Research Project
Grant-in-Aid for Young Scientists (B)
In this study, fluorescent probes were developed for detecting histone deacetylase (HDAC) activity involved in epigenetic gene expression. We designed and synthesized peptide conjugates between nuclear staining dye and HDAC substrates based on the control of DNA interaction upon deacetylation. Reaction of the probes with HDAC in the presence of DNA resulted in fluorescence enhancement in concomitant with the increase of DNA binding affinity. Our method demonstrated rapid detection of HDAC activity with a one-pot procedure.
All 2017 2016 2015 2014
All Journal Article (3 results) (of which Peer Reviewed: 3 results, Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Int'l Joint Research: 2 results)
Journal of Biological Inorganic Chemistry
Volume: - Issue: 5 Pages: 1-14
10.1007/s00775-016-1435-y
Analytical Sciences
Volume: 31 Issue: 4 Pages: 287-292
10.2116/analsci.31.287
130005061530
Anal. Chem.
Volume: 86 Issue: 15 Pages: 7925-7930
10.1021/ac501881s
