Crosstalk between immune system and nervous system in the pathogenesis of spinal cord injury
| Project/Area Number |
26713047
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
KOHYAMA JUN 慶應義塾大学, 医学部(信濃町), 准教授 (30437511)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | 脊髄損傷 / Notchシグナル / エピジェネティクス / 神経科学 / オリゴデンドロサイト前駆細胞 |
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| Outline of Final Research Achievements |
To unravel mechanistic insight in progression of spinal cord injury, I focused on the interaction between the immune system and the nervous system. I initially discovered activation of Sox2 exprssion in the injured site and,importantly, the Sox2-positive cells were surrounded by microglial cells. To further clarify the mechanism of Sox2 upregulation, we characterized the cells and found that these cells were oligodendrocyte precursor cells (OPCs). At the site of spinal cord injury, immune cell accumulation was observed after injury, and these immune cells expressed ligand for Notch signaling. Then, proliferation of OPCs was activated by Notch signaling. Furthermore, conditional knockout of RBP-J, an effector of Notch signaling, in OPCs resulted in defect in activation of OPCs, indicating that interaction between immune system and nervous system, which was mediated by Notch signaling is important for pathogenesis of spinal cord injury.
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Report
(5 results)
Research Products
(6 results)
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[Journal Article] Whole-Genome DNA Methylation Analyses Revealed Epigenetic Instability in Tumorigenic Human iPS Cell-Derived Neural Stem/Progenitor Cells.2017
Author(s)
Iida T, Iwanami A, Sanosaka T, Kohyama J, Miyoshi H, Nagoshi N, Kashiwagi R, Toyama Y, Matsumoto M, Nakamura M, Okano H.
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Journal Title
Stem Cells
Volume: 35
Issue: 5
Pages: 1316-1327
DOI
Related Report
Peer Reviewed
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[Journal Article] Grafted human iPS cell-derived oligodendrocyte precursor cells contribute to robust remyelination of demyelinated axons after spinal cord injury2016
Author(s)
Kawabata S, Takano M, Numasawa-Kuroiwa Y, Itakura G, Kobayashi Y, Nishiyama Y, Sugai K, Nishimura S, Iwai H, Isoda M, Shibata S, Kohyama J, Iwanami A, Toyama Y, Matsumoto M, Nakamura M, Okano H,
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Journal Title
Stem Cell Reports
Volume: 6(1)
Issue: 1
Pages: 1-8
DOI
Related Report
Peer Reviewed / Open Access
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