| Project/Area Number |
26740018
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
Sasanuma Hiroyuki 京都大学, 医学(系)研究科(研究院), 准教授 (00531691)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | DNA損傷 / プロテオミクス / ゲノム編集 / 相同組換え / DNA二重鎖切断修復 / プロテオーム / 遺伝学 |
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| Outline of Final Research Achievements |
During the period, I have already established the protocol of mass-spectrometry analysis using DT40 and human Tk6 B cell lines. Initially, I introduced 3flag tag at N-terminal region at first exon of BRCA1 using CRISPR/Cas9 genome editing. After pulling down 3FLAG-BRCA1 from whole cell extract, I analyzed the interactors of BRCA1, which plays a important role in DNA damage repair. I successfully identified many facotrs involved in transcription and chromosome segregation. Additionally, I identified the several novel proteins interacting with BRCA1. To investigate the function of novel BRCA1-interactors in DNA metabolism (DNA repair, replication, recombination), I continue generating the mutants of BRCA1 interactors identified here using human B cell line by genome editing technology. It is thought that the aim of this project was achieved.
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