| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We performed a qualitative analysis and assay of free radicals derived from peroxides within heated vegetable oil, using the ESR spin trapping method. The spontaneous radical generation method that has been used in previous studies exhibited poor reproducibility and provided only weak ESR signals. We applied UV-light illumination to forcefully generate free radicals from the oxidized oil. The spin trap PBN was adopted as a free-radical trapping agent to detect free-radical adducts. With a strictly controlled UV-illumination procedure, we discovered that the free radical concentration increased as the heat-treatment time of the vegetable oil was extended, i.e., in proportion to the progress of oxidation. The analysis of ESR spectra led to the conclusion that the free radicals showing ESR signals in vegetable oils were alkyl peroxyl and alkoxyl radical. We included that we established the method to evaluate oxidation levels for vegetable oil using ESR spin trap method.
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