Genetic basis underlying evolution of sex pheromone preference in the silkmoth and its related species

• Project/Area Number: 26712027
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Insect science
• Research Institution: Tokyo University of Agriculture (2018); The University of Tokyo (2014-2017)
• Principal Investigator: SAKURAI Takeshi 東京農業大学, 農学部, 准教授 (20506761)
• Research Collaborator: DAIMON Takaaki ; MITSUNO Hidefumi
• Project Period (FY): 2014-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 昆虫 / 性フェロモン / 生殖隔離 / カイコガ科 / フェロモン選好性

Outline of Final Research Achievements

In the present study, we first verified that a sex pheromone receptor is the　determinant factor for pheromone preference in male silkmoths by in vivo functional analyses of sex pheromone receptor and pheromone binding protein gene knockout silkmoths. Then, we revealed response specificity of pheromone sensitive ORNs and functionally characterized ligands of sex pheromone receptors in related bombycidae species. From comparison between selectivity of ORNs and ligand specificity of sex pheromone receptors, we obtained an important insight into relationships between function of sex pheromone receptors and evolution of pheromone preference in male bombycidae moths.

Academic Significance and Societal Importance of the Research Achievements

ガ類の性フェロモン交信系は化学物質を介した種分化の研究モデルとして注目されている。本研究では、フェロモン受容のモデル昆虫であるカイコガとその近縁種のフェロモン受容体、フェロモン受容細胞の応答性を明らかにし種間比較を行うことで、フェロモン選好性とフェロモン受容体の進化に関する知見を拡張した点に意義がある。また本研究は、ゲノム編集によりフェロモン受容に関与する遺伝子ノックアウト体を作出することで、その生体内での機能を初めて明らかにした点においても学術的な意義がある。

