Development of a novel forward genetic screening system by using small RNA in plants
| Project/Area Number |
26712006
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
Takeda Atsushi 立命館大学, 生命科学部, 准教授 (60560779)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 植物ー病原体相互作用 / RNAiスクリーニング / バイオテクノロジー / 遺伝学 / 植物ウイルス / 植物-病原体相互作用 / 植物－病原体相互作用 / 順遺伝学スクリーニング / RNAサイレンシング / 宿主因子 / tasiRNA |
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| Outline of Final Research Achievements |
In plants, it is difficult to identify genes essential for development and genes having redundant functions and so on through current forward genetic screenings, for example EMS and T-DNA mutagenesis. In this study, to overcome these limitations, I established a novel forward genetic screening system in plants, in which I utilized an endogenous small RNA pathway called TAS1 tasiRNA. Then, by using the established system, I tried to identify novel host factors of a plant virus, Cucumber mosaic virus (CMV). Repression of TOM1 and TOM3 genes by synthetic artificial tasiRNAs in Arabidopsis reproduced the Tobacco mosaic virus-resistant phenotype of tom1/tom3 double mutant, strongly suggesting that we can identify host factors of plant viruses by using the established forward genetic screening system. I then set up the screening to identify host factors, which localize on vacuole membrane or are involved in phospholipid metabolism, required for CMV replication.
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Report
(5 results)
Research Products
(31 results)
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[Journal Article] Roles and programming of Arabidopsis ARGONAUTE proteins during Turnip mosaic virus infection2015
Author(s)
Garcia-Ruiz H., Carbonell A., Hoyer S. J., Fahlgren N., Gilbert K. B., Takeda A., Giampetruzzi A., Garcia-Ruiz M. T., McGinn M. G., Lowery N., Baladejo M. T. M. and Carrington J. C.
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Journal Title
PLoS Pathogens
Volume: 11
Issue: 3
Pages: e1004755-e1004755
DOI
Related Report
Peer Reviewed / Open Access
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