| Budget Amount *help |
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
In plants, it is difficult to identify genes essential for development and genes having redundant functions and so on through current forward genetic screenings, for example EMS and T-DNA mutagenesis. In this study, to overcome these limitations, I established a novel forward genetic screening system in plants, in which I utilized an endogenous small RNA pathway called TAS1 tasiRNA. Then, by using the established system, I tried to identify novel host factors of a plant virus, Cucumber mosaic virus (CMV). Repression of TOM1 and TOM3 genes by synthetic artificial tasiRNAs in Arabidopsis reproduced the Tobacco mosaic virus-resistant phenotype of tom1/tom3 double mutant, strongly suggesting that we can identify host factors of plant viruses by using the established forward genetic screening system. I then set up the screening to identify host factors, which localize on vacuole membrane or are involved in phospholipid metabolism, required for CMV replication.
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