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Rescue of arrested replication forks by multiple ubiquitination of PCNA

Research Project

Project/Area Number 26740017
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation and chemicals
Research InstitutionNagoya University

Principal Investigator

Kanao Rie  名古屋大学, 環境医学研究所, 助教 (30542287)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsDNA損傷トレランス / PCNA / モノユビキチン化 / 損傷乗り越え複製 / 翻訳後修飾
Outline of Final Research Achievements

DNA damage tolerance is an important mechanism to prevent DNA replication arrest by DNA damage. It is suggested that DNA damage tolerance is regulated by post-translational modifications of PCNA at K164. PCNA forms a ring-shaped structure consist of homo-trimeric PCNA molecules, which means a single PCNA ring have three modifiable K164. In this study, I investigated the mechanism of DNA damage tolerance pathway which is regulated by simultaneously mono-ubiquitinated PCNA on three K164 in a ring. I analyzed the responses of the cells expressing ectopic PCNA mutated at K164 to DNA damaging agents.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 5 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Journal Article] Different types of interaction between PCNA and PIP boxes contribute to distinct cellular functions of Y-family DNA polymerases.2015

    • Author(s)
      Masuda Y, Kanao R, Kaji K, Ohmori H, Hanaoka F, Masutani C
    • Journal Title

      Nucleic Acids Res

      Volume: 43 Issue: 16 Pages: 7898-7910

    • DOI

      10.1093/nar/gkv712

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] USP7 is a suppressor of PCNA ubiquitination and oxidative-stress-induced mutagenesis in human cells.2015

    • Author(s)
      Kashiwaba S, Kanao R, Masuda Y, Kusumoto-Matsuo R, Hanaoka F, Masutani C
    • Journal Title

      Cell Rep

      Volume: 13 Issue: 10 Pages: 2072-2080

    • DOI

      10.1016/j.celrep.2015.11.014

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Relevance of simultaneous mono-ubiquitinations of multiple units of PCNA homo-trimers in DNA damage tolerance.2015

    • Author(s)
      Kanao R, Masuda Y, Deguchi S, Yumoto-Sugimoto M, Hanaoka F, Masutani C
    • Journal Title

      PLoS One

      Volume: 10 Issue: 2 Pages: e0118775-e0118775

    • DOI

      10.1371/journal.pone.0118775

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] UV-induced mutations in epidermal cells of mice defective in DNA polymerase η and/or ι2015

    • Author(s)
      Kanao R, Yokoi M, Ohkumo T, Sakurai Y, Dotsu K, Kura S, Nakatsu Y, Tsuzuki T, Masutani C, Hanaoka F
    • Journal Title

      DNA Repair (Amsterdam)

      Volume: S1568-7864(15) Pages: 00042-7

    • DOI

      10.1016/j.dnarep.2015.02.006

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Guanine-5-carboxylcytosine base pairs mimic mismatches during DNA replication.2014

    • Author(s)
      Shibutani T, Ito S, Toda M, Kanao R, Collins LB, Shibata M, Urabe M, Koseki H, Masuda Y, Swenberg JA, Masutani C, Hanaoka F, Iwai S, Kuraoka I
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 5220-5220

    • DOI

      10.1038/srep05220

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] PCNAの翻訳後修飾が制御するDNA損傷トレランス機構2017

    • Author(s)
      金尾梨絵、 増田雄司、 益谷央豪
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] PCNAの翻訳後修飾が制御するDNA損傷トレランスの解析2016

    • Author(s)
      金尾梨絵、 増田雄司、 益谷央豪
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Suppression of PCNA ubiquitination and oxidative stress-induced mutagenesis by USP72016

    • Author(s)
      Kanao R, Kashiwaba S, Masuda Y, Kusumoto-Matsuo R, Hanaoka F, Masutani C
    • Organizer
      The 10th 3R (Replication, Recombination and Repair) symposium
    • Place of Presentation
      Matsue
    • Year and Date
      2016-11-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PCNAのモノユビキチン化の制御によるヒト細胞での酸化的DNA損傷誘発突然変異抑制機構2016

