Light Sheet Microscope Technics for a Single Molecule Imaging in Eukaryotic cell
| Project/Area Number |
26790027
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Nanobioscience
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Nishimura Kazuya 国立研究開発法人理化学研究所, 生命システム研究センター, 客員研究員 (50643186)
|
|---|
| Research Collaborator |
TANIGUCHI YUICHI 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (90556276)
ISHIDA MAYU 国立研究開発法人理化学研究所, 生命システム研究センター, テクニカルスタッフ
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 蛍光イメージング / 光シート顕微鏡 / 1分子計測 / １分子計測 / 生物物理 |
|---|
| Outline of Final Research Achievements |
We developed a new light sheet microscope and measurement application of a single molecule imaging in a eukaryotic cell. As our light sheet microscope, it was able to measure the single fluorescent molecule in whole body of Mammalian cell. And we demonstrated real time imaging for simultaneous measurement of mRNA and protein expression in a yeast cell. In the future, it is expected to be applied for various cells. We will improve our microscope for high throughput applications as like Drug screening and library analysis.
|
Report
(4 results)
Research Products
(1 results)
