Development of MALDI-MS-based analytical technique for biomarker discovery and diagnosis

• Project/Area Number: 26713020
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Kyushu University
• Principal Investigator: Miura Daisuke 九州大学, 農学研究院, 特任准教授 (40532627)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000); Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 質量分析 / イメージング / メタボロミクス / MALDI / 質量分析イメージング / バイオマーカー / MALDI-MS

Outline of Final Research Achievements

A platform for biomarker discovery and diagnosis with multiple mass spectrometry (MS)-driven metabolite analytical platform were developed. This platform includes, 1. high-throughput metabolite analysis system, 2. spatiotemporally-resolved metabolite analysis system, and 3. standard-independent identification method of elemental composition for metabolite annotation. By accelerating interdisciplinary technical fusion in diverse biomedical redox-related research fields, substantial development of drug discovery tool, diagnostic methodology and instrument is in progress, and thus which lead to the establishment of new discipline of pathological metabolomics.

Research Products

• [Journal Article] Analysis of spatiotemporal metabolomic dynamics for sensitively monitoring biological alterations in cisplatin-induced acute kidney injury. (2018)
  • Author(s): Irie, M., Hayakawa, E., Fujimura, Y., Honda, Y., Setoyama, D., Wariishi, H., Hyodo, F. and Miura, D.*
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 496 Issue: 1 Pages: 140-146
  • DOI: 10.1016/j.bbrc.2018.01.012
  • Peer Reviewed
• [Journal Article] Impact of the Oral Adsorbent AST-120 on organ-specific accumulation of uremic toxins: LC-MS/MS and MS imaging techniques (2018)
  • Author(s): Emiko Sato, Daisuke Saigusa, Eikan Mishima, Taeko Uchida, Daisuke Miura, Tomomi Morikawa-Ichinose, Kiyomi Kisu, Akiyo Sekimoto, Ritsumi Saito, Yuji Oe, Yotaro Matsumoto, Yoshihisa Tomioka, Takefumi Mori, Nobuyuki Takahashi, Hirosi Sato, Takaaki Abe, Toshimitsu Niwa, and Sadayosi Ito
  • Journal Title: Toxins Volume: 10 Issue: 1 Pages: 19-33
  • DOI: 10.3390/toxins10010019
  • Peer Reviewed / Open Access
• [Journal Article] A Chemometrics-driven Strategy for the Bioactivity Evaluation of Complex Multicomponent Systems and the Effective Selection of Bioactivity-predictive Chemical Combinations. (2017)
  • Author(s): Fujimura, Y., Kawano, C., Maeda-Murayama, A., Nakamura, A., Koike-Miki, A., Yukihira, D., Hayakawa, E., Ishii, T., Tachibana, H., Wariishi, H. and Miura, D.*
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 2257-2257
  • DOI: 10.1038/s41598-017-02499-1
  • Peer Reviewed / Open Access
• [Journal Article] Role of Ad4-binding protein/steroidogenic factor 1 in regulating NADPH production in adrenocortical Y-1 cells (2017)
  • Author(s): Li B, BabaT, Miyabayashi K, Sato T, Shima Y, Ichinose T, Miura D, Ohkawa Y, Suyama M, Morohashi KI
  • Journal Title: Endocrine Journal Volume: 64 Issue: 3 Pages: 315-324
  • DOI: 10.1507/endocrj.EJ16-0467
  • NAID: 130005529460
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access
• [Journal Article] Extra-pancreatic invasion induces lipolytic and fibrotic changes in the adipose microenvironment, with released fatty acids enhancing the invasiveness of pancreatic cancer cells. (2017)
  • Author(s): Okumura T, Ohuchida K, Sada M, Abe T, Endo S, Koikawa K, Iwamoto C, Miura D, Mizuuchi Y, Moriyama T, Nakata K, Miyasaka Y, Manabe T, Ohtsuka T, Nagai E, Mizumoto K, Oda Y, Hashizume M, Nakamura M.
  • Journal Title: Oncotarget Volume: 8 Issue: 11 Pages: 18280-18295
  • DOI: 10.18632/oncotarget.15430
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Spatially resolved metabolic distribution for unraveling the physiological change and responses in tomato fruit using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) (2017)
  • Author(s): Nakamura J, Morikawa-Ichinose T, Fujimura Y, Hayakawa E, Takahashi K, Ishii T, Miura D, and Wariishi H
  • Journal Title: Anal Bioanal Chem Volume: 409 Issue: 6 Pages: 1697-1706
  • DOI: 10.1007/s00216-016-0118-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The 1,2-diaminocyclohexane carrier ligand in oxaliplatin induces p53-dependent transcriptional repression of factors involved in thymidylate biosynthesis. (2015)
  • Author(s): Kiyonari S, Iimori M, Matsuoka K, Watanabe S, Morikawa-Ichinose T, Miura D, Niimi S, Saeki H, Tokunaga E, Oki E, Morita M, Kadomatsu K, Maehara Y, Kitao H
