Renaturation of Aggregated Proteins Treated with Hydrated Ionic Liquids
| Project/Area Number |
26810066
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Polymer chemistry
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
FUJITA Kyoko 東京農工大学, 工学(系)研究科(研究院), 講師 (90447508)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | リフォールディング / イオン液体 / 水和 / タンパク質 / 水和状体 / 水和状態 / 凝集体 / 再生 |
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| Outline of Final Research Achievements |
We have investigated simple method to realize dissolution and refolding of aggregated recombinant protein from E. coli in hydrated ionic liquids. Among the investigated ionic structures, a pair of cholinium cation and dihydrogen phosphate anion showed direct dissolution of the aggregated protein with subsequent formation to the folding state, when it was controlled the number of water molecules.
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Report
(3 results)
Research Products
(26 results)
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[Book] Analytical Applications of Ionic Liquids2016
Author(s)
M. Koel, C. Chiappe, K. Fujita, J. Gebicki, E. Lust, C. Zhao, A. Mele, A. Mudring, J. Namiesnik, S. Dai, M. Freire, J. Brenneke, M.Vaher, H. Ohno, V. Pino
Publisher
World scientific Publishing UK Ltd
Related Report
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