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Development and analysis of environmental friendly and energy saving lactic acid production process by thermophilic Bacillus sp.

Research Project

Project/Area Number 26740050
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Design and evaluation of sustainable and environmental conscious system
Research InstitutionKyushu University

Principal Investigator

Tashiro Yukihiro  九州大学, (連合)農学研究科(研究院), 助教 (90448481)

Co-Investigator(Renkei-kenkyūsha) SAKAI Kenji  九州大学, 大学院農学研究院, 教授 (50205704)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsデンプン / Bacillus sp. MC-07株 / 直接高温乳酸発酵 / L-乳酸光学純度 / 好熱性菌 / バチルス属細菌 / L-乳酸 / 直接発酵プロセス
Outline of Final Research Achievements

New thermophilic L-lactic acid producing Bacillus sp. MC-07 strain with starch utilization was isolated and identified. This strain was different from Bacillus thermoamylovorans LMG 18084T at species level regardless of 99.2% similarity of 16S rRNA gene sequence. This strain showed higher lactic acid production by pH-swing control every 12 h using gelatinized starch with heat treatment than LMG 18084T strain. Furthermore, we could establish a direct lactic acid production process without saccharification process from non-gelatinized starch.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (10 results) (of which Invited: 2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Pramod Poudel, Yukihiro Tashiro, Kenji Sakai2016

    • Author(s)
      New application of Bacillus strains for optically pure L-lactic acid production: general overview and future prospects
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 80 Issue: 4 Pages: 642-654

    • DOI

      10.1080/09168451.2015.1095069

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Direct starch fermentation to L-lactic acid by a newly isolated thermophilic strain, Bacillus sp. MC-072015

    • Author(s)
      Pramod Poudel、田代幸寛、宮本浩邦、宮本久、奥川友紀、酒井謙二
    • Journal Title

      Journal of Industrial Microbiology & Biotechnology

      Volume: 42 Issue: 1 Pages: 143-149

    • DOI

      10.1007/s10295-014-1534-0

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Characterization of a novel bacterium species isolated from thermophilic compost prepared from marine animal resources material2016

    • Author(s)
      Clament Chin Fui Seung, Pramod Poudel, Hirokuni Miyamoto, Yuki Okugawa, Yukihiro Tashiro, Kenji Sakai
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 複合微生物系を用いたメタ発酵による有価物変換法の制御と体系化2016

    • Author(s)
      田代幸寛、酒井謙二
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 無蒸煮デンプンの直接高温乳酸発酵に関する研究2015

    • Author(s)
      石橋侑子, Pramod Poudel, 奥川友紀, 田代幸寛, 宮本浩邦, 酒井 謙二
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] L-乳酸の生産のための混合培養系の構築2015

    • Author(s)
      古原俊哉、プラモド ポウデル、奥川友紀、田代幸寛、酒井謙二
    • Organizer
      第52回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場(福岡)
    • Year and Date
      2015-06-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 複合微生物系による有価物発酵生産(メタ発酵)における発酵諸条件の影響2015

    • Author(s)
      田代幸寛、井ノ口翔太、石橋侑子、宮本浩邦、奥川友紀、酒井謙二
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Mixed culture system for sustainable utilization of food waste biomass to produce value added products under high temperature2014

    • Author(s)
      Pramod Poudel, 田代幸寛, 酒井謙二,
    • Organizer
      The 8th International Symposium on the East Asian Environmental Problems (EAEP2014)
    • Place of Presentation
      Fukuoka (Japan)
    • Year and Date
      2014-12-09 – 2014-12-10
    • Related Report
      2014 Research-status Report
  • [Presentation] スクロースを炭素源とした高温L-乳酸発酵における生ゴミ中の発酵促進因子2014

    • Author(s)
      鷹川恵利、藤崎紗織、奥川友紀、田代幸寛、酒井謙二
    • Organizer
      第21回日本生物工学会九州支部大会(2014)
    • Place of Presentation
      熊本大学
    • Year and Date
      2014-12-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Development of total recycle system of food waste biomass to produce value added products under thermophilic conditions2014

    • Author(s)
      Pramod Poudel, 田代幸寛, 酒井謙二,
    • Organizer
      International Scientific Conference in Environmental Research
    • Place of Presentation
      Lumbini (Nepal)
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Research-status Report
  • [Presentation] 複合微生物系を活用したメタ発酵における発酵諸条件の影響2014

    • Author(s)
      井ノ口翔太、石橋侑子、田代幸寛、酒井謙二
    • Organizer
      2014年度日本農芸化学会西日本支部大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-18 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 複合微生物系を用いたメタ発酵に及ぼす種菌と温度の影響2014

    • Author(s)
      石田勇貴、井ノ口翔太、田代幸寛、酒井謙二
    • Organizer
      2014年度日本農芸化学会西日本支部大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-18 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Remarks] 九州大学大学院農学研究院土壌環境微生物学研究室

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/dobi/

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] デンプンから生分解性プラスチック原料のL型乳酸を高効率に生産可能な好熱菌資材、並びにL型乳酸の製造方法2014

    • Inventor(s)
      酒井謙二、田代幸寛、プラマド ポウデル、宮本浩邦
    • Industrial Property Rights Holder
      酒井謙二、田代幸寛、プラマド ポウデル、宮本浩邦
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-194405
    • Filing Date
      2014-09-04
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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