| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Outline of Final Research Achievements |
This study aimed to fabricate 3-Dimensional functional cardiac tissue from human iPS derived cardiomyocytes which treats human failed heart. We focused on and utilize well known phenomenon spheroid formation of cell. Human iPS cell derived cardiomyocytes, human vascular endothelial cells, fibroblasts, and neural cell were fused to form contractile cardiac spheroids. Firstly, we optimize the cell amount, combination ratio, and another cell source to create better contractile spheroid. We found the addition of iPS cell derived neural cell increase function of cardiac spheroid. Second, we developed 3-Dimensional fabrication system. Utilizing this system, spheroids were easily arranged to fabricate cardiovascular patch construct, and tubular consturact. Combing spheroids phenomenon and tissue engineering devices, these constructs containe enough extracelluar matrix, microvascular networks in the construct.
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