Development of a cytoplasmic RNA vector to modulate microRNA function for cellular reprogramming
| Project/Area Number |
26750163
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SANO Masayuki 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (80415687)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | マイクロRNA / RNAベクター / 細胞改変技術 / 遺伝子導入 / 機能制御 / 遺伝子発現 |
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| Outline of Final Research Achievements |
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by binding to the 3’ unltanslated region of target mRNAs. It has been demonstrated that several tissue-specific miRNAs can modulate cellular reprogramming. In this study, we tried to construct a cytoplasmic RNA vector, based on Sendai virus, which not only produces miRNAs but also inhibits the miRNA activity, so as to apply in cellular reprogramming.
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Report
(3 results)
Research Products
(4 results)
