| Budget Amount *help |
¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
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| Outline of Final Research Achievements |
Transient gene expression technology, in which a gene encoding high-value protein such as vaccine antigen is introduced into mature plants for transient expression, is attracting interest as a rapid mass production platform for biopharmaceutical proteins. In this study, we aimed at analyzing photosynthetic gas exchange characteristics in leaves and developing a technique for monitoring non-destructively changes in the target protein accumulation level in leaves. We could not find appropriate conditions for chlorophyll fluorescence measurements that enable quantitative monitoring. However, it was shown that, by measuring leaf temperature continuously, days post gene introduction at which time the amount of target protein reaches its maximum level can be estimated, irrespective of air temperature during plant growth.
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