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Genetic basis underlying evolution of sex pheromone preference in the silkmoth and its related species

Research Project

Project/Area Number 26712027
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Insect science
Research InstitutionTokyo University of Agriculture (2018)
The University of Tokyo (2014-2017)

Principal Investigator

SAKURAI Takeshi  東京農業大学, 農学部, 准教授 (20506761)

Research Collaborator DAIMON Takaaki  
MITSUNO Hidefumi  
Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords昆虫 / 性フェロモン / 生殖隔離 / カイコガ科 / フェロモン選好性
Outline of Final Research Achievements

In the present study, we first verified that a sex pheromone receptor is the determinant factor for pheromone preference in male silkmoths by in vivo functional analyses of sex pheromone receptor and pheromone binding protein gene knockout silkmoths. Then, we revealed response specificity of pheromone sensitive ORNs and functionally characterized ligands of sex pheromone receptors in related bombycidae species. From comparison between selectivity of ORNs and ligand specificity of sex pheromone receptors, we obtained an important insight into relationships between function of sex pheromone receptors and evolution of pheromone preference in male bombycidae moths.

Academic Significance and Societal Importance of the Research Achievements

ガ類の性フェロモン交信系は化学物質を介した種分化の研究モデルとして注目されている。本研究では、フェロモン受容のモデル昆虫であるカイコガとその近縁種のフェロモン受容体、フェロモン受容細胞の応答性を明らかにし種間比較を行うことで、フェロモン選好性とフェロモン受容体の進化に関する知見を拡張した点に意義がある。また本研究は、ゲノム編集によりフェロモン受容に関与する遺伝子ノックアウト体を作出することで、その生体内での機能を初めて明らかにした点においても学術的な意義がある。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (30 results)

All 2019 2018 2017 2016 2015 2014

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (20 results) (of which Int'l Joint Research: 5 results,  Invited: 10 results) Book (2 results)

  • [Journal Article] In vivo functional characterisation of pheromone binding protein-1 in the silkmoth, Bombyx mori2018

    • Author(s)
      Shiota Yusuke、Sakurai Takeshi、Daimon Takaaki、Mitsuno Hidefumi、Fujii Takeshi、Matsuyama Shigeru、Sezutsu Hideki、Ishikawa Yukio、Kanzaki Ryohei
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1

    • DOI

      10.1038/s41598-018-31978-2

    • NAID

      120007133757

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lipid droplets in the pheromone gland of wild silkmoth <i>Bombyx mandarina</i>2018

    • Author(s)
      Fujii Takeshi, Sakurai Takeshi, Ito Katsuhiko, Yokoyama Takeshi, Kanzaki Ryohei
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 87 Issue: 2 Pages: 2_029-2_034

    • DOI

      10.11416/jibs.87.2_029

    • NAID

      130007431359

    • ISSN
      1346-8073, 1884-7978
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ガ類の高選択的・高感度な性フェロモン受容の分子機構2018

    • Author(s)
      櫻井健志
    • Journal Title

      昆虫と自然

      Volume: 53 Pages: 13-17

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Lipid droplets in the pheromone gland of wild silkmoth Bombyx mandarina2018

    • Author(s)
      Takeshi Fujii, Takeshi Sakurai, Ito K, Yokoyama T, Kanzaki R
    • Journal Title

      Journal of Insect Biotechnology & Sericology

      Volume: -

    • NAID

      130007431359

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 匂いセンサカイコガ開発の可能性2017

    • Author(s)
      櫻井健志、光野秀文、神崎亮平
    • Journal Title

      アグリバイオ

      Volume: 1 Pages: 641-646

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Establishment of a specific cell death induction system in Bombyx mori by a transgene with the conserved apoptotic regulator, mouse Bcl-2-associated X protein (mouse Bax).2015

    • Author(s)
      M. Sumitani, T. Sakurai, Katsumi Kasashima, S. Kobayashi, K. Uchino, R. Kanzaki, T. Tamura, and H. Sezutsu
    • Journal Title

