| Project/Area Number |
26713038
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Chiba University |
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Principal Investigator |
MATSUOKA YUMI 千葉大学, 医学(系)研究科(研究院), 助教 (10402067)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2015: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2014: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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| Keywords | アトピー性皮膚炎 / 黄色ブドウ球菌 / クオラムセンシング / 細菌 |
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| Outline of Final Research Achievements |
We found that Myd88-/- mice showed no skin inflammation, whereas WT mice showed severe eczematous lesions with the similar epicutaneous colonization of S. aureus. K14-CreMyd88-/- mice, the skin inflammation was also dramatically reduced, indicating the involvement of MyD88 in KC. We next applied S. aureus on Il1r-/- mice. When applied on Il1r-/- mice with IL-36R Ab, the inflammation was significantly reduced, showing additive IL-36 involvement. Upon the infection, little IL-17 were detected in K14-CreMyd88-/-, Il1r-/- with IL-36RAb mice. In accordance with these, Il17-/- mice showed significantly less skin inflammation upon the infection.We also found that an exotoxin beside secreted from S. aureus was essential in our epicutaneous mouse model. Similar with d-toxin, the expression of this exotoxin is also controlled by agr-quorum sensing in S. aureus indicates that controlling the quorum sensing in S. aureus may be an important therapeutic target of atopic dermatitis.
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