Mechanism of oscillation in the hippocampal microcircuit

• Project/Area Number: 26780411
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental psychology
• Research Institution: Jikei University School of Medicine (2015-2017); Hokkaido University (2014)
• Principal Investigator: Suzuki Etsuko 東京慈恵会医科大学, 医学部, 助教 (60424313)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 海馬 / オシレーション / カルバコール / カイニン酸受容体 / シータオシレーション / ガンマオシレーション

Outline of Final Research Achievements

The induction mechanism of theta oscillation and gamma oscillation in the hippocampus was examined. Acute hippocampal slices were obtained from mice or rats, and the acetylcholine receptor agonist carbachol was perfused to induce theta oscillation. Electrophysiological recordings were carried out in hippocampal CA3 pyramidal neurons. Stable theta oscillation was induced by carbachol at a perfusion temperature of 33-35℃. In some cases, transient gamma oscillation was also induced during a theta oscillation cycle. Carbachol-induced gamma oscillation was suppressed by a kainite-type glutamate receptor blocker.

Research Products

• [Journal Article] PSD-95 regulates synaptic kainate receptors at mouse hippocampal mossy fiber-CA3 synapses (2015)
  • Author(s): Suzuki E & Kamiya H
  • Journal Title: Neuroscience Research Volume: x Pages: 1-6
  • DOI: 10.1016/j.neures.2015.12.011
  • NAID: 120006219902
  • Peer Reviewed
• [Journal Article] Cholinergic modulation for hippocampal long-term potentiation (2014)
  • Author(s): Suzuki, E.
  • Journal Title: Japanese Journal of Animal Psychology Volume: 64 Issue: 1 Pages: 37-41
  • DOI: 10.2502/janip.64.1.4
  • NAID: 130004938658
  • ISSN: 0916-8419, 1880-9022
  • Peer Reviewed / Open Access
• [Presentation] Muscarinic inhibition of GABAergic transmission onto striatal cholinergic interneurons involves M1 receptor activation. (2017)
  • Author(s): Suzuki, E. & Momiyama, T.
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松
  • Year and Date: 2017-03-28
• [Presentation] Inhibition of GABAergic transmission onto striatal cholinergic interneurons via muscarine 1 receptor activation. (2017)
  • Author(s): Suzuki, E. & Momiyama, T.
  • Organizer: 第90回日本薬理学会年会
  • Place of Presentation: 長崎
  • Year and Date: 2017-03-15
• [Presentation] Postsynaptic G-protein cascade is not required for the inhibitory cholinergic modulation of GABAergic transmission onto striatal cholinergic interneurons. (2017)
  • Author(s): Etsuko Suzuki & Toshihiko Momiyama
  • Organizer: 日本神経科学学会
• [Presentation] Inhibition of GABAergic transmission onto striatal cholinergic interneurons by M1 receptor activation (2017)
  • Author(s): Etsuko Suzuki & Toshihiko Momiyama
  • Organizer: 行動2017（ 日本動物心理学会・日本動物行動学会・応用動物行動学会・日本家畜管理学会・日本行動神経内分泌研究会合同大会）
• [Presentation] M1 receptor-mediated presynaptic inhibition of GABAergic transmission from striatal medium spiny neurons onto cholinergic interneurons (2017)
  • Author(s): Etsuko Suzuki & Toshihiko Momiyama
  • Organizer: 日本生理学会
• [Presentation] GABAergic transmission onto striatal cholinergic interneurons is inhibited by muscarine receptor activation. (2016)
  • Author(s): Suzuki, E. & Momiyama, T.
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Modulation of GABAergic transmission onto striatal cholinergic interneurons via muscarinic receptors. (2016)
  • Author(s): Suzuki, E. & Momiyama, T.
  • Organizer: 10th FENS forum of European neuroscience
  • Place of Presentation: コペンハーゲン
  • Year and Date: 2016-07-02
• [Presentation] Muscarine receptor-mediated inhibition of GABAergic transmission onto striatal cholinergic interneurons. (2016)
  • Author(s): Suzuki, E & Momiyama, T.
  • Organizer: 日本生理学会第93回大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-22
• [Presentation] Input specificity of giant miniature EPSC in hippocampal CA3 neurons. (2015)
  • Author(s): Suzuki, E. & Kamiya, H.
  • Organizer: 日本生理学会第92回大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県神戸市）
  • Year and Date: 2015-03-21
• [Presentation] Reduced kainate receptors at hippocampal mossy fiber synapse in PSD-95 knockout mice. (2014)
  • Author(s): Suzuki, E. & Kamiya, H.
  • Organizer: Neuroscience2014(Society for Neuroscience)
  • Place of Presentation: Washington D.C. (USA)
  • Year and Date: 2014-11-19
• [Presentation] ニューロンシミュレーターを用いた海馬苔状線維の興奮伝播モデルの改良 (2014)
  • Author(s): 鈴木江津子・神谷温之
  • Organizer: 生理学会北海道地方会
  • Place of Presentation: 北海道大学学術交流会館（北海道札幌市）
  • Year and Date: 2014-08-30
• [Presentation] Suppression of gamma frequency oscillation by a selective blocker of kainate-type glutamate receptor in CA3 region of mouse hippocampus. (2014)
  • Author(s): Suzuki E & Kamiya H.
  • Organizer: 日本動物心理学会第74回大会
  • Place of Presentation: 犬山国際観光センター フロイデ（愛知県犬山市）
  • Year and Date: 2014-07-19