Adult common diseases analysis using non-linear in vivo imaging technique

• Project/Area Number: 26713013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Jichi Medical University
• Principal Investigator: Nishimura Satoshi 自治医科大学, 医学部, 教授 (80456136)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000); Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000); Fiscal Year 2014: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
• Keywords: 病態医化学 / 循環器学 / 生体イメージング / 二光子顕微鏡 / 炎症 / 血栓 / 造血

Outline of Final Research Achievements

We developed multi-scale imaging systems to cover micro- and macro findings of living mice, using two photon microscope, and macro-imager, which enabled us to obtain broad-field and high-resolution images. Utilizing these imaging systems, and light-manipulation technique, we revealed molecular mechanisms under adult common diseases. We had advantage for real-time observation of living animals, and additionally proposed new “functional bio-imaging” technique.

Research Products

• [Journal Article] Alendronate inhalation ameliorates elastase-induced pulmonary emphysema in mice by induction of apoptosis of alveolar macrophages (2015)
  • Author(s): Manabu Ueno, et al.
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1-13
  • DOI: 10.1038/ncomms7332
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] IL-1 alpha induces rapid enhanced platelet biogenesis through rupture of Running title: Rupture thrombopoiesis regulated by IL-1alpha murine bone marrow megakaryocytes (2015)
  • Author(s): Satoshi Nishimura, Mika Nagasaki, Shinji Kunishima, Asuka Sakata, Hiroyasu Sakaguchi, Naoya Takayama, Issei Komuro, Joe Italiano Jr., Tomiko Ryu, Tsukasa Ohmori, Takashi Kadowaki, Koji Eto, Ryozo Nagai
  • Journal Title: Journal of cell biology Volume: 未 Issue: 3 Pages: 453-66
  • DOI: 10.1083/jcb.201410052
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Roles of ADAM8 in elimination of injured muscle fibers prior to skeletal muscle regeneration (2015)
  • Author(s): D. Nishimura, H. Sakai, T. Sato, F. Sato, S. Nishimura, N. Toyama-Sorimachi, J. W. Bartsch and A. Sehara-Fujisawa
  • Journal Title: Mech Dev Volume: 135 Pages: 58-67
  • DOI: 10.1016/j.mod.2014.12.001
  • Peer Reviewed / Open Access
• [Journal Article] Tentative title : IL-1alphainduces rapid enhanced platelet biogenesis through rupture of bone marrow megakaryocytes (2015)
  • Author(s): Nishimura S, Nagasaki M, Kunishima S, Sawaguchi A, Sakata A, Sakaguchi H, Ohmori T, Manabe I, J Italiano, Ryu T, Takayama N, Komuro I, Kadowaki T, Eto K, Nagai R.
  • Journal Title: J Cell Biology Volume: in pub
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Alendronate inhalation ameliorates elastase-induced pulmonary emphysema in mice by induction of apoptosis of alveolar macrophages (2015)
  • Author(s): Ueno M, Maeno T, Nishimura S, Ogata F, Masubuchi H, Hara K, Yamaguchi K, Aoki F, Suga T, Nagai R, Kurabayashi M
  • Journal Title: Nat Commumnications, Volume: n pub
  • Peer Reviewed / Open Access
• [Journal Article] Macrophage-inducible C-type lectin underlies obesity-induced adipose tissue fibrosis (2015)
  • Author(s): Tanaka M, Ikeda K, Suganami T, Komiya C, Ochi K, Shirakawa I, Hamaguchi M, Nishimura S, Manabe I, Matsuda T, Kimura K, Inoue H, Inagaki Y, Aoe S, Yamasaki S, Ogawa
  • Journal Title: Nat Commumnications, Volume: n pub
  • Peer Reviewed / Open Access
• [Journal Article] ENPP2 contributes to adipose Tissue expansion and insulin resistance in diet-induced obesity (2014)
  • Author(s): Nishimura S, Nagasaki M, Okudaira S, Aoki J, Ohmori T, Ohkawa R, Nakamura K, Igarashi K, Yamashita H, Eto K, Uno K, Hayashi N, Kadowaki T, Komuro I, Yatomi Y, Nagai R
  • Journal Title: Diabetes, Volume: 63 Pages: 4154-64
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Paxillin is an intrinsic negative regulator of platelet activation in mice. (2014)
  • Author(s): Sakata A, Ohmori T, Nishimura S, Suzuki H, Madoiwa S, Mimuro J, Kario K, Sakata Y.
  • Journal Title: Thromb J. Volume: 12
  • Peer Reviewed / Open Access
• [Journal Article] Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells (2014)
  • Author(s): Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita K, Koike T, Harimoto K, Dohda T, Watanabe A, Okita K, Takahasi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K
  • Journal Title: Cell Stem Cell, Volume: 14 Pages: 535-535
  • Peer Reviewed / Open Access
• [Presentation] Multi-scale in vivo XYZT imaging of single cells by 1P, 2P, and on-chip visualization technique, and post-processing (2016)
