| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Cells become “endoplasmic reticulum (ER) stress” under the accumulation of abnormal proteins inside ER and induce apoptosis under the excess ER stress. In this study, ER stress derived cell apoptosis inducing system was developed by the intracellular delivery of protein reactive molecules using nanoparticles to attack proteins inside cells. To attack intracellular proteins, the study employed chloromethyl compounds because chloromethyl chemicals have high reactivity with thiol groups on proteins. The study revealed that several chloromethyl compounds, in particular chloromethyl alkenes, showed scarce cellular cytotoxicity when they applied to cells alone. In contrast, chloromethyl compounds exhibited high cytotoxicity by intracellular introduction with nanoparticles. From the results, chloromethyl compounds are possibly applied to new cell death inducing agents by using them with nanopartilces that possess selective intracellular uptake property.
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