• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of MALDI-MS-based analytical technique for biomarker discovery and diagnosis

Research Project

Project/Area Number 26713020
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Laboratory medicine
Research InstitutionKyushu University

Principal Investigator

Miura Daisuke  九州大学, 農学研究院, 特任准教授 (40532627)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords質量分析 / イメージング / メタボロミクス / MALDI / 質量分析イメージング / バイオマーカー / MALDI-MS
Outline of Final Research Achievements

A platform for biomarker discovery and diagnosis with multiple mass spectrometry (MS)-driven metabolite analytical platform were developed. This platform includes, 1. high-throughput metabolite analysis system, 2. spatiotemporally-resolved metabolite analysis system, and 3. standard-independent identification method of elemental composition for metabolite annotation. By accelerating interdisciplinary technical fusion in diverse biomedical redox-related research fields, substantial development of drug discovery tool, diagnostic methodology and instrument is in progress, and thus which lead to the establishment of new discipline of pathological metabolomics.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015 2014

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 8 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results)

  • [Journal Article] Analysis of spatiotemporal metabolomic dynamics for sensitively monitoring biological alterations in cisplatin-induced acute kidney injury.2018

    • Author(s)
      Irie, M., Hayakawa, E., Fujimura, Y., Honda, Y., Setoyama, D., Wariishi, H., Hyodo, F. and Miura, D.*
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 496 Issue: 1 Pages: 140-146

    • DOI

      10.1016/j.bbrc.2018.01.012

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of the oral adsorbent AST-120 on organ-specific accumulation of uremic toxins: LC-MS/MS and MS imaging techniques2018

    • Author(s)
      Emiko Sato, Daisuke Saigusa, Eikan Mishima, Taeko Uchida, Daisuke Miura, Tomomi Morikawa-Ichinose, Kiyomi Kisu, Akiyo Sekimoto, Ritsumi Saito, Yuji Oe, Yotaro Matsumoto, Yoshihisa Tomioka, Takefumi Mori, Nobuyuki Takahashi, Hirosi Sato, Takaaki Abe, Toshimitsu Niwa, and Sadayosi Ito
    • Journal Title

      Toxins

      Volume: 10 Issue: 1 Pages: 19-33

    • DOI

      10.3390/toxins10010019

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Chemometrics-driven Strategy for the Bioactivity Evaluation of Complex Multicomponent Systems and the Effective Selection of Bioactivity-predictive Chemical Combinations.2017

    • Author(s)
      Fujimura, Y., Kawano, C., Maeda-Murayama, A., Nakamura, A., Koike-Miki, A., Yukihira, D., Hayakawa, E., Ishii, T., Tachibana, H., Wariishi, H. and Miura, D.*
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 2257-2257

    • DOI

      10.1038/s41598-017-02499-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of Ad4-binding protein/steroidogenic factor 1 in regulating NADPH production in adrenocortical Y-1 cells2017

    • Author(s)
      Li B, BabaT, Miyabayashi K, Sato T, Shima Y, Ichinose T, Miura D, Ohkawa Y, Suyama M, Morohashi KI
    • Journal Title

      Endocrine Journal

      Volume: 64 Issue: 3 Pages: 315-324

    • DOI

      10.1507/endocrj.EJ16-0467

    • NAID

      130005529460

    • ISSN
      0918-8959, 1348-4540
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Extra-pancreatic invasion induces lipolytic and fibrotic changes in the adipose microenvironment, with released fatty acids enhancing the invasiveness of pancreatic cancer cells.2017

    • Author(s)
      Okumura T, Ohuchida K, Sada M, Abe T, Endo S, Koikawa K, Iwamoto C, Miura D, Mizuuchi Y, Moriyama T, Nakata K, Miyasaka Y, Manabe T, Ohtsuka T, Nagai E, Mizumoto K, Oda Y, Hashizume M, Nakamura M.
    • Journal Title

      Oncotarget

      Volume: 8 Issue: 11 Pages: 18280-18295

    • DOI

      10.18632/oncotarget.15430

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Spatially resolved metabolic distribution for unraveling the physiological change and responses in tomato fruit using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI)2017

    • Author(s)
      Nakamura J, Morikawa-Ichinose T, Fujimura Y, Hayakawa E, Takahashi K, Ishii T, Miura D, and Wariishi H
    • Journal Title

      Anal Bioanal Chem

      Volume: 409 Issue: 6 Pages: 1697-1706

    • DOI

      10.1007/s00216-016-0118-4

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The 1,2-diaminocyclohexane carrier ligand in oxaliplatin induces p53-dependent transcriptional repression of factors involved in thymidylate biosynthesis.2015

    • Author(s)
      Kiyonari S, Iimori M, Matsuoka K, Watanabe S, Morikawa-Ichinose T, Miura D, Niimi S, Saeki H, Tokunaga E, Oki E, Morita M, Kadomatsu K, Maehara Y, Kitao H
    • Journal Title

