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Fabrication of 3-dimensional functional cardiac tissue from human iPS cell derived cardiomyocyets for heart regeneration

Research Project

Project/Area Number 26713044
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Cardiovascular surgery
Research InstitutionKumamoto University (2016-2017)
Saga University (2014-2015)

Principal Investigator

Noguchi Ryo  熊本大学, 医学部附属病院, 助教 (70530187)

Research Collaborator MUKAE Yosuke  佐賀大学, 医学部心臓血管外科
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Keywords再生医療 / 心筋再生 / 心不全治療 / 組織工学 / 幹細胞 / 心不全外科 / 心臓外科 / 再生医学 / ヒト幹細胞 / iPS細胞
Outline of Final Research Achievements

This study aimed to fabricate 3-Dimensional functional cardiac tissue from human iPS derived cardiomyocytes which treats human failed heart. We focused on and utilize well known phenomenon spheroid formation of cell. Human iPS cell derived cardiomyocytes, human vascular endothelial cells, fibroblasts, and neural cell were fused to form contractile cardiac spheroids. Firstly, we optimize the cell amount, combination ratio, and another cell source to create better contractile spheroid. We found the addition of iPS cell derived neural cell increase function of cardiac spheroid. Second, we developed 3-Dimensional fabrication system. Utilizing this system, spheroids were easily arranged to fabricate cardiovascular patch construct, and tubular consturact. Combing spheroids phenomenon and tissue engineering devices, these constructs containe enough extracelluar matrix, microvascular networks in the construct.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (6 results)

All 2018 2017 2016 2015

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] The addition of human iPS cell-derived neural progenitors changes the contraction of human iPS cell-derived cardiac spheroids.2018

    • Author(s)
      Yosuke Mukae、Ryo Noguchi et al
    • Journal Title

      Tissue and Cell

      Volume: Available online 5 May 2018 Pages: 61-67

    • DOI

      10.1016/j.tice.2018.05.002

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of a three-dimensional pre-vascularized scaffold-free contractile cardiac patch for treating heart disease.2016

    • Author(s)
      Noguchi R, Nakayama K, Itoh M, Kamohara K, Furukawa K, Oyama J, Node K, Morita S.
    • Journal Title

      J Heart Lung Transplant

      Volume: 35 Issue: 1 Pages: 137-45

    • DOI

      10.1016/j.healun.2015.06.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 内胸動脈の細胞を立体化して新たに内胸動脈を作る基盤技術開発2018

    • Author(s)
      野口亮、古賀亜由美、隈元清仁、田爪宏和、岡本健、福井寿啓
    • Organizer
      B2Bカンファレンス2018
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞のみから機能的3次元心臓血管組織を構築する次世代型心臓血管外科再生医療技術開発2017

    • Author(s)
      野口亮
    • Organizer
      B2Bconference
    • Place of Presentation
      東京
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 組織体形成装置及び組織体形成キット2018

    • Inventor(s)
      野口亮
    • Industrial Property Rights Holder
      野口亮
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 特願2014-1047262015

    • Inventor(s)
      野口亮
    • Industrial Property Rights Holder
      佐賀大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-104726
    • Filing Date
      2015-05-20
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2019-03-29  

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