  • Author(s): Satoshi Nishimura
  • Organizer: EMBL Workshop From 3D Light to 3D Electron Microscopy
  • Place of Presentation: ドイツ
  • Year and Date: 2016-03-13
  • Int'l Joint Research
• [Presentation] Multi-modal visualization of in vivo XYZT cell dynamics (2016)
  • Author(s): Satoshi Nishimura
  • Organizer: EMBL Workshop VIZBI2016 Heidelberg
  • Place of Presentation: ドイツ
  • Year and Date: 2016-03-13
• [Presentation] 二光子ミクロと自由行動マクロイメージングを用いた生活恒常性と病態解析 (2016)
  • Author(s): 西村智
  • Organizer: レーザー学会学術講演会 第36回年次大会
  • Place of Presentation: 愛知
  • Year and Date: 2016-01-09
  • Invited
• [Presentation] フローサイトメトリーとバイオイメージングの融合～光を用いた分子メカニズムの解明～ (2015)
  • Author(s): 第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
  • Organizer: 西村智
  • Place of Presentation: 兵庫
  • Year and Date: 2015-12-01
• [Presentation] 蛍光生体イメージングでみる心血管恒常性と破綻 (2015)
  • Author(s): 西村智
  • Organizer: 脳心血管抗加齢研究会2015
  • Place of Presentation: 大阪
  • Year and Date: 2015-11-28
  • Invited
• [Presentation] 生体蛍光イメージングでみる心血管・血栓イベント (2015)
  • Author(s): 西村智
  • Organizer: 第29回日本冠疾患学会学術集会
  • Place of Presentation: 北海道
  • Year and Date: 2015-11-20
  • Invited
• [Presentation] 蛍光を用いた生体における血管・血栓・心機能解析(Fluorescent Imaging Analysis of Vessel, Thrombus, and Heart in Living Mice) (2015)
  • Author(s): 西村智
  • Organizer: 第19回日本心不全学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2015-10-22
• [Presentation] The contribution of endothelial damage and platelet age to thrombotic and regenerative processes (2015)
  • Author(s): 西村智、坂田飛鳥、瀬尾欣也、江藤浩之
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: 石川
  • Year and Date: 2015-10-16
• [Presentation] 生体二光子顕微鏡による味覚反応と肥満による変容の可視化解析 (2015)
  • Author(s): 西村智、瀬尾欣也、坂田飛鳥
  • Organizer: 第36回日本肥満学会
  • Place of Presentation: 京都
  • Year and Date: 2015-10-02
• [Presentation] 生体蛍光イメージングでみる心血管・血栓イベント (2015)
  • Author(s): 西村智
  • Organizer: 第29回日本心臓血管内視鏡学会
  • Place of Presentation: 大阪
  • Year and Date: 2015-09-26
  • Invited
• [Presentation] 生体イメージングによる知覚・感覚・恒常性維持の可視化解析 (2015)
  • Author(s): 西村智
  • Organizer: 第47回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2015-09-18
  • Invited
• [Presentation] Intravital Multi-Dimensional Two Photon Imaging Visualizes Inflammatory and Regenerative Responses to Acute Injuries (2015)
  • Author(s): Satoshi Nishimura
  • Organizer: Cell and Developmental Systems
  • Place of Presentation: スイス
  • Year and Date: 2015-08-18
  • Int'l Joint Research
• [Presentation] How to Translate XWZT Microscopic Date to Biological and Medical Researchers? (2015)
  • Author(s): Satoshi Nishimura
  • Organizer: 第18回画像の認識・理解シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2015-07-27
  • Int'l Joint Research
• [Presentation] 蛍光生体イメージングによる血管機能異常の定量的解析 (2015)
  • Author(s): 西村智
  • Organizer: 第47回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 宮城
  • Year and Date: 2015-07-09
• [Presentation] RAPID ‘RUPTURE’-TYPE THROMBOPOIESIS PROCESSES FROM BONE MARROW MEGAKARYOCYTE IN RESPONSE TO ACUTE PLATELET NEEDS IS REGULATED BY IL-1ALPHA (2015)
  • Author(s): Satoshi Nishimura, Asuka Sakata, Kinya Seo, Tsukasa Ohmori, Koji Eto
  • Organizer: The ISTH’s Scientific and Standardization Committee
  • Place of Presentation: カナダ
  • Year and Date: 2015-06-20
  • Int'l Joint Research
• [Presentation] 生体血管イメージングによる疾患理解 血栓・炎症・造血・再生 (2015)
  • Author(s): 第18回北野動脈硬化症セミナー
  • Organizer: 西村智
  • Place of Presentation: 大阪
  • Year and Date: 2015-06-18
• [Presentation] バイオイメージングを用いた血栓止血学研究「みる」と「わかる」 (2015)
  • Author(s): 西村智
  • Organizer: 第37回日本血栓止血学会学術集会
  • Place of Presentation: 山梨
  • Year and Date: 2015-05-21
  • Invited
• [Presentation] 二光子血管イメージングによる定量的・多面的な生体評価系の確立と生活習慣病への応用 (2015)
  • Author(s): 西村 智
  • Organizer: 日本顕微鏡学会 第71回学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2015-05-13
• [Presentation] Fluorescence as an Interactive Tool for Life: From Molecular Mechanisms to Human Cardiovascular Events (2015)
  • Author(s): 西村智
  • Organizer: 第79回日本循環器学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2015-04-24
  • Invited
• [Presentation] Cell-Cell interactions in Diseased Conditions Analyzed by Two Photon, Three Dimensional, and High-Speed in Vivo Microscope: From Visualization to Quantification (2015)
  • Author(s): S.Nishimura
  • Organizer: Novel Techniques in Microscopy2015
  • Place of Presentation: カナダ
  • Year and Date: 2015-04-12
  • Int'l Joint Research
• [Presentation] 非線形顕微鏡を用いた多面的・定量的な生体評価系の確立と応用 (2015)
  • Author(s): 西村 智
  • Organizer: 第52回日本臨床分子医学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2015-04-10
• [Remarks]
  • URL: http://www.invivoimaging.net/