      Insect Molecular Biology

      Volume: 24 Issue: 6 Pages: 671-680

    • DOI

      10.1111/imb.12192

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth2015

    • Author(s)
      Sakurai T, Mitsuno H, Mikami A, Uchino K, Tabuchi M, Zhang F, Sezutsu H, Kanzaki R
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 11001-11001

    • DOI

      10.1038/srep11001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] カイコガの高選択・高感度な性フェロモン認識の分子・神経基盤2014

    • Author(s)
      櫻井健志、神崎亮平
    • Journal Title

      Sanshi-Konchu Biotec

      Volume: 83 Issue: 2 Pages: 2_115-2_127

    • DOI

      10.11416/konchubiotec.83.2_115

    • NAID

      130004705722

    • ISSN
      1881-0551, 1884-7943
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] フェロモン結合タンパク質遺伝子ノックアウトカイコガの触角葉内でのフェロモン受容神経の応答キネティクス解析2019

    • Author(s)
      塩田裕介、櫻井健志、内野恵郎、瀬筒秀樹、神崎亮平
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of temporal antennal response kinetics associated with efficient pheromone source localization in the silkmoth, Bombyx mori2018

    • Author(s)
      Shiota Yusuke、Sakurai Takeshi、Ando Noriyasu, Haupt Stephan Shuichi, Mitsuno Hidefumi, Daimon Takaaki、Kanzaki Ryohei
    • Organizer
      日本比較生理生化学会第40回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 雄カイコガの触角応答キネティクスと効率的なフェロモン源探索行動の解析2018

    • Author(s)
      1塩田裕介, 櫻井健志, 安藤規泰, 光野秀文, 大門高明、神崎亮平
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 遺伝子組換えカイコガを利用したフェロモン受容体遺伝子の発現制御領域の探索2017

    • Author(s)
      櫻井健志、田渕理史、内野恵郎、瀬筒秀樹、神崎亮平
    • Organizer
      第61回日本応用動物昆虫学会
    • Place of Presentation
      東京農工大、東小金井
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] フェロモン結合タンパク質遺伝子ノックアウトカイコガの神経生理応答のキネティクス・フェロモン源探索行動解析2017

    • Author(s)
      塩田裕介、櫻井健志、安藤規泰、光野秀文、神崎亮平
    • Organizer
      第61回日本応用動物昆虫学会
    • Place of Presentation
      東京農工大、東小金井
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] カイコガのフェロモン受容システムを利用した匂いセンサ昆虫の開発2017

    • Author(s)
      櫻井健志、神崎亮平
    • Organizer
      平成29年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      農林水産技術会議事務局、つくば
    • Year and Date
      2017-03-21
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Molecular and neural mechanisms underlying efficient pheromone source localization in the silkmoth Bombyx mor2017

    • Author(s)
      櫻井健志、塩田裕介、神崎亮平
    • Organizer
      日本比較生理生化学会第39回福岡大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] In vivo functional analysis of genes involved in sex pheromone detection in the silkmoth Bombyx mori2017

    • Author(s)
      Takeshi Sakurai, Yusuke Shiota, Takaaki Daimon, Miwa Uchibori-Asano, Keiro Uchino, Hidefumi Mitsuno, Hideki Sezutsu, Ryohei Kanzaki
    • Organizer
      The joint meeting of the 33rd annual meeting of the ISCE and the 9th meeting of the APACE
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of relationship between antennal response kinetics and pheromone source localization efficiency in pheromone binding protein knockout silkmoth, Bombyx mori2017

    • Author(s)
      Yusuke Shiota, Takeshi Sakurai, Noriyasu Ando, Hidefumi Mitsuno, Takaaki Daimon, Ryohei Kanzaki
    • Organizer
      日本比較生理科学会第39回福岡大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 昆虫の嗅覚メカニズムを利用した匂いセンサ2016

