Synthesis of aligned megadalton hemoglobin assembly as artificial oxygen carrier

Research Project

Project/Area Number	26750149
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Biomedical engineering/Biomaterial science and engineering
Research Institution	Nara Medical University
Principal Investigator	Matsuhira Takashi 奈良県立医科大学, 医学部, 助教 (20570998)
Research Collaborator	SAKAI Hiromi
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	人工臓器学 / 血液代替物 / 高分子 / ヘモグロビン
Outline of Final Research Achievements	There have been many attempts to make artificial oxygen carriers based on the modification of isolated hemoglobin (Hb). In these attempts, artificial oxygen carrier realizes improved characteristics, avoiding infection, no blood type, and longer shelf life than red blood cells (RBC). In this work, novel large-sized Hb assembly (aligned megadalton Hb; AM-Hb) was synthesized by intermolecular crosslinking of Hbs with 4-armed polyethylene glycol (PEG) to prevent extravasation. The physicochemical properties as oxygen carrier was evaluated for AM-Hb. The molecular size of AM-Hb depends on the molecular weight of PEG linker. AM-Hb has larger viscosity and larger oxygen affinity than those of RBC, Hb, and Hb vesicles.