Neuroregeneration by concerted regulation of cellular microenvironments

• Project/Area Number: 26750156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Hashimoto Yoshihide 東京医科歯科大学, 生体材料工学研究所, 助教 (40638384)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ナノゲル / 細胞微小環境 / 神経再生 / グリコサミノグリカン / 組織工学 / エキソソーム / 多糖 / 成長因子 / ナノマトリクス

Outline of Final Research Achievements

The purpose of this study is to develop the nano-matrix that mimicked cellular microenvironments for regeneration of injured neural tissues. We established the synthetic method of methacryloyl group- and cholesteryl group- bearing chondroitin sulfate (CH-GAG-MA), and successfully developed novel nanogels. We also developed the nano-matrix complexed with proteins and exosomes using CH-GAG-MA nanogels as building blocks.

Research Products

• [Journal Article] Advanced artificial extracellular matrices using amphiphilic nanogel-cross-linked thin films to anchor adhesion proteins and cytokines (2016)
  • Author(s): Y. Hashimoto, S. Mukai, S. Sawada, T. Sasaki, K. Akiyoshi
  • Journal Title: ACS Biomaterials Science & Engineering Volume: 2 Issue: 3 Pages: 375-384
  • DOI: 10.1021/acsbiomaterials.5b00485
  • Peer Reviewed
• [Journal Article] Effects of a polysaccharide nanogel-crosslinked membraneon wound healing (2016)
  • Author(s): Hiroki Maeda,Hiroshi Kobayashi,Takayuki Miyahara,Yoshihide Hashimoto,Kazunari Akiyoshi,Shohei Kasugai
  • Journal Title: Jouranal of Biomedical Materials research Part B Applied Materials Volume: 未定 Issue: 3 Pages: 554-560
  • DOI: 10.1002/jbm.b.33571
  • Peer Reviewed
• [Journal Article] Nanogel tectonic porous gel loading biologics, nanocarriers, and cells for advanced scaffold (2015)
  • Author(s): Y. Hashimoto, S. Mukai,S. Sawada, Y. Sasaki, K. Akiyoshi
  • Journal Title: Biomaterials Volume: 37 Pages: 107-115
  • DOI: 10.1016/j.biomaterials.2014.10.045
  • Peer Reviewed
• [Journal Article] Cycloamylose-Nanogel DDS-mediated intratumor silencing of the vascular endothelial growth factor regulates neovascularization in tumor microenvironment (2014)
  • Author(s): H. Fujii, M. Shin-Ya, S. Takeda, Y. Hashimoto, S. Mukai, S. Sawada, T. Adachi, K. Akiyoshi, T. Miki, O. Mazda
  • Journal Title: Cancer Science Volume: 105 Issue: 12 Pages: 1616-1625
  • DOI: 10.1111/cas.12547
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Therapeutic Effect of Nanogel-Based Delivery of Soluble FGFR2 with S252W Mutation on Craniosynostosis (2014)
  • Author(s): M. Yokota, Y. Kobayashi, J. Morita, H. Suzuki, Y. Hashimoto, Y. Sasaki, K. Akiyoshi, K. Moriyama
  • Journal Title: PLOS ONE Volume: 9 Issue: 7 Pages: e101693-e101693
  • DOI: 10.1371/journal.pone.0101693
  • Peer Reviewed / Open Access
• [Presentation] 有機-無機ハイブリッドポーラス材料の設計と再生医療応用 (2015)
  • Author(s): 藤原理絵, 橋本良秀, 丸川恵理子, 澤田晋一, 向井貞篤, 佐々木善浩, 秋吉一成
  • Organizer: 第37回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ（京都府, 京都市）
  • Year and Date: 2015-11-09
• [Presentation] 有機-無機ハイブリッドポーラス材料の設計と医療応用 (2015)
  • Author(s): 藤原理絵, 橋本良秀, 丸川恵理子, 澤田晋一, 向井貞篤, 佐々木善浩, 秋吉一成
  • Organizer: 第64回高分子討論会
  • Place of Presentation: 東北大学川内キャンパス（宮城県, 仙台市）
  • Year and Date: 2015-09-15
• [Presentation] ナノゲルテクトニクス材料：ナノゲル架橋ハイブリッド フィルムの創製と機能評価 (2015)
  • Author(s): 向井貞篤, 橋本良秀, 田原義朗, 澤田晋一, 秋吉一成
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター（北海道, 札幌市）
  • Year and Date: 2015-05-27
• [Presentation] 多糖ナノボールの集積制御に基づく新規ゲル材料の設計とバイオマテリアル応用 (2015)
  • Author(s): 沖田圭司, 竹田茂生, 橋本良秀, 向井貞篤, 澤田晋一, 佐々木善浩, 秋吉一成
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター（北海道, 札幌市）
  • Year and Date: 2015-05-27
• [Presentation] 多孔質構造を有するナノゲル架橋ハイブリッドゲルの開発と機能評価 (2014)
  • Author(s): 橋本良秀、向井貞篤、澤田晋一、秋吉一成
  • Organizer: 第63回高分子討論会
  • Place of Presentation: 長崎
  • Year and Date: 2014-09-24 – 2014-09-26
• [Presentation] Fabrication of nanogel-crosslinked porous gels for tissue engineering applications (2014)
  • Author(s): Yoshihide Hashimoto, Sada-atsu Mukai, Shin-ichi Sawada, Kazunari Akiyoshi
  • Organizer: The 41st Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Chicago, USA
  • Year and Date: 2014-07-13 – 2014-07-16
• [Remarks] 京都大学 秋吉研究室
  • URL: http://www.akiyoshi-lab.jp/
• [Remarks] ERATO秋吉プロジェクト
  • URL: http://www.jst.go.jp/erato/akiyoshi/index.html