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Regulation of the staphylococcal enterotoxin produced by Staphylococcus aureus in cooked rice

Research Project

Project/Area Number 26750021
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionNiigata University (2017)
The University of Tokyo (2016)
Ochanomizu University (2014-2015)

Principal Investigator

TSUTSUURA Satomi  新潟大学, 研究推進機構, 特任助教 (20708622)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords黄色ブドウ球菌 / エンテロトキシンA (SEA) / 毒素 / 米飯 / Western blot / グリシン / 食塩 / pH / エンテロトキシンA(SEA) / エンテロトキシン / 塩分濃度 / 初発菌数
Outline of Final Research Achievements

To examine the effect of various factors on the growth of Staphylococcus aureus and the production of staphylococcal enterotoxin A (SEA) in cooked rice, we inoculated strains of SEA-producing S. aureus into cooked rice, and then quantified the bacterial number and SEA after incubating them at 37oC. Most strains produced less SEA in cooked rice added with 2-5% glycine than in the non-added control. Some strains produced less SEA in cooked rice added with 5-7% NaCl than in the non-added control. The inhibition of growth of S. aureus and SEA production was not apparent when NaCl was added to cooked rice at the presence of glycine. Most strains produced less SEA in cooked rice adjusted pH 4.0-4.5 than in the non-adjusted control. It is necessary to further examine the combination of these inhibition methods for the inhibition of SEA production in cooked rice while preserving good taste of cooked rice.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2018 2017 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Production of staphylococcal enterotoxin A in cooked rice and limitation of its organoleptic detection2017

    • Author(s)
      Satomi Tsutsuura and Masatsune Murata
    • Journal Title

      Food Science Technology Reaserch

      Volume: 23 Pages: 267-274

    • NAID

      130005681788

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 米飯保存時の黄色ブドウ球菌staphylococcal enterotoxin A(SEA)産生に与えるpH及びグリシンの影響についての菌株間における比較2018

    • Author(s)
      筒浦さとみ、村田容常
    • Organizer
      日本家政学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 黄色ブドウ球菌のエンテロトキシン産生を制御するための米飯の保存方法の検討2017

    • Author(s)
      筒浦さとみ、村田容常
    • Organizer
      日本栄養・食糧学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 米飯へのグリシン添加が黄色ブドウ球菌毒素産生と色及び臭いに与える影響2016

    • Author(s)
      筒浦さとみ、村田容常
    • Organizer
      日本食品科学工学会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2016-08-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 米飯におけるエンテロトキシンA産生とその制御2016

    • Author(s)
      筒浦さとみ、 村田容常
    • Organizer
      日本農芸化学会
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 米飯による黄色ブドウ球菌食中毒の予防に対する感覚的検知の限界2015

    • Author(s)
      筒浦さとみ、 村田容常
    • Organizer
      日本農芸化学会 関東支部大会
    • Place of Presentation
      お茶の水女子大学(東京)
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Enterotoxin A production by Staphylococcus aureus in a liquid medium and cooked rice2015

    • Author(s)
      Satomi Tsutsuura and Masatsune Murata
    • Organizer
      ACN2015
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2015-05-15 – 2015-05-17
    • Related Report
      2014 Research-status Report
  • [Presentation] Enterotoxin A production by Staphylococcus aureus in a liquid medium and cooked rice2015

    • Author(s)
      Satomi Tsutsuura and Masatsune Murata
    • Organizer
      12 th Asian Congress of Nurtrition
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2015-05-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 保存米飯中におけるエンテロトキシンAの検出ならびに黄色ブドウ球菌の増殖とエンテロトキシンA産生の関係について2014

    • Author(s)
      筒浦さとみ, 村田容常
    • Organizer
      食品分析研究会
    • Place of Presentation
      東洋大学白山キャンパス
    • Year and Date
      2014-09-05
    • Related Report
      2014 Research-status Report
  • [Presentation] 米飯保存時のstaphylococcal enterotoxin A(SEA)産生に与える影響についての黄色ブドウ球菌菌株間における比較2014

    • Author(s)
      筒浦さとみ, 林田直子, 村田容常
    • Organizer
      日本食品科学工学会
    • Place of Presentation
      中村学園大学
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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