Comprehensive analysis of cellular heterogeneity in a clonal microbial population
| Project/Area Number |
26712011
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Miyazaki Ryo 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (80712489)
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| Research Collaborator |
VAN DER MEER Jan R. University of Lausanne, Fundamental Microbiology, Professor
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2016: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2014: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | 遺伝子水平伝播 / 遺伝子発現 / 微生物 / 一細胞解析 / 不均一性 / トランスクリプトーム / ゲノム |
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| Outline of Final Research Achievements |
Recent advanced single-cell studies increasingly reveals large variations in gene expressions or cellular phenotypes at individual cell level even in an isogenic population. However, a challenge is to develop systematic approaches to address the mechanisms to generate such cellular heterogeneities or to detect the physiological differences between individual cells. Using horizontal gene transfer between prokaryotic cells as a model, we here developed a genome-wide approach to analyze gene expression profiles between subpopulations in a clonal population, and uncovered specific cellular physiology and gene expressions in a particular cell fraction.
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Report
(4 results)
Research Products
(7 results)
