Renaturation of Aggregated Proteins Treated with Hydrated Ionic Liquids

• Project/Area Number: 26810066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Polymer chemistry
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: FUJITA Kyoko 東京農工大学, 工学(系)研究科(研究院), 講師 (90447508)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リフォールディング / イオン液体 / 水和 / タンパク質 / 水和状体 / 水和状態 / 凝集体 / 再生

Outline of Final Research Achievements

We have investigated simple method to realize dissolution and refolding of aggregated recombinant protein from E. coli in hydrated ionic liquids. Among the investigated ionic structures, a pair of cholinium cation and dihydrogen phosphate anion showed direct dissolution of the aggregated protein with subsequent formation to the folding state, when it was controlled the number of water molecules.

Research Products

• [Journal Article] Fast electron transfer reaction of azurin fixed on the modified electrode in hydrated ionic liquids (2016)
  • Author(s): K. Fujita, J. Kuwahara, N. Nakamura, H. Ohno
  • Journal Title: Journal of The Electrochemical Society Volume: 163 Issue: 7 Pages: G79-G81
  • DOI: 10.1149/2.0301607jes
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Sugar chain-binding specificity and native folding state of lectin preserved in hydrated ionic liquids (2015)
  • Author(s): K. Fujita, M. Sanada, H. Ohno
  • Journal Title: Chem. Commun. Volume: 51 Issue: 54 Pages: 10883-10886
  • DOI: 10.1039/c5cc03142g
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Is seven the minimum number of water molecules per ion pair for assured biological activity in ionic liquid/water mixtures? (2015)
  • Author(s): Hiroyuki Ohno, Kyoko Fujita, Yuki Kohno
  • Journal Title: Physical Chemistry Chemical Physics Volume: 印刷中 Issue: 22 Pages: 14454-14460
  • DOI: 10.1039/c5cp00768b
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A simple recovery process for biodegradable plastics accumulated in cyanobacteria treated with ionic liquids (2015)
  • Author(s): D. Kobayashi, K. Fujita, N. Nakamura, H. Ohno
  • Journal Title: Appl Microbiol Biotechnol Volume: 99 Issue: 4 Pages: 1647-1653
  • DOI: 10.1007/s00253-014-6234-1
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 水和イオン液体/可溶化剤混合系を用いたコンカナバリンA凝集体の再生 (2016)
  • Author(s): 吉岡大輔・藤田恭子・大野弘幸
  • Organizer: 第65回高分子学会年次大会
  • Place of Presentation: 神戸国際会議場、神戸市、神戸
  • Year and Date: 2016-05-25
• [Presentation] Hydrated Ionic Liquids as Effective Solvent of Renaturation of Aggregated Recombinant Cellulase Expressed in Escherichia Coli (2015)
  • Author(s): Mayuko Kajiyama, Kyoko Fujita, Hiroyuki Ohno
  • Organizer: The International chemical congress of pacific basin societies 2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Improvement of Sugar Chain Recognition of Lectin in Hydrated Ionic Liquid (2015)
  • Author(s): Miki Sanada, Kyoko Fujita, Hiroyuki Ohno,
  • Organizer: The International chemical congress of pacific basin societies 2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Control of redox state of cytochrome c to regulate distribution behaviour in ionic liquids/ buffer system (2015)
  • Author(s): Kazuma Ikeda, Kyoko Fujita, Hiroyuki Ohno Nobuhumi Nakamura
  • Organizer: The International chemical congress of pacific basin societies 2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Effectiveness of Ionic Liquids for Recovery Process of Useful Substrates Accumulated in Cyanobacteria (2015)
  • Author(s): Kyoko Fujita, Daigo Kobayashi, Nobuhumi Nakamura, Hiroyuki Ohno
  • Organizer: The International chemical congress of pacific basin societies 2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Characterization of aptamer forming G-quadruplex structure and its binding to target molecules in hydrated ionic liquid (2015)
  • Author(s): Takuya Honda, Koichi Abe, Kyoko Fujita, Hiroyuki Ohno, Kazunori Ikebukuro
  • Organizer: The International chemical congress of pacific basin societies 2015
