| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2014: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
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| Outline of Final Research Achievements |
We found that the bile-mediated activation of the acrAB and tolC multidrug efflux genes occurs mainly through transcriptional derepression of ramA in Salmonella. It was revealed that multidrug efflux pumps contribute to E. coli biofilm maintenance. It was discovered that AcrB, AcrD, and MdtABC multidrug efflux systems are involved in enterobactin export. We also investigated the effect of methylglyoxal on multidrug-resistant P. aeruginosa, and roles of Salmonella multidrug efflux pumps in decreased susceptibility to triclosan. The structures of the regulator bound with bile acids were also solved, and we performed the phenotype microarray analysis of the drug efflux systems in Salmonella enterica serovar Typhimurium.
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