| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The skin of peanut, Arachis hypogaea L., is a rich source of polyphenols. We identified Procyanidin A1 as a dimer and epicatechin-(4β→6)-epicatechin-(2β→O→7, 4β→8)-catechin (EEC) as a procyanidin trimer from the skin of peanut. EEC showed strong inhibitory activities of alfa-amylase, maltase, sucrase. EEC also decreased the absorption of glucose from the intestine by inhibition of glucose transport.
On the other hands, EEC has antimicrobial activity, in particular food-borne gram-positive bacteria, Bacillus cereus. DNA microarray analysis of B. cereus treated with EEC was carried out, with a finding that 597 genes were significantly up-regulated. Analysis of the up-regulated genes suggested that EEC disrupted the normal condition of the cell membrane and wall of B. cereus and alter its usual nutritional metabolism. Moreover, treatment of B. cereus with EEC inhibited glucose uptake, suggesting that EEC affects the cell-surface adsorption.
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