Robust mechanism of cellular information processing explored from fluctuation of cell movement

• Project/Area Number: 26800226
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Nara Medical University
• Principal Investigator: TAKAGI Hiroaki 奈良県立医科大学, 医学部, 講師 (10444514)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 細胞運動 / 自発性 / 細胞走性 / 時系列解析 / 数理モデル / 細胞性粘菌 / ランジュバン方程式 / T細胞 / インフルエンザウィルス / 神経細胞

Outline of Final Research Achievements

We carried out basic researches to capture the mechanism of information processing of cells from the viewpoint of spontaneous movement (random movement without external stimulus) and fluctuation of cells. We performed comprehensive statistical analysis of spontaneous movement not only in wild-type and mutants of Dictyostelium cells, but also in wild-type mouse T cells and nerve cells. We quantitatively confirmed the common aspects in the statistical features and the applicable mathematical model. Based on the general results obtained there, we proposed a new scheme that spontaneous movement and fluctuation of cells play an active role in producing cellular flexible directional movement (the output of cellular information processing) against external stimulus and its change.

Research Products

• [Journal Article] Unique directional motility of influenza C virus controlled by its filamentous morphology and short-range motions (2018)
  • Author(s): Tatsuya Sakai, Hiroaki Takagi, Yasushi Muraki and Mineki Saito
  • Journal Title: Journal of Virology Volume: 92(2) Issue: 2
  • DOI: 10.1128/jvi.01522-17
  • Peer Reviewed / Open Access
• [Presentation] 異なる細胞種間での自発運動の比較解析 (2017)
  • Author(s): 高木拓明
  • Organizer: 定量生物学の会第八回年会
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市明大寺町）
  • Year and Date: 2017-01-08
• [Presentation] Spontaneous cell migration dynamics in 2D and 3D environment (2016)
  • Author(s): 高木拓明
  • Organizer: 第５４回日本生物物理学会年会
  • Place of Presentation: つくば国際会議場（茨城県つくば市竹園）
  • Year and Date: 2016-11-27
• [Presentation] Spontaneous cell migration dynamics, in different species, in different dimensions (2016)
  • Author(s): Hiroaki Takagi
  • Organizer: NIG International Symposium -Japan Q-bio Week-
  • Place of Presentation: 東京大学生産技術研究所（東京都目黒区駒場）
  • Year and Date: 2016-01-09
• [Presentation] Common aspects of spontaneous cell migration dynamics in different species (2015)
  • Author(s): 高木拓明
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学角間キャンパス（石川県金沢市角間町）
  • Year and Date: 2015-09-13
• [Presentation] 免疫細胞の自発運動の解析 －細胞性粘菌との比較を通して― (2015)
  • Author(s): 高木拓明
  • Organizer: 細胞システムの動態と論理Ⅶ
  • Place of Presentation: 理化学研究所生物科学研究棟（埼玉県和光市広沢）
  • Year and Date: 2015-04-03
• [Presentation] 細胞の自発的運動に見られる種間共通性と差異 (2015)
  • Author(s): 高木拓明
  • Organizer: 第７回定量生物学の会年会
  • Place of Presentation: 九州大学筑紫ホール（福岡県春日市春日公園）
  • Year and Date: 2015-01-11 – 2015-01-12
• [Presentation] T細胞の自発運動の解析 (2014)
  • Author(s): 高木拓明、片貝智哉、木梨達雄
  • Organizer: 第５２回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2014-09-26
• [Presentation] Spontaneous cell migration and its relevance to cellular stochastic sensing mechanism (2014)
  • Author(s): Hiroaki Takagi
  • Organizer: The joint annual meeting of the Japanese society for mathematical biology and the society for mathematical biology
  • Place of Presentation: 大阪国際会議場（大阪府大阪市北区）
  • Year and Date: 2014-07-29