      Mol Cancer Ther

      Volume: 14 Issue: 10 Pages: 2332-42

    • DOI

      10.1158/1535-7163.mct-14-0748

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Power of Isotopic Fine Structure for Unambiguous Determination of Metabolite Elemental Compositions: in silico Evaluation and Metabolomic Application2014

    • Author(s)
      Nagao T., Yukihira D., Fujimura Y., Saito K., Takahashi K., Miura D. and Wariishi H.
    • Journal Title

      Analytica Chimica Acta

      Volume: 813 Pages: 70-76

    • DOI

      10.1016/j.aca.2014.01.032

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] MALDI Mass Spectrometry Imaging for Visualizing In Situ Metabolism of Endogenous Metabolites and Dietary Phytochemicals2014

    • Author(s)
      Fujimura Y and Miura D
    • Journal Title

      Metabolites

      Volume: 4 Issue: 2 Pages: 319-346

    • DOI

      10.3390/metabo4020319

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Establishment of convenient sample preparation method with high sensitivity and excellent reproducibility in mass spectrometry imaging of endogenous primary metabolites2017

    • Author(s)
      Tomomi Ichinose; Fusa Murayama; Takanori Ishii; Chihiro Kawano; Ayaka Murayama; Akiko Miki; Yoshinori Fujimura; Yuzo Yamazaki; Hiroyuki Wariishi; DAISUKE MIURA
    • Organizer
      65th Conference on Mass Spectrometry and Allied Topics, American Society for Mass Spectrometry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structure-Property Relationship of MALDI Matrix for Low-Molecular-Weight Metabolites Analysis2016

    • Author(s)
      Daisuke Miura, Takanori Ishii, Daichi Yukihira, Yoshinori Fujimura, Hiroyuki Wariishi, Mitsuru Shindo
    • Organizer
      21st International Mass Spectrometry Conference
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2016-08-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Structure-Property Relationship Strategic Synthesis of MALDI Matrix for Low-Molecular-Weight Metabolites Analysis2016

    • Author(s)
      Daisuke Miura, Takanori Ishii, Daichi Yukihira, Yoshinori Fujimura, Hiroyuki Wariishi, Mitsuru Shindo
    • Organizer
      65th American Society for Mass Spectrometry
    • Place of Presentation
      San Antonio, USA
    • Year and Date
      2016-06-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] MS/MS スペクトルネットワークと化合物データベースによる代謝物構造決定法の開発2015

    • Author(s)
      早川英介・藤村由紀・三浦大典
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 時空間分解代謝解析技術の基礎・臨床医学への応用2015

    • Author(s)
      三浦大典
    • Organizer
      第33回内分泌代謝学サマーセミナー
    • Place of Presentation
      福岡
    • Year and Date
      2015-07-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] MALDI-MS-Based Metabolomic Imaging for Visualizing Spatiotemporal Metabolic Behaviors.2015

    • Author(s)
      Daisuke Miura
    • Organizer
      17th The Scientific International Symposium on SIMS and Related Techniques Based on Ion-Solid Interactions
    • Place of Presentation
      東京
    • Year and Date
      2015-06-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] MALDIマトリックスの構造機能相関と戦略的合成展開2015

    • Author(s)
      三浦大典・ 石井孝典・ 早川英介・ 藤村由紀・ 割石博之・ 新藤充
    • Organizer
      第63回質量分析総合討論会
    • Place of Presentation
      つくば
    • Year and Date
      2015-06-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional Metabolic Multimodality Imaging by Dynamic Nuclear Polarization-Magnetic Resonance Imaging and Mass Spectrometry Imaging.2015

    • Author(s)
      Miura, D., Hyodo, F., and Fujimura, Y.
    • Organizer
      63rd ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      St.Louis, MO
    • Year and Date
      2015-05-31
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 時空間分解メタボロミクスの基礎・臨床医学への応用2015

    • Author(s)
      三浦大典
    • Organizer
      第88回日本内分泌学会
    • Place of Presentation
      東京
    • Year and Date
      2015-04-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] MALDI-MS-based metabolomic imaging technique and its application to pathological analysis.2014

    • Author(s)
      Daisuke Miura
    • Organizer
      RERF symposium “Application of ‘omics’ to Radiation Research”
    • Place of Presentation
      Hiroshima, Japan
    • Year and Date
      2014-12-02 – 2014-12-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] MALDI-MS-based metabolite analysis for biomarker discovery and diagnosis.2014

    • Author(s)
      Daisuke Miura
    • Organizer
      CIS workshop “Mass spectrometry for proteomics and imaging”
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2014-10-23 – 2014-10-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of MALDI-MS-based metabolomic imaging technique and its application to pathological analysis.2014

    • Author(s)
      Daisuke Miura
    • Organizer
      DFG-JSPS Symposium “SHARED PATHWAYS IN CNS SIDORDERS”
    • Place of Presentation
      Munich, Germany
    • Year and Date
      2014-06-29 – 2014-06-30
    • Related Report
      2014 Annual Research Report
    • Invited

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi