| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Stomata open in response to blue light, allowing CO2 uptake for photosynthetic carbon fixation and nutrient uptake via transpiration. Blue light perception by phototropins, light-activated receptor kinases, mediates the activation of plasma membrane H+-ATPase, which creates a driving force for stomatal opening. Recently, we have identified a novel protein kinase BLUS1 as a substrate of phototropin kinases in guard cells. In this study, we succeeded in reconstituting a primary step of blue light signaling in vitro using recombinant proteins of phototropins and BLUS1. We further identified a CBC1 kinase as another substrate of phototropin kinases involved in different regulatory mechanisms of stomatal opening.
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