Exercise-induced mitochondrial biogenesis and DNA methylation

• Project/Area Number: 26750304
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Sports science
• Research Institution: The University of Tokyo
• Principal Investigator: KITAOKA Yu 東京大学, 総合文化研究科, 助教 (30726914)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 運動 / 骨格筋 / ミトコンドリア

Outline of Final Research Achievements

It is well known that exercise induces mitochondrial biogenesis in skeletal muscle. The aim of our study was to examine the effect of exercise on DNA methylation. DNMT1 (DNA methyltransferase 1) protein content was not altered after exercise training, but significantly decreased after denervation in skeletal muscle. Our observations suggest that inactivity-induced muscle dysfunction may be induced by increased DNA methylation levels.

Research Products

• [Journal Article] Lactate administration increases mRNA expression of PGC-1α and UCP3 in mouse skeletal muscle (2016)
  • Author(s): Yu Kitaoka, Kohei Takeda, Yuki Tamura, Hideo Hatta
  • Journal Title: Applied Physiology, Nutrition, and Metabolism Volume: - Issue: 6 Pages: 695-698
  • DOI: 10.1139/apnm-2016-0016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of electrical stimulation-induced resistance exercise on mitochondrial fission and fusion proteins in rat skeletal muscle (2015)
  • Author(s): Yu Kitaoka, Riki Ogasawara, Yuki Tamura, Satoshi Fujita, Hideo Hatta
  • Journal Title: Applied Physiology, Nutrition, and Metabolism Volume: 40 Issue: 11 Pages: 1137-1142
  • DOI: 10.1139/apnm-2015-0184
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Lactate: a signaling molecule for training adaptation (2016)
  • Author(s): Yu Kitaoka
  • Organizer: Ergogenic Aids and Nutritional Supplements for Health and Sports 2016
  • Place of Presentation: コンケン（タイ）
  • Year and Date: 2016-01-20
  • Int'l Joint Research / Invited
• [Presentation] Effect of resistance exercise on mitochondrial fission and fusion proteins in rat skeletal muscle (2015)
  • Author(s): Yu Kitaoka, Riki Ogasawara, Yuki Tamura, Satoshi Fujita, Hideo Hatta
  • Organizer: 第70回日本体力医学会大会（国際セッション）
  • Place of Presentation: 和歌山県民文化会館（和歌山県和歌山市）
  • Year and Date: 2015-09-18
• [Presentation] レジスタンストレーニングによる骨格筋ミトコンドリア 分裂・融合タンパク質量の変化 (2015)
  • Author(s): 北岡祐, 小笠原理紀, 田村優樹, 八田秀雄
  • Organizer: 第23回日本運動生理学会大会
  • Place of Presentation: 日本体育大学（東京都世田谷区）
  • Year and Date: 2015-07-25
• [Presentation] Resistance Exercise and Mitochondrial Dynamics (2015)
  • Author(s): Yu Kitaoka, Riki Ogasawara, Yuki Tamura, Satoshi Fujita, Hideo Hatta
  • Organizer: Cell Symposia: Exercise Metabolism
  • Place of Presentation: アムステルダム（オランダ）
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Presentation] Whole body heat stress increases muscle PGC-1alpha expression in C57BL/6J mice (2014)
  • Author(s): Yu Kitaoka, Mats I. Nilsson, Bart P. Hettinga, Olivia S. Marquez, Rahul Suri, and Mark A. Tarnopolsky
  • Organizer: ACSM Conference on Integrative Physiology of Exercise
  • Place of Presentation: Eden Roc Miami Beach (Florida, USA)
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] 運動時の乳酸代謝～グリコーゲン代謝の遺伝性疾患からサラブレッドまで～ (2014)
  • Author(s): 北岡祐
  • Organizer: 日本体育学会第65回大会
  • Place of Presentation: 岩手大学（岩手県盛岡市）
  • Year and Date: 2014-08-25 – 2014-08-28