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Characterization and gene expression analysis of enzymes from a water extract of Ganoderma lucidum

Research Project

Project/Area Number 26750024
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionTakasaki University of Health and Welfare

Principal Investigator

Kumakura Kei  高崎健康福祉大学, 健康福祉学部, 助教 (80516930)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsきのこ / 酵素 / 遺伝子 / プロテアーゼ / グルカナーゼ / マンネンタケ / 遺伝子発現 / グルコシダーゼ
Outline of Final Research Achievements

This study focused on enzymes of a Ganoderma lucidum water extract. These enzymes studied were glutamic acid protease (GlGPA) and β-1,3-glucanase (GlGH128A). Full-length cDNA sequences of GlGPA and GlGH128A were obtained by using rapid amplification of cDNA ends (RACE)-PCR. Further, gene expression of GlGPA and GlGH128A was analyzed during growth stages and the harvest storage period. It was revealed that gene expression of GlGPA increased in mycelium stage, primordium of fruiting body stage, fruiting body before ripening stage, and fruiting body of 4 weeks after harvesting. On the other hand, gene expression of GlGH128A increased in fruiting bodies of 1, 2, and 4 weeks after harvesting. These results suggest that GlGPA and GlGH128A are important for morphogenesis and aging of bruiting body.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (3 results)

All 2016 2015 Other

All Presentation (2 results) Remarks (1 results)

  • [Presentation] マンネンタケ(Ganoderma lucidum)子実体からのβ-1,3-グルカナーゼ遺伝子のクローニング2016

    • Author(s)
      熊倉 慧、斎藤豪仁、石田勇輝、永井俊匡、松岡寛樹、堀 千明、石田卓也、 五十嵐圭日子、鮫島正浩
    • Organizer
      日本きのこ学会第20回大会
    • Place of Presentation
      静岡県静岡市駿河区馬渕1丁目17-1静岡県男女共同参画センター「あざれあ」
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] マンネンタケ(Ganoderma lucidum)菌糸体からのグルタミン酸プロテアーゼ遺伝子のクローニング2015

    • Author(s)
      熊倉 慧、永井俊匡、松岡寛樹、堀 千明、五十嵐圭日子、鮫島正浩
    • Organizer
      日本きのこ学会第19回大会
    • Place of Presentation
      茨城県つくば市竹園2丁目20-3 つくば国際会議場
    • Year and Date
      2015-09-04
    • Related Report
      2015 Research-status Report
  • [Remarks] 高崎健康福祉大学 健康福祉学部 健康栄養学科 教員紹介 熊倉慧

    • URL

      http://www.takasaki-u.ac.jp/p_eiyo/1491/

    • Related Report
      2016 Annual Research Report 2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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