    • Author(s)
      金尾梨絵、柏葉脩一郎、増田雄司、松尾(楠本)理加、花岡文雄、益谷央豪
    • Organizer
      日本薬学会 第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] PCNAのユビキチン化を制御して過酸化水素誘発突然変異を抑制するヒト細胞のメカニズム2015

    • Author(s)
      益谷央豪、柏葉脩一郎、金尾梨絵、松尾(楠本)理加、花岡文雄、増田雄司
    • Organizer
      第38回日本分子生物学会年会第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] PCNAとヒトDNAポリメラーゼηの相互作用2015

    • Author(s)
      増田雄司、金尾梨絵、益谷央豪
    • Organizer
      日本環境変異原学会第44回大会
    • Place of Presentation
      九州大学
    • Year and Date
      2015-11-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 酸化損傷によって誘導されるPCNAのモノユビキチン化を制御する新規メカニズムの解析2015

    • Author(s)
      金尾梨絵、柏葉脩一郎、増田雄司、松尾(楠本)理加、花岡文雄、益谷央豪
    • Organizer
      第23回DNA複製・組換え・修復ワークショップ
    • Place of Presentation
      焼津
    • Year and Date
      2015-10-19
    • Related Report
      2015 Research-status Report
  • [Presentation] PCNAによるヒトDNAポリメラーゼηの活性促進2015

    • Author(s)
      増田雄司、金尾梨絵、益谷央豪
    • Organizer
      第23回DNA複製・組換え・修復ワークショップ
    • Place of Presentation
      焼津
    • Year and Date
      2015-10-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Regulation of DNA damage tolerance by simultaneous mono-ubiquitinations of multiple-units of PCNA in human cells.2015

    • Author(s)
      Kanao R, Masuda Y, Hanaoka F, Masutani C
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] PCNAによるヒトDNAポリメラーゼηの活性促進機構2015

    • Author(s)
      増田雄司、金尾梨絵、益谷央豪
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-24
    • Related Report
      2015 Research-status Report
  • [Presentation] Disruption of Simultaneous Mono-ubiquitinations of Multiple Units of PCNA Homo-trimers Disturbs DNA Damage Tolerance in Human Cells.2015

    • Author(s)
      Masutani C, Kanao R, Masuda Y, Hanaoka F
    • Organizer
      ICRR 2015 15th International Congress of Radiation Resarch
    • Place of Presentation
      京都
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒト細胞におけるPCNAの翻訳後修飾によるDNA損傷トレランス制御機構の解析2015

    • Author(s)
      金尾梨絵, 増田雄司, 花岡文雄, 益谷央豪
    • Organizer
      平成26年度「がん研究分野の特性等を踏まえた支援活動」公開シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2015-01-27 – 2015-01-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 変異体PCNA発現ヒト細胞を用いたDNA損傷トレランス制御の解析2014

    • Author(s)
      金尾梨絵, 増田雄司, 花岡文雄, 益谷央豪
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Relevance of simultaneous mono-ubiquitinations of multiple units of PCNA homo-trimers in DNA damage tolerance2014

    • Author(s)
      Masutani C, Kanao R, Masuda Y, Hanaoka F
    • Organizer
      The 9th 3R (Replication, Recombination and Repair) Symposium
    • Place of Presentation
      御殿場
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Relevance of simultaneous mono-ubiquitinations of multiple units of PCNA homo-trimers in DNA damage torelance2014

    • Author(s)
      Masutani C, Kanao R, Masuda Y, Hanaoka F
    • Organizer
      US-Japan DNA Repair Meeting (日米修復会議)
    • Place of Presentation
      徳島
    • Year and Date
      2014-10-28 – 2014-10-31
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] PCNAの翻訳後修飾によるDNA損傷トレランス制御機構の解析2014

    • Author(s)
      益谷央豪, 金尾梨絵, 増田雄司, 花岡文雄
    • Organizer
      日本放射線影響学会第57回大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2018-03-22  

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