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Characterization of flavonoid transport system and its application for the production of useful compoundsb

Research Project

Project/Area Number 26712013
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Applied biochemistry
Research InstitutionKyoto University

Principal Investigator

Sugiyama Akifumi  京都大学, 生存圏研究所, 准教授 (20598601)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywordsフラボノイド / 輸送体 / 有用物質生産 / プラスチド
Outline of Final Research Achievements

Plants produce more than 200,000 secondary metabolites, among which flavonoids are a group of over 7,000 compounds. These compounds are important for our lives as pharmaceutical raw materials and functional foods. However, useful secondary metabolites derived from these plants have a large limitation to stable supply because of low contents in plants, origin from rare plant, difficulty in purification. In this study, we aimed to construct stable flavonoid production system using transformants by clarifying transporters of flavonoids.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (18 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (4 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 4 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] ロレーヌ大学(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] スタンフォード大学(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] スタンフォード大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] ロレーヌ大学(フランス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Molecular Characterization of LjSWEET3, a Sugar Transporter in Nodules of Lotus japonicus2017

    • Author(s)
      Akifumi Sugiyama, Yuka Saida, Mayuko Yoshimizu, Kojiro Takanashi, Davide Sosso, Wolf B Frommer, Kazufumi Yazaki
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Pages: 298-306

    • DOI

      10.1093/pcp/pcw190

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Molecular assessment of the bacterial community associated with Cassava (Manihot esculenta Crantz) cultivation in Cameroon2017

    • Author(s)
      Sarr, P.S., Sugiyama, A., Begoudeb, A.D.B, Yazaki, K.,, Araki, S., Nawata, W.
    • Journal Title

      Microbiological Research

      Volume: 197 Pages: 22-28

    • DOI

      10.1016/j.micres.2016.12.011

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Developmental and nutritional regulation of isoflavone secretion from soybean roots2016

    • Author(s)
      Sugiyama, A., Yamazaki, Y., Yamashita, K., Takahashi, S., Nakayama, T., Yazaki, K.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Issue: 1 Pages: 89-94

    • DOI

      10.1080/09168451.2015.1062714

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular characterization of LjABCG1, an ATP-binding cassette protein in Lotus japonicus.2015

    • Author(s)
      Sugiyama, A., Fukuda, S., Takanashi, K., Yoshioka, M., Yoshioka, H., Narusaka, Y., Narusaka, M., Kojima, M., Sakakibara, H., Shitan, N., Sato, S., Tabata, S., Kawaguchi, M. and Yazaki, K.
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 9 Pages: e0139127-e0139127

    • DOI

      10.1371/journal.pone.0139127

    • NAID

      120005715333

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metabolic engineering of flavonoids with prenyltransferase and chalcone isomerase genes in tomato fruits2014

    • Author(s)
      Kawasaki, K., Koeduka, T., Sugiyama, A., Sasaki, K., Linley, PJ., Shitan, S., Kumano, T., Yamamoto, H., Ezura, H., Kuzuyama, T., Yazaki, K.
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 5 Pages: 567-571

    • DOI

      10.5511/plantbiotechnology.14.0918a

    • NAID

      130004713024

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Flavonoids in plant rhizospheres: secretion, fate and their effects on biological communication2014

    • Author(s)
      Akifumi Sugiyama, Kazufumi Yazaki
    • Journal Title

      Plant Biotechnology

      Volume: 31 Issue: 5 Pages: 431-443

    • DOI

      10.5511/plantbiotechnology.14.0917a

    • NAID

      130004704196

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] プラストキノン・トコフェロール生合成におけるフェノール性中間体の プラスチド取り込み輸送体の探索2017

    • Author(s)
      棟方涼介、杉山暁史、矢崎一史
    • Organizer
      日本農芸化学会 2017年度大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] ダイズ根からのイソフラボン分泌と根圏での動態2016

    • Author(s)
      杉山暁史、山崎由実、濱本昌一郎、高瀬尚文、矢崎一史
    • Organizer
      植物微生物研究会第26回交流会
    • Place of Presentation
      仙台
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] プラストキノン・トコフェロール生合成における フェノール性中間体のプラスチド内向き輸送体の生化学的解析2016

    • Author(s)
      棟方 涼介、杉山 暁史、矢﨑 一史
    • Organizer
      第34回日本植物細胞分子生物学会(上田)大会
    • Place of Presentation
      上田
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular evolution and function of plant-derived prenyltransferases for polyphenols2016

    • Author(s)
      Ryosuke Munakata, Tomoya Takemura, Sakihito Kitajima, Toki Taira, Akifumi Sugiyama, Kazufumi Yazaki
    • Organizer
      11th national meeting of the SFBV
    • Place of Presentation
      Angers (France)
    • Year and Date
      2016-07-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 圃場環境下のダイズにおける生育過程を通じたフラボノイドの含量、分泌及び動態の解析2016

    • Author(s)
      山崎 由実, 杉山 暁史, 高瀬 尚文, 矢崎 一史
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      盛岡
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 圃場栽培ダイズにおける生育過程を通じたフラボノイドの分泌及び動態の解析2015

    • Author(s)
      山崎 由実, 杉山 暁史, 高瀬 尚文, 矢崎 一史
    • Organizer
      第25回植物微生物研究交流会
    • Place of Presentation
      つくば
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] ダイズ圃場での根圏微生物と植物フラボノイドの解析2015

    • Author(s)
      杉山 暁史
    • Organizer
      第8 回ダイズ研究会
    • Place of Presentation
      盛岡市
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ダイズの生育過程における根のフラボノイド及び根圏微生物叢の変動2014

    • Author(s)
      杉山暁史、上田義勝、小野愛、吉川正巳、高瀬尚文、矢崎一史
    • Organizer
      第24回植物微生物研究会
    • Place of Presentation
      佐賀市
    • Year and Date
      2014-09-19
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2022-02-16  

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