Analysis of Casparian strip formation and apoplastic transport pathway

• Project/Area Number: 26712008
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: The University of Tokyo
• Principal Investigator: Kamiya Takehiro 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (40579439)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
• Keywords: カスパリー線 / スベリン / イオノーム / アポプラスト / 転写因子

Outline of Final Research Achievements

Plants take up essential nutrients and water through root in soil. On the other hand, they prevent non-essential substance from entering into plants. This is enabled by endodermis carrying Casparian strip and suberin. However, their mechanisms of formation and physiological function remain unclear. In out work, we have identified novel protein involved in Casparian strip formation. In addition, through the analysis of that mutant, we have discovered novel function of suberin: Suberin functions as an apoplastic barrier at the site of lateral root where Casparian strip is disrupted.

Research Products

• [Int'l Joint Research] ローザンヌ大学(スイス)
• [Int'l Joint Research] アバディーン大学(英国)
• [Int'l Joint Research] ローザンヌ大学(スイス)
• [Int'l Joint Research] アバディーン大学(英国)
• [Journal Article] Role of LOTR1 in nutrient transport through organization of spatial distribution of root endodermal barriers. (2017)
  • Author(s): *Li, B., *Kamiya, T. , Kalmbach, L., Yamagami, M., Yamaguchi, K., Shuji, S., Sawa, S., Danku, J.M.C, Salt, D.E., Geldner, N., and Fujiwara, T
  • Journal Title: Current Biology Volume: 27 Issue: 5 Pages: 1-8
  • DOI: 10.1016/j.cub.2017.01.030
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 根における栄養吸収と拡散障壁の形成 (2016)
  • Author(s): 神谷岳洋
  • Journal Title: 植物科学の最前線 (BSJ-Review) Volume: 7 Pages: 210-219
  • Peer Reviewed / Open Access
• [Journal Article] The Arabidopsis Mg transporter, MRS2-4, is essential for Mg homeostasis under both low and high Mg conditions. (2016)
  • Author(s): Oda, K., Kamiya, T., Shikanai, Y., Shigenobu, S., Yamaguchi, K., Fujiwara, T.
  • Journal Title: Plant Cell Physiol. Volume: 印刷中 Issue: 4 Pages: 754-763
  • DOI: 10.1093/pcp/pcv196
  • Peer Reviewed / Open Access
• [Journal Article] Large-scale profiling of brown rice ionome in an ethyl methanesulphonate-mutagenized hitomebore population and identification of high- and low-cadmium lines. (2016)
  • Author(s): Nobuhiro Tanaka, Sho Nishida, Takehiro Kamiya and Toru Fujiwara
  • Journal Title: Plant and Soil Volume: in press Issue: 1-2 Pages: 109-117
  • DOI: 10.1007/s11104-016-2812-6
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Differential role of PIN1 and PIN2 in root meristem maintenance under low-B conditions in Arabidopsis thaliana. (2015)
  • Author(s): Li, K., Kamiya, T., Fujiwara, T.
  • Journal Title: Plant Cell Physiol. Volume: 56 Issue: 6 Pages: 1205-1214
  • DOI: 10.1093/pcp/pcv047
  • Peer Reviewed / Open Access
• [Journal Article] An Arabidopsis thaliana copper-sensitive mutant suggests a role of phytosulfokine in ethylene production (2015)
  • Author(s): Tao Wu, Takehiro Kamiya, Hiroko Yumoto, Naoyuki Sotta, Yamaguchi Katsushi, Shuji Shigenobu, Yoshikatsu Matsubayashi and Toru Fujiwara
  • Journal Title: Journal of experimental botany Volume: 66 Issue: 13 Pages: 3657-3667
  • DOI: 10.1093/jxb/erv105
  • Peer Reviewed / Open Access
• [Journal Article] The MYB36 transcription factor orchestrates Casparian strip formation. (2015)
  • Author(s): Kamiya, T., Borghi, M., Wang, P., Danku, J. M., Kalmbach, L., Hosmani, PS., Naseer, S., Fujiwara, T., Geldner, N., Salt, DE.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 112 Issue: 33 Pages: 10533-10538