Research Products

• [Journal Article] In vivo functional characterisation of pheromone binding protein-1 in the silkmoth, Bombyx mori (2018)
  • Author(s): Shiota Yusuke、Sakurai Takeshi、Daimon Takaaki、Mitsuno Hidefumi、Fujii Takeshi、Matsuyama Shigeru、Sezutsu Hideki、Ishikawa Yukio、Kanzaki Ryohei
  • Journal Title: Scientific Reports Volume: 8 Issue: 1
  • DOI: 10.1038/s41598-018-31978-2
  • NAID: 120007133757
  • Peer Reviewed / Open Access
• [Journal Article] Lipid droplets in the pheromone gland of wild silkmoth <i>Bombyx mandarina</i> (2018)
  • Author(s): Fujii Takeshi, Sakurai Takeshi, Ito Katsuhiko, Yokoyama Takeshi, Kanzaki Ryohei
  • Journal Title: Journal of Insect Biotechnology and Sericology Volume: 87 Issue: 2 Pages: 2_029-2_034
  • DOI: 10.11416/jibs.87.2_029
  • NAID: 130007431359
  • ISSN: 1346-8073, 1884-7978
  • Peer Reviewed / Open Access
• [Journal Article] ガ類の高選択的・高感度な性フェロモン受容の分子機構 (2018)
  • Author(s): 櫻井健志
  • Journal Title: 昆虫と自然 Volume: 53 Pages: 13-17
• [Journal Article] Lipid droplets in the pheromone gland of wild silkmoth Bombyx mandarina (2018)
  • Author(s): Takeshi Fujii, Takeshi Sakurai, Ito K, Yokoyama T, Kanzaki R
  • Journal Title: Journal of Insect Biotechnology & Sericology Volume: -
  • NAID: 130007431359
  • Peer Reviewed
• [Journal Article] 匂いセンサカイコガ開発の可能性 (2017)
  • Author(s): 櫻井健志、光野秀文、神崎亮平
  • Journal Title: アグリバイオ Volume: 1 Pages: 641-646
• [Journal Article] Establishment of a specific cell death induction system in Bombyx mori by a transgene with the conserved apoptotic regulator, mouse Bcl-2-associated X protein (mouse Bax). (2015)
  • Author(s): M. Sumitani, T. Sakurai, Katsumi Kasashima, S. Kobayashi, K. Uchino, R. Kanzaki, T. Tamura, and H. Sezutsu
  • Journal Title: Insect Molecular Biology Volume: 24 Issue: 6 Pages: 671-680
  • DOI: 10.1111/imb.12192
  • Peer Reviewed
• [Journal Article] Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth (2015)
  • Author(s): Sakurai T, Mitsuno H, Mikami A, Uchino K, Tabuchi M, Zhang F, Sezutsu H, Kanzaki R
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 11001-11001
  • DOI: 10.1038/srep11001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] カイコガの高選択・高感度な性フェロモン認識の分子・神経基盤 (2014)
  • Author(s): 櫻井健志、神崎亮平
  • Journal Title: Sanshi-Konchu Biotec Volume: 83 Issue: 2 Pages: 2_115-2_127
  • DOI: 10.11416/konchubiotec.83.2_115
  • NAID: 130004705722
  • ISSN: 1881-0551, 1884-7943
  • Peer Reviewed
• [Presentation] フェロモン結合タンパク質遺伝子ノックアウトカイコガの触角葉内でのフェロモン受容神経の応答キネティクス解析 (2019)
  • Author(s): 塩田裕介、櫻井健志、内野恵郎、瀬筒秀樹、神崎亮平
  • Organizer: 第63回日本応用動物昆虫学会大会
• [Presentation] Analysis of temporal antennal response kinetics associated with efficient pheromone source localization in the silkmoth, Bombyx mori (2018)
  • Author(s): Shiota Yusuke、Sakurai Takeshi、Ando Noriyasu, Haupt Stephan Shuichi, Mitsuno Hidefumi, Daimon Takaaki、Kanzaki Ryohei
  • Organizer: 日本比較生理生化学会第40回大会
• [Presentation] 雄カイコガの触角応答キネティクスと効率的なフェロモン源探索行動の解析 (2018)
  • Author(s): 1塩田裕介, 櫻井健志, 安藤規泰, 光野秀文, 大門高明、神崎亮平
  • Organizer: 第62回日本応用動物昆虫学会大会
  • Invited
• [Presentation] 遺伝子組換えカイコガを利用したフェロモン受容体遺伝子の発現制御領域の探索 (2017)
  • Author(s): 櫻井健志、田渕理史、内野恵郎、瀬筒秀樹、神崎亮平
  • Organizer: 第61回日本応用動物昆虫学会
  • Place of Presentation: 東京農工大、東小金井
  • Year and Date: 2017-03-27
• [Presentation] フェロモン結合タンパク質遺伝子ノックアウトカイコガの神経生理応答のキネティクス・フェロモン源探索行動解析 (2017)
  • Author(s): 塩田裕介、櫻井健志、安藤規泰、光野秀文、神崎亮平
  • Organizer: 第61回日本応用動物昆虫学会
  • Place of Presentation: 東京農工大、東小金井
  • Year and Date: 2017-03-27
• [Presentation] カイコガのフェロモン受容システムを利用した匂いセンサ昆虫の開発 (2017)
  • Author(s): 櫻井健志、神崎亮平
  • Organizer: 平成29年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 農林水産技術会議事務局、つくば
  • Year and Date: 2017-03-21
  • Invited
• [Presentation] Molecular and neural mechanisms underlying efficient pheromone source localization in the silkmoth Bombyx mor (2017)
  • Author(s): 櫻井健志、塩田裕介、神崎亮平
  • Organizer: 日本比較生理生化学会第39回福岡大会
  • Invited
• [Presentation] In vivo functional analysis of genes involved in sex pheromone detection in the silkmoth Bombyx mori (2017)
