Analysis of a novel regulatory mechanism of DNA damage repair using the combination of proteomics and genetics approarch
Project/Area Number |
26740018
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Risk sciences of radiation and chemicals
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Research Institution | Kyoto University |
Principal Investigator |
Sasanuma Hiroyuki 京都大学, 医学(系)研究科(研究院), 准教授 (00531691)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | DNA損傷 / プロテオミクス / ゲノム編集 / 相同組換え / DNA二重鎖切断修復 / プロテオーム / 遺伝学 |
Outline of Final Research Achievements |
During the period, I have already established the protocol of mass-spectrometry analysis using DT40 and human Tk6 B cell lines. Initially, I introduced 3flag tag at N-terminal region at first exon of BRCA1 using CRISPR/Cas9 genome editing. After pulling down 3FLAG-BRCA1 from whole cell extract, I analyzed the interactors of BRCA1, which plays a important role in DNA damage repair. I successfully identified many facotrs involved in transcription and chromosome segregation. Additionally, I identified the several novel proteins interacting with BRCA1. To investigate the function of novel BRCA1-interactors in DNA metabolism (DNA repair, replication, recombination), I continue generating the mutants of BRCA1 interactors identified here using human B cell line by genome editing technology. It is thought that the aim of this project was achieved.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] The Double-Strand Break Landscape of Meiotic Chromosomes Is Shaped by the Paf1 Transcription Elongation Complex in Saccharomyces cerevisiae2016
Author(s)
Gothwal, S.K., Patel, N.J., Colletti, M.M., Sasanuma, H., Shinohara, M., Hochwagen, A., and Shinohara, A
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Journal Title
Genetics
Volume: 202
Pages: 497-512
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Abacavir, an anti-HIV-1 drug, targets TDP1-deficient adult T cell leukemia2015
Author(s)
Tada, K., Kobayashi, M*., et al, Sasanuma, H, Takeda, S, Takaori-K, A
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Journal Title
Science advances
Volume: 1
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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