャンパス
  • Year and Date: 2015-03-16
  • Invited
• [Presentation] 乾燥ストレス応答におけるアントシアニン量の調節 (2014)
  • Author(s): 岡本昌憲
  • Organizer: 第6回中国地域育種談話会
  • Place of Presentation: 鳥取大学
  • Year and Date: 2014-12-20
• [Presentation] 乾燥ストレス応答におけるアントシアニン量の調節 (2014)
  • Author(s): 岡本昌憲、田中真帆、諸澤妙子、南原英司、関原明
  • Organizer: 第49回植物化学調節学会
  • Place of Presentation: 京都大学
  • Year and Date: 2014-10-17
• [Presentation] ゲノム解析によるコムギABA受容体の同定および生化学的解析 (2014)
  • Author(s): 妻鹿良亮、花田耕介、岡本昌憲
  • Organizer: 第49回植物化学調節学会
  • Place of Presentation: 京都大学
  • Year and Date: 2014-10-17
• [Presentation] Chemical genetic dissection for abscisic acid receptor function by using a synthetic ABA agonist (2014)
  • Author(s): Masanori Okamoto, Francis C. Peterson and Sean R. Cutler
  • Organizer: THE 38th NAITO CONFERENCE, Molecule-based biological systems
  • Place of Presentation: Sapporo
  • Year and Date: 2014-09-07
• [Book] Physiological action and application of abscisic acid, which controls drought stress response in plants (2015)
  • Author(s): Okamoto M and Mega R
  • Publisher: 極限環境生物学会誌
• [Book] Abscisic acid: Metabolism, Transport and Signaling (2014)
  • Author(s): Akira Endo, Masanori Okamoto, Tomokazu Koshiba
  • Total Pages: 465
  • Publisher: Springer
• [Remarks] 鳥取大学 乾燥地研究センター 植物分子生物学研究室
  • URL: http://www.alrc.tottori-u.ac.jp/staff204/okamoto/TOP.html
• [Remarks] 植物の乾燥耐性能を向上させる化合物の開発に成功
  • URL: http://www.alrc.tottori-u.ac.jp/news16/pressrelease-okamoto.pdf
• [Remarks] 鳥取大学乾燥地研究センター植物分子生物学研究室
  • URL: http://www.alrc.tottori-u.ac.jp/staff204/okamoto/TOP.html