  • Author(s): Takeshi Sakurai, Yusuke Shiota, Takaaki Daimon, Miwa Uchibori-Asano, Keiro Uchino, Hidefumi Mitsuno, Hideki Sezutsu, Ryohei Kanzaki
  • Organizer: The joint meeting of the 33rd annual meeting of the ISCE and the 9th meeting of the APACE
  • Int'l Joint Research / Invited
• [Presentation] Analysis of relationship between antennal response kinetics and pheromone source localization efficiency in pheromone binding protein knockout silkmoth, Bombyx mori (2017)
  • Author(s): Yusuke Shiota, Takeshi Sakurai, Noriyasu Ando, Hidefumi Mitsuno, Takaaki Daimon, Ryohei Kanzaki
  • Organizer: 日本比較生理科学会第39回福岡大会
• [Presentation] 昆虫の嗅覚メカニズムを利用した匂いセンサ (2016)
  • Author(s): 櫻井健志
  • Organizer: eビジネス異業種交流会
  • Place of Presentation: 一般財団法人 マルチメディア振興センター会議室、東京
  • Year and Date: 2016-10-06
  • Invited
• [Presentation] 昆虫嗅覚受容体の機能的再構成系の構築と匂いセンサへの応用 (2016)
  • Author(s): 櫻井健志、光野秀文、神崎亮平
  • Organizer: 第68回日本生物工学会年次大会
  • Place of Presentation: 富山国際会議場、富山
  • Year and Date: 2016-09-28
  • Invited
• [Presentation] Temporal kinetics analysis of pheromone responses of pheromone binding protein knockout silkmoth, Bombyx mori (2016)
  • Author(s): Yusuke Shiota, Takeshi Sakurai, Noriyasu Ando, Takaaki Daimon, Ryohei Kanzaki
  • Organizer: 第38回日本比較生理生化学会
  • Place of Presentation: 玉川大学、町田
  • Year and Date: 2016-09-02
• [Presentation] Development of insect antenna-based odorant sensor (2016)
  • Author(s): Takeshi Sakurai, Ryohei Kanzaki
  • Organizer: Japanese Society of Comparative Physiology and Biochemistry mini-symposium “Environmental Sensing and Behavior”
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2016-06-10
  • Int'l Joint Research / Invited
• [Presentation] Physiological and behavioral analysis of pheromone binding protein gene knockout silkmoth (2016)
  • Author(s): Takeshi Sakurai, Yusuke Shiota, Takaaki Daimon, Hidefumi Mitsuno, Ryohei Kanzaki
  • Organizer: The 17th International Symposium of Olfaction and Taste
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2016-06-05
  • Int'l Joint Research
• [Presentation] フェロモン結合タンパク質遺伝子ノックアウトカイコガの生理・行動解析 (2016)
  • Author(s): 櫻井健志、塩田裕介、大門高明、光野秀文、神崎亮平
  • Organizer: 第60回日本応用動物昆虫学会
  • Place of Presentation: 大阪府立大学、堺
  • Year and Date: 2016-03-26
• [Presentation] Molecular and neural mechanisms of sex pheromone perception in the silkmoth Bombyx mori (2015)
  • Author(s): 櫻井健志
  • Organizer: CompBiol2015 広島大会
  • Place of Presentation: JMSアステールプラザ、広島
  • Year and Date: 2015-12-11
  • Invited
• [Presentation] Establishment and behavioral analysis of pheromone binding protein gene knockout silkmoth (2015)
  • Author(s): Takeshi Sakurai, Yusuke Shiota, Takaaki Daimon, Hidefumi Mitsuno, Ryohei Kanzaki
  • Organizer: CompBiol2015 広島大会
  • Place of Presentation: JMSアステールプラザ、広島
  • Year and Date: 2015-12-11
• [Presentation] A single sex pheromone receptor mediates bombykol responses in the silkmoth (2015)
  • Author(s): Takeshi Sakurai, Kanzaki Ryohei
  • Organizer: 8th Asia-Pacific Chemical Ecology Conference
  • Place of Presentation: California, USA
  • Year and Date: 2015-09-23
  • Int'l Joint Research / Invited
• [Presentation] Genetic manipulation of odorant response of the silkmoth antennae (2015)
  • Author(s): Takeshi Sakurai, Kanzaki Ryohei
  • Organizer: Symposium in HFSP kickoff meeting
  • Place of Presentation: Konstanz, Germany
  • Year and Date: 2015-07-27
  • Int'l Joint Research / Invited
• [Presentation] 遺伝子組換えカイコガを利用した性フェロモン受容体遺伝子のin vivoプロモーター解析 (2014)
  • Author(s): 櫻井健志、田渕理史、水野依利子、瀬筒秀樹、神崎亮平
  • Organizer: 日本味と匂い学会第48回大会
  • Place of Presentation: 清水文化会館マリナーレ、静岡
  • Year and Date: 2014-10-02 – 2014-10-04
• [Book] 昆虫の脳をつくる (2018)
  • Author(s): 並木重宏、櫻井健志（神崎亮平編著）
  • Total Pages: 224
  • Publisher: 朝倉書店
  • ISBN: 9784254102772
• [Book] Essentials of Machine Olfaction and Taste (2016)
  • Author(s): Ryohei Kanzaki, Kei Nakatani, Takeshi Sakurai, Nobuo Misawa, Hidefumi Mitsuno
  • Total Pages: 336
  • Publisher: Wiley