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Novel method for live cell actin imaging based on small molecule fluorescent probe

Research Project

Project/Area Number 26750369
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemical biology
Research InstitutionThe University of Tokyo

Principal Investigator

Ueno Tasuku  東京大学, 大学院薬学系研究科(薬学部), 助教 (60462660)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords蛍光プローブ / アクチン / イメージング / アクチン骨格 / 生細胞イメージング / 有機小分子 / アクチン繊維 / 構造活性相関
Outline of Final Research Achievements

The dynamic behavior of the actin cytoskeleton is a critical part of most cellular activities, e.g., membrane trafficking, cell movement and neurite extension. Therefore, visualization of actin cytoskeleton dynamics is essential to understand its roles in regulating cellular functions. In the present study, novel small molecule based fluorescence probe for F-actin visualization probes were successfully developed. The developed probe is simple to use, and has sufficiently high sensitivity and specificity for actin cytoskelton. It is also showed that the probe was applicable for live cell fluorescence imaging method. This would be nice piece of work a potentially important to researchers interested in the biology associated with actin dynamics.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 2015

All Presentation (5 results)

  • [Presentation] 生細胞におけるアクチン骨格の制御・可視化による機能の理解2017

    • Author(s)
      上野匡
    • Organizer
      第一回公開シンポジウム「数理シグナル」学術領域の創出
    • Place of Presentation
      東京大学医科学研究所(東京都 港区)
    • Year and Date
      2017-02-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] アクチン骨格の制御・可視化による細胞機能の理解2016

    • Author(s)
      上野匡
    • Organizer
      第9回ナノバイオ若手ネットワーキングシンポジウム
    • Place of Presentation
      東北大学(宮城県 仙台市)
    • Year and Date
      2016-06-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] アクチン繊維に高い親和性を有する新規低分子化合物2015

    • Author(s)
      上野匡,野村悠介,浅沼大祐,浦野泰照
    • Organizer
      生体機能関連化学部会若手の会 第27回サマースクール
    • Place of Presentation
      神戸セミナーハウス (神戸市北区道場町)
    • Year and Date
      2015-07-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 小分子蛍光プローブによるアクチンフィラメントの可視化2015

    • Author(s)
      野村悠介,上野匡,浅沼大祐,浦野泰照
    • Organizer
      第15回東京大学生命科学シンポジウム
    • Place of Presentation
      東京大学 (東京都文京区)
    • Year and Date
      2015-06-27
    • Related Report
      2015 Research-status Report
  • [Presentation] 小分子蛍光プローブによるアクチン繊維の可視化2015

    • Author(s)
      野村悠介、上野匡、浅沼大祐、長野哲雄、浦野泰照
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      兵庫県(神戸市) 神戸学院大学
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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