Establishment of an efficient maintenance and expansion system for spermatogonial stem cells from wide variety of animals.
| Project/Area Number |
26712026
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kyushu University |
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Principal Investigator |
IWAMORI Naoki 九州大学, 大学院農学研究院, 准教授 (70647362)
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| Research Collaborator |
IWAMORI Tokuko 九州大学, 大学院医学研究院, 助教 (10711509)
SHIMA Sakurako
YAMAMURA Yuki
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2016: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Keywords | 精子幹細胞 / 細胞間架橋 / エピジェネティクス / JMJD3 / 生殖細胞 / エピジェネティックス / 生殖生物学 / 幹細胞 / 細胞培養 |
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| Outline of Final Research Achievements |
An efficient culture system for spermatogonial stem cells of animals except for rodent has not been developed, although it is about fifteen years from first establishment of culture system for mouse spermatogonial stem cells. In this study, we have tried to develop an efficient maintenance and expansion for wide variety of animals using fragmentation of spermatogonial cysts by abscission of intercellular bridges which is evolutionarily well conserved from invertebrate to vertebrate. We have generated an spermatogonial stem cells in which fragmentation of spermatogonial cysts is visualized. In addition, molecular mechanisms which could be responsible for abscission of intercellular bridges were analyzed using germ cell specific JMJD3 deficient mice in which fragmentation of spermatogonial cysts was frequently occurred. A prospective molecular mechanisms which could regulate fragmentation of spermatogonial cysts has be identified.
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] Astrocytic and neuronal localization of kunurenine aminotransferase-2 in the adult mouse brain.2016
Author(s)
Heredi J, Berko AM, Jankovics F, Iwamori T, Iwamori N, Ono E, Horvath S, Kis Z, Toldi J, Vecsei L, Gellert L.
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Journal Title
Brain Struct Funct
Volume: 印刷中
Issue: 4
Pages: 1663-1672
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Systemic L-Kynurenine sulfate administration disrupts object recognition memory, alters open field behavior and decreases c-Fos immunopositivity in C57Bl/6 mice.2015
Author(s)
Varga D, Heredi J, Kanvasi Z, Ruszka M, Kis Z, Ono E, Iwamori N, Iwamori T, Takakuwa H, Vecsei L, Toldi J, Gellert L.
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Journal Title
Front Behav Neurosci
Volume: 9
Pages: 157-157
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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