| Budget Amount *help |
¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2015: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Outline of Final Research Achievements |
The ultimate goal of this project is to understand the mechanism underlying the Hedgehog (Hh)-Gli-mediated specification of progenitors into bone-forming osteoblasts, in terms of dynamics of epigenome, transcription, and gene regulatory networks. Cell populations being at the specification phase were identified in the osteoblast differentiation system of mouse embryonic stem cells, and molecular characteristics of the populations were determined. Findings on molecular mechanisms underlying the Hh-Gli-mediated osteoblast specification were obtained by chromatin immunoprecipitation sequencing (ChIP-seq) and transcriptional profiling in the identified population. These findings will help us establish the genome-wide molecular basis of efficacy and safety of Hh signaling in bone regenerative therapies.
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