Dynamics of epigenome and transcription during the Hedgehog signaling-mediated specification of skeletal cells
| Project/Area Number |
26713054
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Ohba Shinsuke 東京大学, 大学院医学系研究科(医学部), 准教授 (20466733)
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| Research Collaborator |
HOJO Hironori
MCMAHON Andrew
HE Xinjun
TEI Yuichi
KANKE Kosuke
KITAURA Yoshiaki
KASHIWAGI Miki
ZUJUR Denise
YAMAKAWA Akira
PERMATASARI Galuh Wening
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2015: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Keywords | ヘッジホッグ / 骨形成 / シグナル伝達 / 発生分化 / 生体分子 |
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| Outline of Final Research Achievements |
The ultimate goal of this project is to understand the mechanism underlying the Hedgehog (Hh)-Gli-mediated specification of progenitors into bone-forming osteoblasts, in terms of dynamics of epigenome, transcription, and gene regulatory networks. Cell populations being at the specification phase were identified in the osteoblast differentiation system of mouse embryonic stem cells, and molecular characteristics of the populations were determined. Findings on molecular mechanisms underlying the Hh-Gli-mediated osteoblast specification were obtained by chromatin immunoprecipitation sequencing (ChIP-seq) and transcriptional profiling in the identified population. These findings will help us establish the genome-wide molecular basis of efficacy and safety of Hh signaling in bone regenerative therapies.
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Report
(5 results)
Research Products
(44 results)
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[Presentation] Stepwise differentiation of pluripotent stem cells into osteoblasts using four small molecules under serum-free and feeder-free conditions2014
Author(s)
Kanke K, Masaki H, Saito S, Komiyama Y, Hojo H, Lichtler AC, Nakauchi H, Takato T, Chung UI, Ohba S
Organizer
International Society for Stem Cell Research 12th Annual Meeting
Place of Presentation
Vancouver, Canada
Year and Date
2014-06-20
Related Report
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