Generation of testicular organoids from pluripotent stem cells
Project/Area Number |
26713012
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Partial Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Yokohama City University |
Principal Investigator |
Sato Takuya 横浜市立大学, 生命医科学研究科, 特任助教 (70599505)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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Keywords | 体外精子形成誘導 / 精巣オルガノイド / ゲノム編集 / 精子幹細胞 / 器官培養 / セルトリ細胞 / 精子形成 / 精巣 / オルガノイド / 再生医学 / 発生・分化 |
Outline of Final Research Achievements |
This study aims to generate a testicular organoid by creating a seminiferous tubule which is a site of spermatogenesis in de novo. Therefore, We tried to induce differentiation from pluripotent stem cells to Sertoli cells, the main constituent cells of seminiferous tubules. First, Sox9-EGFP ES cells were established from mice in which EGFP was knocked-in to Sertoli cell-specific gene Sox9. Furthermore, a vector incorporating three transcription factors into a tetracycline induction system was constructed and introduced into Sox9-GFP ES cells. We succeeded in inducing a large number of Sox9-EGFP expressing cells by overexpressing transcription factors at an appropriate timing after inducing differentiation with growth factors.
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] In vitro mouse spermatogenesis with an organ culture method in chemically defined medium.2018
Author(s)
Sanjo H, Komeya M, Sato T, Abe T, Katagiri K, Yamanaka H, Ino Y, Arakawa N, Hirano H, Yao T, Asayama Y, Matsuhisa A, Yao M, Ogawa T.
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Journal Title
PLoS One
Volume: 12
Issue: 2
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Pumpless microfluidic system driven by hydrostatic pressure induces and maintains mouse spermatogenesis in vitro.2017
Author(s)
Komeya M, Hayashi K, Nakamura H, Yamanaka H, Sanjo H, Kojima K, Sato T, Yao M, Kimura H, Fujii T, Ogawa T.
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Journal Title
Sci Rep.
Volume: 13
Issue: 1
Pages: 15459-15459
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] SHISA6 Confers Resistance to Differentiation-Promoting Wnt/β-Catenin Signaling in Mouse Spermatogenic Stem Cells.2017
Author(s)
Tokue M, Ikami K, Mizuno S, Takagi C, Miyagi A, Takada R, Noda C, Kitadate Y, Hara K, Mizuguchi H, Sato T, Taketo MM, Sugiyama F, Ogawa T, Kobayashi S, Ueno N, Takahashi S, Takada S, Yoshida S.
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Journal Title
Stem Cell Reports.
Volume: 8
Issue: 3
Pages: 561-575
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device2016
Author(s)
M. Komeya, H. Kimura, H. Nakamura, T. Yokonishi, T. Sato, K. Kojima, K. Hayashi, K. Katagiri, H. Yamanaka, H. Sanjo, M. Yao, S. Kamimura, K. Inoue, N. Ogonuki, A. Ogura, T. Fujii, T. Ogawa
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 21472-21472
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Offspring production with sperm grown in vitro from cryopreserved testis tissues2014
Author(s)
Yokonishi T, Sato T, Komeya M, Katagiri K, Kubota Y, Nakabayashi K, Hata K, Inoue K, Ogonuki N, Ogura A, Ogawa T
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Journal Title
Nature Communications
Volume: 5
Issue: 1
Pages: 4320-4320
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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