  • DOI: 10.1073/pnas.1507691112
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Arabidopsis thaliana PRL1 is involved in low calcium tolerance. (2015)
  • Author(s): Shikanai, Y., Yamagami, M., Shigenobu, S., Yamaguchi, K., Kamiya, T., Fujiwara, T.
  • Journal Title: Soil Sci. Plant Nutr. Volume: 61 Issue: 6 Pages: 951-956
  • DOI: 10.1080/00380768.2015.1086277
  • Peer Reviewed / Open Access
• [Journal Article] A receptor-like kinase mutant with absent endodermal diffusion barrier displays selective nutrient homeostasis defects. (2014)
  • Author(s): Pfister, A., Barberon, M., Alassimone, J., Kalmbach, L., Lee, Y., Vermeer, J. E., Yamazaki, M., Li, G., Maurel, C., Takano, J., Kamiya, T., Salt, D. E., Roppolo, D., and Geldner, D.
  • Journal Title: eLife Volume: 3 Pages: 1-20
  • DOI: 10.7554/elife.03115
  • Peer Reviewed / Open Access
• [Presentation] Apoplastic barrier formation and function in roots (2017)
  • Author(s): 神谷岳洋
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] カスパリー線の機能と形成 (2016)
  • Author(s): 神谷岳洋
  • Organizer: 第61回リグニン討論会
  • Place of Presentation: 京都大学宇治キャンパス（京都府宇治市）
  • Year and Date: 2016-10-28
  • Invited
• [Presentation] カスパリー線とスベリン：内皮細胞における拡散障壁 (2016)
  • Author(s): 神谷岳洋
  • Organizer: 第45回根研究集会
  • Place of Presentation: 岡山大学資源植物科学研究所（岡山県倉敷市）
  • Year and Date: 2016-09-30
  • Invited
• [Presentation] 第57回植物生理学会 (2016)
  • Author(s): Baohai Li, Takehiro Kamiya, Lothar Kalmbach, Mutsumi Yamagami, Katsushi Yamaguchi, Shuji Shigenobu, Shinichiro Sawa, John M. C. Danku, David E. Salt, Niko Geldner, Toru Fujiwara
  • Organizer: The analysis of low Ca sensitive mutant reveals coordinated formation of apoplastic barriers by Casparian strip and suberin.
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] カスパリー線形成を制御する転写因子の同定 (2015)
  • Author(s): 神谷岳洋、 John M. Danku、Monica Borghi、Sadaf Naseer、Niko Geldner、藤原徹、David E. Salt
  • Organizer: 日本土壌肥料学会2015年度京都大会
  • Place of Presentation: 京都大学（京都府京都市）
  • Year and Date: 2015-09-09
• [Presentation] カスパリー線の機能と形成機構 (2015)
  • Author(s): 神谷岳洋、 John M. Danku、Monica Borghi、Sadaf Naseer、Niko Geldner、藤原徹、David E. Salt
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] カスパリー線の機能と形成機構 (2015)
  • Author(s): 神谷岳洋
  • Organizer: 第１回植物の栄養研究会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-09-04
  • Invited
• [Presentation] カスパリー線形成のマスターレギュレーターの同定 (2015)
  • Author(s): 神谷岳洋、 John M. Danku、Monica Borghi、Sadaf Naseer、Niko Geldner、藤原徹、David E. Salt
  • Organizer: 第10回トランスポーター研究会年会
  • Place of Presentation: 慶応義塾大学（東京都港区）
  • Year and Date: 2015-06-20
• [Presentation] Identification of the master regulator of Casparian strip formation in Arabidopsis (2015)
  • Author(s): Takehiro Kamiya, John Danku, Monica Borghi, Sadaf Naseer, Niko Geldner, Toru Fujiwara, David Salt
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-16
• [Presentation] Molecular mechanisms of Casparian strip development and its effect on mineral transport. (2014)
  • Author(s): 神谷岳洋
  • Organizer: 土壌肥料学会
  • Place of Presentation: 東京農工大学（東京都小金井市）
  • Year and Date: 2014-09-11
• [Remarks] 土からの栄養吸収に重要な、根の細胞間の隙間をふさぐ仕組みを解明 －植物の栄養輸送の新しい仕組み－
  • URL: http://www.a.u-tokyo.ac.jp/topics/2016/20170227_1.html
• [Remarks] 根のバリアを形成するスイッチ
  • URL: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/switch-for-building-barrier-in-roots.html
• [Remarks] 植物の栄養吸収の要となる構造形成を制御するスイッチを発見
  • URL: http://www.a.u-tokyo.ac.jp/topics/2015/20150701-2.html