| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Human α-defensin (HD) 5 was antimicrobial peptide secreted as components of the innate immune defense system. Although intestinal immunity peptides are considered to be important in intestinal epithelial barrier function, it has not been studied the relationships between HD 5 and intestinal transporters involved in the barrier for orally absorbed drugs. We performed to determine the effects of HD 5 on intestinal transporters in Caco-2 cells.
Firstly, we examined the effect of HD 5 on the expression of intestinal transporters. The results showed that HD 5 treatment was reduced expression of MRP2 and MDR1 in Caco-2 cells. Moreover, we examined the effects of treatment with HD5 knockdown on MRP2 and MDR1 expression in Caco-2 cells. The results showed MRP2 and MDR1 mRNA expression was induced by HD5 knockdown. These results suggest that MDR1 and MRP2 are regulated by HD 5.
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