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 水和イオン液体を用いたレクチンの保存とリフォールディング挙動の検討 (2015)
  • Author(s): 真田美希、藤田恭子、大野弘幸
  • Organizer: 第24回ポリマー材料フォーラム
  • Place of Presentation: タワーホール船堀、江戸川区、東京都
  • Year and Date: 2015-11-26
• [Presentation] 水和コリニウム系イオン液体中に溶解したコンカナバリンAの糖鎖結合能の検討 (2015)
  • Author(s): 真田美希 藤田恭子 大野弘幸
  • Organizer: 第6回イオン液体討論会
  • Place of Presentation: 同志社大学寒梅館、京都市、京都
  • Year and Date: 2015-10-26
• [Presentation] LCST型挙動を示すイオン液体/緩衝液二相系でのシトクロムcの電気化学的な分配制御 (2015)
  • Author(s): 池田一磨、藤田恭子、大野弘幸、中村暢文
  • Organizer: 第6回イオン液体討論会
  • Place of Presentation: 同志社大学寒梅館、京都市、京都
  • Year and Date: 2015-10-26
• [Presentation] Recovery process for materials accumulated in cyanobacteria treated with ionic liquids (2015)
  • Author(s): Kyoko Fujita
  • Organizer: CyanoFactory Meeting Bochum 2015
  • Place of Presentation: Bochum, Germany
  • Year and Date: 2015-10-01
  • Int'l Joint Research / Invited
• [Presentation] Preservation of Lectin in Hydrated Ionic Liquid (2015)
  • Author(s): Miki Sanada, Kyoko Fujita, Hiroyuki Ohno
  • Organizer: 5th International Congress on Ionic Liquids
  • Place of Presentation: Jeju, Koria
  • Year and Date: 2015-06-17
  • Int'l Joint Research
• [Presentation] Renaturation of Aggregated Recombinant Cellulase Expressed in Escherichia Coli by Dissolution in Hydrated Ionic Liquids (2015)
  • Author(s): Mayuko Kajiyama, Kyoko Fujita, Hiroyuki Ohno
  • Organizer: 5th International Congress on Ionic Liquids
  • Place of Presentation: Jeju, Koria
  • Year and Date: 2015-06-17
  • Int'l Joint Research
• [Presentation] Recovery Process for Biodegradable Plastics Accumulated in Cyanobacteria Treated with Ionic Liquids (2015)
  • Author(s): Kyoko Fujita, Daigo Kobayashi, Nobuhumi Nakamura, Hiroyuki Ohno
  • Organizer: 5th International Congress on Ionic Liquids
  • Place of Presentation: Jeju, Koria
  • Year and Date: 2015-06-17
  • Int'l Joint Research
• [Presentation] イオン液体/緩衝液二相系におけるトリエチレングリコール修飾シトクロムc の分配挙動の解析 (2015)
  • Author(s): 池田 一磨、藤田 恭子、中村 暢文、大野 弘幸
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学、船橋市
  • Year and Date: 2015-03-28
• [Presentation] Concanavalin Aの水和イオン液体への溶解と安定性への影響 (2015)
  • Author(s): 真田 美希、藤田 恭子、大野 弘幸
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 日本大学、船橋市
  • Year and Date: 2015-03-28
• [Presentation] Evaluation of Folding State of Concanavalin A Dissolved in Hydrated Cholinium Dihydrogen Phosphate (2014)
  • Author(s): K. Fujita
  • Organizer: 2014 Sophia Symposium on Ionic Liquids
  • Place of Presentation: 上智大学、東京都
  • Year and Date: 2014-10-31
  • Invited
• [Presentation] 水和コリニウムリン酸二水素に溶解したコンカナバリンAのフォールディング状態の解析 (2014)
  • Author(s): 藤田 恭子, 真田 美希, 大野 弘幸
  • Organizer: 第5回イオン液体討論会
  • Place of Presentation: 横浜シンポジア、横浜市
  • Year and Date: 2014-10-29
• [Presentation] Thermal behavior of Concanavalin A Dissolved in Hydrated Cholinium Dihydrogen Phosphate (2014)
  • Author(s): K. Fujita, A. Takasaki, Y. Fujita-Yamaguchi, H. Ohno
  • Organizer: 4th Asia-pacific conference on ionic liquids and green process/ 6th Australasian symposium on ionic liquid 2014
  • Place of Presentation: Australia, sydney
  • Year and Date: 2014-10-01
• [Presentation] 水和コリニウムリン酸二水素中に溶解したコンカナバリンAのフォールディング状態 (2014)
  • Author(s): 藤田 恭子、大野 弘幸
  • Organizer: 第63回高分子討論会
  • Place of Presentation: 長崎大学、長崎市
  • Year and Date: 2014-09-26
• [Presentation] 水和イオン液体を用いた大腸菌内で凝集したセルラーゼの再生 (2014)
  • Author(s): 梶山 万悠子、藤田 恭子、大野 弘幸
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 岡山大学、岡山市
  • Year and Date: 2014-09-11
• [Book] Analytical Applications of Ionic Liquids (2016)
  • Author(s): M. Koel, C. Chiappe, K. Fujita, J. Gebicki, E. Lust, C. Zhao, A. Mele, A. Mudring, J. Namiesnik, S. Dai, M. Freire, J. Brenneke, M.Vaher, H. Ohno, V. Pino
  • Publisher: World scientific Publishing UK Ltd
• [Remarks] 研究内容（大野研究室HP内）
  • URL: http://www.tuat.ac.jp/~ohno/research/research.html