Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Outline of Final Research Achievements |
We have investigated simple method to realize dissolution and refolding of aggregated recombinant protein from E. coli in hydrated ionic liquids. Among the investigated ionic structures, a pair of cholinium cation and dihydrogen phosphate anion showed direct dissolution of the aggregated protein with subsequent formation to the folding state, when it was controlled the number of water molecules.
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