Genome-wide association study of agronomic traits in the rice nested association mapping population

• Project/Area Number: 26712002
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: Akira Abe 公益財団法人岩手生物工学研究センター, ゲノム育種研究部, 主任研究員 (80503606)
• Research Collaborator: TAKAGI Hiroki ; UTSUSHI Hiroe ; YAEGASHI Hiroki
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000); Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2014: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: イネ / GWAS / NAM / 多収性 / 葉身形態 / QTL / 育種学 / 遺伝学 / アソシエーション解析 / ゲノム

Outline of Final Research Achievements

We have developed the rice nested association mapping (NAM) population, composed of 3,000 RILs derived from the crosses of a common parent ‘Hitomebore’ with each of 20 diverse vrieties. These RILs of F7-F9 generations were genotyped using high density SNPs that were detected by whole genome re-sequencing. Through a genome-wide association study (GWAS) of the rice NAM population, we determined the genetic basis of important agronomic traits and identified some of the QTLs.
These results demonstrate that the use of GWAS with NAM populations is effective in uncovering the basis of important agronomic traits.

Research Products

• [Journal Article] Rice breeding based on whole genome sequencing using next-generation sequencer (2016)
  • Author(s): Akira Abe, Hiroki Takagi, Satoshi Natsume, Hiroki Yaegashi, Hideko Kikuchi, Kentaro Yoshida, Shunichi Kosugi, Hiromasa Saitoh, Naoya Urasaki, Hideo Matsumura, Hiroyuki Kanzaki, and Ryohei Terauchi
  • Journal Title: 生化学 Volume: 88 Issue: 1 Pages: 44-53
  • DOI: 10.14952/SEIKAGAKU.2016.880044
  • ISSN: 0037-1017
  • Year and Date: 2016-02-25
  • Peer Reviewed / Open Access
• [Journal Article] Potato research achievement and challenges (2015)
  • Author(s): Utsushi, H., Abe, A., Tamiru, M., Ogasawara, Y., Obara, T., Sato, E., Ochiai, Y., Terauchi, R. and Takagi, H.
  • Journal Title: Breeding Science Volume: 65 Issue: 1 Pages: 1-1
  • DOI: 10.1270/jsbbs.65.1
  • NAID: 130005066138
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] イネ Nested Association Mapping 集団による農業形質の遺伝解析 (2016)
  • Author(s): 阿部陽, 高木宏樹, 八重樫弘樹, 植村亜衣子, 及川香梨, 宇津志博恵, 菊池秀子, 小笠原由美子, 清水元樹, 寺内良平
  • Organizer: 日本育種学会第130回講演会
  • Place of Presentation: 鳥取大学
  • Year and Date: 2016-10-07
• [Presentation] イネ組換え近交系200系統の全ゲノムシーケンス情報を用いた高密度ジェノタイピング (2015)
  • Author(s): 及川香梨、植村亜衣子、八重樫弘樹、菊池秀子、阿部陽、寺内良平、高木宏樹
  • Organizer: 日本育種学会
  • Place of Presentation: 新潟大学
  • Year and Date: 2015-09-11
• [Presentation] 岩手県における水稲ゲノム育種の取り組み (2014)
  • Author(s): 阿部陽
  • Organizer: 第9回東北育種研究集会
  • Place of Presentation: 岩手大学
  • Year and Date: 2014-11-15
  • Invited