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The analysis for the regulation of chromosome segregation during meiosis I

Research Project

Project/Area Number 26711020
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Genetics/Chromosome dynamics
Research InstitutionThe University of Tokyo

Principal Investigator

Sakuno Takeshi  東京大学, 分子細胞生物学研究所, 准教授 (10504566)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords減数分裂 / 染色体分配 / コヒーシン / MEIKIN / 相同組換え / CK1 / 一方向性結合 / Polo / 染色体動態 / 分裂酵母 / 組換え / シュゴシン / コヒーシン複合体 / 還元分配 / カゼインキナーゼ
Outline of Final Research Achievements

Throughout this study, we found that the mechanism necessary for sister kinetochores to be captured mono-oriented manner by spindle microtubules during meiosis I is controlled through MEIKIN, which is functionally conserved from yeasts to mouse. We also showed that yeast and murine Polo-like kinase is required for the regulation of the localization of Shugosin, which is essential for the protection of cohesin from cleavage after meiosis I. Furthermore, it was found that phosphorylation of cohesin by casein kinase I is essential for the regulation of meiotic recombination necessary for the reductional segregation of homologous chromosomes. From these results, the understanding of the molecular mechanism responsible for the regulation of chromosome segregation during meiosis I was largely progressed.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Hierarchical regulation of centromeric cohesion protection by meikin and shugoshin during meiosis I2017

    • Author(s)
      Miyazaki S, Kim J, Sakuno T and Watanabe Y.
    • Journal Title

      Cold Spring Harbor Symp. on Quantitative Chromosome Segregation & Structure

      Volume: 82 Pages: 811-827

    • DOI

      10.1101/sqb.2017.82.033811

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I2017

    • Author(s)
      Seira Miyazaki, Jihye Kim, Yuya Yamagishi, Tadashi Ishiguro, Yuki Okada, Yuji Tanno, Takeshi Sakuno and Yoshinori Watanabe
    • Journal Title

      Genes to Cells

      Volume: 印刷中 Issue: 6 Pages: 552-567

    • DOI

      10.1111/gtc.12496

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analysis of Schizosaccharomyces pombe meiosis.2017

    • Author(s)
      Yamashita A, Sakuno T, Watanabe Y and Yamamoto M.
    • Journal Title

      Spring Harbor Protocols

      Volume: 11 Issue: 9 Pages: 251-270

    • DOI

      10.1101/pdb.top079855

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Meikin is a conserved regulator of meiosis-I-specific kinetochore function2015

    • Author(s)
      Kim, J., Ishiguro, K., Nambu, A., Akiyoshi, B., Yokobayashi, S., Kagami, A., Ishiguro, T., Pendas, A.M., Takeda, N., Sakakibara, Y., Kitajima, T.S., Tanno, Y., Sakuno, T., and Watanabe, Y.
    • Journal Title

      Nature

      Volume: 517 Issue: 7535 Pages: 466-471

    • DOI

      10.1038/nature14097

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of Cohesin Recll/SA3 by Casein Kinase 1 Promotes Homologous Recombination by Assembling the Meiotic Chromosome Axis2015

    • Author(s)
      Sakuno, T., and Watanabe, Y.
    • Journal Title

      Developmental Cell

      Volume: 32 Issue: 2 Pages: 220-230

    • DOI

      10.1016/j.devcel.2014.11.033

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] コヒーシンを介した減数分裂期における染色体の高次構造形成機構2017

    • Author(s)
      作野剛士
    • Organizer
      酵母遺伝学フォーラム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cohesin-dependent higher order chromosomal structure is required for meiotic recombination2017

    • Author(s)
      Takeshi Sakuno
    • Organizer
      SMC meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] コヒーシンを介した減数分裂期における染色体の高次構造形成機構2016

    • Author(s)
      作野 剛士
    • Organizer
      酵母遺伝学フォーラム年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular mechanism of cohesin-mediated chromosome axis assembly in meiosis2015

    • Author(s)
      Takeshi Sakuno and Yoshinori Watanabe
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      広島大学 (広島県東広島市)
    • Year and Date
      2015-08-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular mechanism of cohesin-mediated chromosome axis assembly in meiosis2015

    • Author(s)
      Takeshi Sakuno and Yoshinori Watanabe
    • Organizer
      分裂酵母国際会議
    • Place of Presentation
      生田神社会館 (兵庫県神戸市)
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Phosphorylation of meiotic cohesin by Casein kinase 1 promotes homologous recombination by assembling the chromosome axis structure2014

    • Author(s)
      Takeshi Sakuno and Yoshinori Watanabe
    • Organizer
      The 9th International 3R symposium
    • Place of Presentation
      Gotenba, Shizuoka, Japan
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Book] 細胞工学2015

    • Author(s)
      澁谷大輝 作野剛士
    • Total Pages
      86
    • Publisher
      秀潤社
    • Related Report
      2015 Annual Research Report
  • [Remarks] 生殖細胞での遺伝子組換えのメカニズムの一端を解明

    • URL

      http://www.iam.u-tokyo.ac.jp/pressrelease150126.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 生殖細胞の染色体分配様式をつくりだす要のタンパク質を発見

    • URL

      http://www.iam.u-tokyo.ac.jp/pressrelease141225.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] CK1は減数分裂に特異的なコヒーシンのリン酸化を介して染色体の軸構造の形成と組換えを制御する

    • URL

      http://first.lifesciencedb.jp/archives/9770

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京大学分子細胞生物学研究所プレスリリース

    • URL

      http://www.iam.u-tokyo.ac.jp/pressrelease150126.html

    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2019-03-29  

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