Elucidation of a primary step of blue light signaling in guard cells
| Project/Area Number |
26711019
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Yamaguchi University (2016-2018)
Kyushu University (2014-2015) |
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Principal Investigator |
Takemiya Atsushi 山口大学, 大学院創成科学研究科, 准教授 (80448406)
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| Project Period (FY) |
2014-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 青色光 / フォトトロピン / プロテオーム / リン酸化 / 気孔 / シグナル伝達 / 植物 / 遺伝学 |
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| Outline of Final Research Achievements |
Stomata open in response to blue light, allowing CO2 uptake for photosynthetic carbon fixation and nutrient uptake via transpiration. Blue light perception by phototropins, light-activated receptor kinases, mediates the activation of plasma membrane H+-ATPase, which creates a driving force for stomatal opening. Recently, we have identified a novel protein kinase BLUS1 as a substrate of phototropin kinases in guard cells. In this study, we succeeded in reconstituting a primary step of blue light signaling in vitro using recombinant proteins of phototropins and BLUS1. We further identified a CBC1 kinase as another substrate of phototropin kinases involved in different regulatory mechanisms of stomatal opening.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では青色光シグナル伝達の初期過程を試験管内で再現することに成功した。本研究で確立したアッセイ系は、生命の光情報の変換過程を分子・構造レベルで解明するための有用なツールとなることが期待される。また、本研究ではタンパク質のリン酸化の網羅的な解析により、フォトトロピンの新奇リン酸化基質を同定することに成功し、気孔開口の新たなモデルが見えてきた。今後は上記の解析で見出された他の因子の解析を進めることで、フォトトロピンが制御する光シグナル伝達ネットワークの全貌が明らかになることが期待される。
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Report
(6 results)
Research Products
(36 results)
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[Journal Article] Brassinosteroid involvement in Arabidopsis thaliana stomatal opening.2017
Author(s)
Inoue S, Iwashita N, Takahashi Y, Gotoh E, Okuma E, Hayashi M, Tabata R, Takemiya A, Doi M, Murata Y, Kinoshita T. and Shimazaki K.
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Journal Title
Plant & Cell Physiol.
Volume: 58
Issue: 6
Pages: 1048-1058
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] RPT2/NCH1 subfamily of NPH3-like proteins is essential for the chloroplast accumulation response in land plants2016
Author(s)
Suetsugu, N., Takemiya, A., Kong, S.-G., Higa, T., Komatsu, A., Shimazaki, K. Kohchi, T. and Wada, M.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 113
Issue: 37
Pages: 10424-10429
DOI
NAID
Related Report
Peer Reviewed
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