| Budget Amount *help |
¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
This study aims to generate a testicular organoid by creating a seminiferous tubule which is a site of spermatogenesis in de novo. Therefore, We tried to induce differentiation from pluripotent stem cells to Sertoli cells, the main constituent cells of seminiferous tubules. First, Sox9-EGFP ES cells were established from mice in which EGFP was knocked-in to Sertoli cell-specific gene Sox9. Furthermore, a vector incorporating three transcription factors into a tetracycline induction system was constructed and introduced into Sox9-GFP ES cells. We succeeded in inducing a large number of Sox9-EGFP expressing cells by overexpressing transcription factors at an appropriate timing after inducing differentiation with growth factors.
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