| Budget Amount *help |
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2016: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
|
|---|
| Outline of Final Research Achievements |
An efficient culture system for spermatogonial stem cells of animals except for rodent has not been developed, although it is about fifteen years from first establishment of culture system for mouse spermatogonial stem cells. In this study, we have tried to develop an efficient maintenance and expansion for wide variety of animals using fragmentation of spermatogonial cysts by abscission of intercellular bridges which is evolutionarily well conserved from invertebrate to vertebrate. We have generated an spermatogonial stem cells in which fragmentation of spermatogonial cysts is visualized. In addition, molecular mechanisms which could be responsible for abscission of intercellular bridges were analyzed using germ cell specific JMJD3 deficient mice in which fragmentation of spermatogonial cysts was frequently occurred. A prospective molecular mechanisms which could regulate fragmentation of spermatogonial cysts has be identified.
|
