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On Chip Detection of Tau Protein Using Microtubule Based Bioassay

Research Project

Project/Area Number 26790030
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nano/Microsystems
Research InstitutionThe University of Tokyo

Principal Investigator

Tarhan Mehmet Cagatay  東京大学, 生産技術研究所, 研究員 (50582839)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
KeywordsProtein detection / On-chip detection / Tau protein / Microtubules / Kinesin / Molecular detection
Outline of Final Research Achievements

This study shows that development novel tau detection method in an on-chip format. The proposed detection system is based on kinesin-coated bead motion along immobilized MTs. Chemically stabilized MTs (without tau) are used as the rail for kinesin motion. Kinesin molecules work collectively to move a bead along MTs. Kinesin molecule encountering a tau protein on the MT surface stops/detaches. Motion continues when another kinesin molecule starts moving. Such “switching” decreases bead velocity. Results could be achieved with the chip in a time and cost efficient way due to parallel processing. Moreover, a demonstration of an additional pre-incubation technique was introduced, which can solve the existing issues on detecting mutated tau. This novel technique can be used to amplify the sensitivity reaching the physiological levels. After necessary optimizations, clinical samples should be used to test the performance for demonstrating the diagnostic capabilities.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (6 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] On-chip microtubule gliding assay for parallel measurement of tau protein species2016

    • Author(s)
      S. P. Subramaniyan, M. C. Tarhan, S. L. Karsten, H. Fujita, H. Shintaku, H. Kotera and R. Yokokawa
    • Journal Title

      Lab On Chip

      Volume: next volume Issue: 9 Pages: 1691-1697

    • DOI

      10.1039/c5lc01486g

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Nano systems and devices for applications in biology and nanotechnology2016

    • Author(s)
      Perret, G., P. Ginet, M. C. Tarhan, A. Baccouche, T. Lacornerie, M. Kumemura, L. Jalabert et al.
    • Journal Title

      Solid-State Electronics

      Volume: 115 Pages: 66-73

    • DOI

      10.1016/j.sse.2015.08.019

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Point-of-Care (POC) Devices by Means of Advanced MEMS2015

    • Author(s)
      S. L. Karsten, M. C. Tarhan, L. C. Kudo, D. Collard and H. Fujita
    • Journal Title

      Talanta

      Volume: 145 Pages: 55-59

    • DOI

      10.1016/j.talanta.2015.04.032

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Point-of-Care Devices (POCDs) by Means of Advanced MEMS2015

    • Author(s)
      S. L. Karsten, M. C. Tarhan, L. C. Kudo, D. Collard and H. Fujita
    • Journal Title

      Talanta

      Volume: 印刷中

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] DNA mechanical characterization inside biological buffer by the Double-Actuator Silicon Nano Tweezers2016

    • Author(s)
      Gregoire Perret, M. C. Tarhan, Nicolas Lafitte, Laurent Jalabert, M. Kumemura, T. Lacornerie, E. Lartigau, F. Cleri, H. Fujita and D. Collard
    • Organizer
      Asia- Pacific Conference on Transducers and Micro-Nano Technology
    • Place of Presentation
      Kanazawa bunka hall, Kanazawa, Japan
    • Year and Date
      2016-06-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Manipulating Different Biological Samples by Silicon Nanotweezers2016

    • Author(s)
      M. C. Tarhan, G. Perret, N. Lafitte, M. Kumemura, L. Jalabert, Y. Takayama, S. L. Karsten, H. Fujita and D. Collard
    • Organizer
      International Conference on Microtechnologies in Medicine and Biology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2016-04-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3D Humidity Imager in Micro Environment Based on DNA Conductivity and Rigidity Measured by Silicon Nano Tweezers2016

    • Author(s)
      G. Perret, N. Lafitte, M. C. Tarhan, L. Jalabert, M. Kumemura, T. Lacornerie, E. Lartigau, F. Cleri, H. Fujita and D. Collard
    • Organizer
      IEEE International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Shanghai, China
    • Year and Date
      2016-01-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SNTs Combined to a Microfluidic Device for Monitoring the Mechanical Effects of Metal Cations on DNA2015

    • Author(s)
      Y. Tauran, M.C. Tarhan, N. Lafitte, L. Jalabert, B.J. Kim, H. Fujita, A.W. Coleman and D. Collard
    • Organizer
      International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Gyeongju, Korea
    • Year and Date
      2015-10-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] On-Chip Detection of Wild 3R, 4R and Mutant 4R Tau Through Kinesin-Microtubule Binding2015

    • Author(s)
      S. P. Subramaniyan, M. C. Tarhan, S. L. Karsten, H. Fujita, H. Shintaku, H. Kotera and R. Yokokawa
    • Organizer
      IEEE International Conference on Micro Electro Mechanical Systems
    • Place of Presentation
      Estoril, Portugal
    • Year and Date
      2015-01-18 – 2015-01-22
    • Related Report
      2014 Research-status Report
  • [Presentation] Kinesin-microtubule molecular system in microchip format for detection of tau isoforms and mutants2014

    • Author(s)
      S. P. Subramaniyan, M. C. Tarhan, S. L. Karsten, H. Fujita, H. Shintaku, H. Kotera and R. Yokokawa
    • Organizer
      44th annual meeting of the Society for Neuroscience
    • Place of Presentation
      Washington, DC, USA
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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