  • Journal Title: Mol Cancer Ther Volume: 14 Issue: 10 Pages: 2332-42
  • DOI: 10.1158/1535-7163.mct-14-0748
  • Peer Reviewed / Open Access
• [Journal Article] Power of Isotopic Fine Structure for Unambiguous Determination of Metabolite Elemental Compositions: in silico Evaluation and Metabolomic Application (2014)
  • Author(s): Nagao T., Yukihira D., Fujimura Y., Saito K., Takahashi K., Miura D. and Wariishi H.
  • Journal Title: Analytica Chimica Acta Volume: 813 Pages: 70-76
  • DOI: 10.1016/j.aca.2014.01.032
  • Peer Reviewed / Open Access
• [Journal Article] MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals (2014)
  • Author(s): Fujimura Y and Miura D
  • Journal Title: Metabolites Volume: 4 Issue: 2 Pages: 319-346
  • DOI: 10.3390/metabo4020319
  • Peer Reviewed / Open Access
• [Presentation] Establishment of convenient sample preparation method with high sensitivity and excellent reproducibility in mass spectrometry imaging of endogenous primary metabolites (2017)
  • Author(s): Tomomi Ichinose; Fusa Murayama; Takanori Ishii; Chihiro Kawano; Ayaka Murayama; Akiko Miki; Yoshinori Fujimura; Yuzo Yamazaki; Hiroyuki Wariishi; DAISUKE MIURA
  • Organizer: 65th Conference on Mass Spectrometry and Allied Topics, American Society for Mass Spectrometry
  • Int'l Joint Research
• [Presentation] Structure-Property Relationship of MALDI Matrix for Low-Molecular-Weight Metabolites Analysis (2016)
  • Author(s): Daisuke Miura, Takanori Ishii, Daichi Yukihira, Yoshinori Fujimura, Hiroyuki Wariishi, Mitsuru Shindo
  • Organizer: 21st International Mass Spectrometry Conference
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2016-08-20
  • Int'l Joint Research
• [Presentation] Structure-Property Relationship Strategic Synthesis of MALDI Matrix for Low-Molecular-Weight Metabolites Analysis (2016)
  • Author(s): Daisuke Miura, Takanori Ishii, Daichi Yukihira, Yoshinori Fujimura, Hiroyuki Wariishi, Mitsuru Shindo
  • Organizer: 65th American Society for Mass Spectrometry
  • Place of Presentation: San Antonio, USA
  • Year and Date: 2016-06-05
  • Int'l Joint Research
• [Presentation] MS/MS スペクトルネットワークと化合物データベースによる代謝物構造決定法の開発 (2015)
  • Author(s): 早川英介・藤村由紀・三浦大典
  • Organizer: 第67回日本生物工学会大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2015-10-26
• [Presentation] 時空間分解代謝解析技術の基礎・臨床医学への応用 (2015)
  • Author(s): 三浦大典
  • Organizer: 第33回内分泌代謝学サマーセミナー
  • Place of Presentation: 福岡
  • Year and Date: 2015-07-11
• [Presentation] MALDI-MS-Based Metabolomic Imaging for Visualizing Spatiotemporal Metabolic Behaviors. (2015)
  • Author(s): Daisuke Miura
  • Organizer: 17th The Scientific International Symposium on SIMS and Related Techniques Based on Ion-Solid Interactions
  • Place of Presentation: 東京
  • Year and Date: 2015-06-25
  • Int'l Joint Research / Invited
• [Presentation] MALDIマトリックスの構造機能相関と戦略的合成展開 (2015)
  • Author(s): 三浦大典・ 石井孝典・ 早川英介・ 藤村由紀・ 割石博之・ 新藤充
  • Organizer: 第63回質量分析総合討論会
  • Place of Presentation: つくば
  • Year and Date: 2015-06-17
• [Presentation] Functional Metabolic Multimodality Imaging by Dynamic Nuclear Polarization-Magnetic Resonance Imaging and Mass Spectrometry Imaging. (2015)
  • Author(s): Miura, D., Hyodo, F., and Fujimura, Y.
  • Organizer: 63rd ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: St.Louis, MO
  • Year and Date: 2015-05-31
  • Int'l Joint Research
• [Presentation] 時空間分解メタボロミクスの基礎・臨床医学への応用 (2015)
  • Author(s): 三浦大典
  • Organizer: 第88回日本内分泌学会
  • Place of Presentation: 東京
  • Year and Date: 2015-04-23
  • Invited
• [Presentation] MALDI-MS-based metabolomic imaging technique and its application to pathological analysis. (2014)
  • Author(s): Daisuke Miura
  • Organizer: RERF symposium “Application of ‘omics’ to Radiation Research”
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2014-12-02 – 2014-12-03
  • Invited
• [Presentation] MALDI-MS-based metabolite analysis for biomarker discovery and diagnosis. (2014)
  • Author(s): Daisuke Miura
  • Organizer: CIS workshop “Mass spectrometry for proteomics and imaging”
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2014-10-23 – 2014-10-24
  • Invited
• [Presentation] Development of MALDI-MS-based metabolomic imaging technique and its application to pathological analysis. (2014)
  • Author(s): Daisuke Miura
  • Organizer: DFG-JSPS Symposium “SHARED PATHWAYS IN CNS SIDORDERS”
  • Place of Presentation: Munich, Germany
  • Year and Date: 2014-06-29 – 2014-06-30
  • Invited