Reconstruction of an evolution process with allopolyploidization based on whole genome seqeunce information

• Project/Area Number: 26712003
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Crop production science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Mochida Keiichi 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (90387960)
• Research Collaborator: Sakurai Tetsuya 国立研究開発法人理化学研究所, 環境資源科学研究センター, ユニットリーダー (90415167) ; Yamaguchi Yukiko (Uehara Yukiko) 国立研究開発法人理化学研究所, 環境資源科学研究センター, テクニカルスタッフ
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000); Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: コムギ / 異質倍数性 / トランスクリプトーム / 同祖遺伝子 / 倍数性進化 / 農林水産物 / ゲノム

Outline of Final Research Achievements

To investigate differentiations of gene expression of homoeologs located on A, B and D- genome in common wheat, we constructed and analyzed a co-expression gene network of wheat homoeologs based on more than 700 RNA-seq data publicly available in wheat. Moreover, we performed transcriptome analysis of various wheat related species including A-genome ancestors, tetraploid wheat and hexaploid wheat to investigate functional differentiation of homoeologs through alloployploidization. Then, we revealed a putative relation between transcriptional changes in response to temperature environments and genome polyploidization of wheat.

Research Products

• [Journal Article] Exploring Genetic Diversity in Plants Using High-Throughput Sequencing Techniques (2016)
  • Author(s): Onda Y, Mochida K
  • Journal Title: Current Genomics Volume: 17 Issue: 4 Pages: 356365-356365
  • DOI: 10.2174/1389202917666160331202742
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops. (2015)
  • Author(s): Onda, Y., Hashimoto, K., Yoshida, T., Sakurai, T., Sawada, Y., Hirai, M.Y., Toyooka, K., Mochida, K. and Shinozaki, K.
  • Journal Title: Proceedings of the Royal Society B Volume: 282 Issue: 1811 Pages: 1-8
  • DOI: 10.1098/rspb.2015.0964
  • Peer Reviewed / Open Access
• [Journal Article] Crop improvement using life cycle datasets acquired under field conditions (2015)
  • Author(s): Mochida K, Saisho D, Hirayama T.
  • Journal Title: Front. Plant Sci. Volume: 6 Pages: 740-740
  • DOI: 10.3389/fpls.2015.00740
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Crop design based on life cycle datasets toward sustainable agriculture (2017)
  • Author(s): Keiichi Mochida
  • Organizer: 第9回植物ストレス科学研究シンポジウム
  • Place of Presentation: 岡山県倉敷市
  • Int'l Joint Research / Invited
• [Presentation] 異質倍数体植物の環境適応性に関わるゲノム機能の研究 (2016)
  • Author(s): 高萩航太郎、井上小槙、中山梨沙、清水みなみ、山口(上原)由紀子、恩田義彦、篠崎一雄、持田恵一
  • Organizer: 第11回ムギ類研究会
  • Place of Presentation: 岡山県倉敷市
  • Invited