    • Author(s)
      櫻井健志
    • Organizer
      eビジネス異業種交流会
    • Place of Presentation
      一般財団法人 マルチメディア振興センター会議室、東京
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 昆虫嗅覚受容体の機能的再構成系の構築と匂いセンサへの応用2016

    • Author(s)
      櫻井健志、光野秀文、神崎亮平
    • Organizer
      第68回日本生物工学会年次大会
    • Place of Presentation
      富山国際会議場、富山
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Temporal kinetics analysis of pheromone responses of pheromone binding protein knockout silkmoth, Bombyx mori2016

    • Author(s)
      Yusuke Shiota, Takeshi Sakurai, Noriyasu Ando, Takaaki Daimon, Ryohei Kanzaki
    • Organizer
      第38回日本比較生理生化学会
    • Place of Presentation
      玉川大学、町田
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of insect antenna-based odorant sensor2016

    • Author(s)
      Takeshi Sakurai, Ryohei Kanzaki
    • Organizer
      Japanese Society of Comparative Physiology and Biochemistry mini-symposium “Environmental Sensing and Behavior”
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-06-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Physiological and behavioral analysis of pheromone binding protein gene knockout silkmoth2016

    • Author(s)
      Takeshi Sakurai, Yusuke Shiota, Takaaki Daimon, Hidefumi Mitsuno, Ryohei Kanzaki
    • Organizer
      The 17th International Symposium of Olfaction and Taste
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2016-06-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] フェロモン結合タンパク質遺伝子ノックアウトカイコガの生理・行動解析2016

    • Author(s)
      櫻井健志、塩田裕介、大門高明、光野秀文、神崎亮平
    • Organizer
      第60回日本応用動物昆虫学会
    • Place of Presentation
      大阪府立大学、堺
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Molecular and neural mechanisms of sex pheromone perception in the silkmoth Bombyx mori2015

    • Author(s)
      櫻井健志
    • Organizer
      CompBiol2015 広島大会
    • Place of Presentation
      JMSアステールプラザ、広島
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Establishment and behavioral analysis of pheromone binding protein gene knockout silkmoth2015

    • Author(s)
      Takeshi Sakurai, Yusuke Shiota, Takaaki Daimon, Hidefumi Mitsuno, Ryohei Kanzaki
    • Organizer
      CompBiol2015 広島大会
    • Place of Presentation
      JMSアステールプラザ、広島
    • Year and Date
      2015-12-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] A single sex pheromone receptor mediates bombykol responses in the silkmoth2015

    • Author(s)
      Takeshi Sakurai, Kanzaki Ryohei
    • Organizer
      8th Asia-Pacific Chemical Ecology Conference
    • Place of Presentation
      California, USA
    • Year and Date
      2015-09-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetic manipulation of odorant response of the silkmoth antennae2015

    • Author(s)
      Takeshi Sakurai, Kanzaki Ryohei
    • Organizer
      Symposium in HFSP kickoff meeting
    • Place of Presentation
      Konstanz, Germany
    • Year and Date
      2015-07-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 遺伝子組換えカイコガを利用した性フェロモン受容体遺伝子のin vivoプロモーター解析2014

    • Author(s)
      櫻井健志、田渕理史、水野依利子、瀬筒秀樹、神崎亮平
    • Organizer
      日本味と匂い学会第48回大会
    • Place of Presentation
      清水文化会館マリナーレ、静岡
    • Year and Date
      2014-10-02 – 2014-10-04
    • Related Report
      2014 Annual Research Report
  • [Book] 昆虫の脳をつくる2018

    • Author(s)
      並木重宏、櫻井健志(神崎亮平編著)
    • Total Pages
      224
    • Publisher
      朝倉書店
    • ISBN
      9784254102772
    • Related Report
      2018 Annual Research Report
  • [Book] Essentials of Machine Olfaction and Taste2016

    • Author(s)
      Ryohei Kanzaki, Kei Nakatani, Takeshi Sakurai, Nobuo Misawa, Hidefumi Mitsuno
    • Total Pages
      336
    • Publisher
      Wiley
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2020-03-30  

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