| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Outline of Final Research Achievements |
n-3 polyunsaturated fatty acids (PUFA) can reduce plasma triglyceride (TG) level. However, the underlying mechanism of plasma TG reduction remains unclear. Carbohydrate response element-binding protein (ChREBP) is a transcription factor responsible for the coordinated metabolism of carbohydrate and fat synthesis. It was reported that the inhibition of ChREBP in leptin-deficient mice improved plasma TG level. Therefore, it was suggested that ChREBP could be a protein affected by n-3 PUFA, which was involved in reducing plasma TG level. In this study, it was shown that n-3 PUFA could promote the degradation of ChREBP. Moreover, it was found that ChREBP was degraded via the autophagy-lysosomal pathway. The inhibitory effect on ChREBP activity by n-3 PUFA disappeared when the degradation of ChREBP was inhibited by chloroquine treatment. These results suggested that n-3 PUFA promoted the degradation of ChREBP by inducing autophagy, resulting in reduction of plasma TG level.
|
