Development of sensor arrays consisting of polyion complexes between enzymes and polyelectrolytes for markerless and noninvasive evaluation of stem cells

• Project/Area Number: 26810074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Tomita Shunsuke 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (50726817)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 幹細胞 / 酵素 / 高分子電解質 / 多変量解析 / バイオセンサ / ポリイオン複合体

Outline of Final Research Achievements

We developed a library of polyion complexes between enzymes and polyelectrolytes for markerless and noninvasive evaluation of stem cells. Cationic block copolymers with various hydrophobic groups were first synthesized, and used to construct highly cross-reactive molecular libraries by the formation of complexes with anionic enzymes. Analysis of cell culture supernatants using these libraries allowed not only discrimination of different cancer cell lines but also lineage identification of osteogenic and adipogenic differentiation of human mesenchymal stem cells.

Research Products

• [Journal Article] Wrap-and-Strip technology of protein; polyelectrolyte complex for biomedical application (2016)
  • Author(s): Kentaro Shiraki, Takaaki Kurinomaru, Shunsuke Tomita
  • Journal Title: Current Medicinal Chemistry Volume: 23 Issue: 3 Pages: 276-289
  • DOI: 10.2174/0929867323666151127201126
  • NAID: 120007135563
  • Peer Reviewed
• [Journal Article] Small amine molecules: Solvent design toward facile improvement of protein stability against aggregation and inactivation (2016)
  • Author(s): Kentaro Shiraki, Shunsuke Tomita, Naoto Inoue
  • Journal Title: Current Pharmaceutical Biotechnology Volume: 17 Issue: 2 Pages: 116-125
  • DOI: 10.2174/1389201017666151029110229
  • NAID: 120007135454
  • Peer Reviewed / Open Access
• [Journal Article] The Use of an Enzyme-based Sensor Array to Fingerprint Proteomic Signatures of Sera from Different Mammalian Species (2016)
  • Author(s): Shunsuke Tomita*, Saki Yokoyama, Ryoji Kurita, Osamu Niwa, Keitaro Yoshimoto*
  • Journal Title: Analytical Sciences Volume: 32 Issue: 2 Pages: 237-240
  • DOI: 10.2116/analsci.32.237
  • NAID: 130005125633
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Polyion Complex Sensor Array for Markerless and Noninvasive Identification of Differentiated Mesenchymal Stem Cells from Human Adipose Tissue (2015)
  • Author(s): Shunsuke Tomita*, Miho Sakao, Ryoji Kurita, Osamu Niwa, Keitaro Yoshimoto*
  • Journal Title: Chemical Science Volume: 6 Issue: 10 Pages: 5831-5836
  • DOI: 10.1039/c5sc01259g
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Enzymatic fingerprinting of structurally similar homologous proteins using polyion complex library constructed by tuning PEGylated polyamine functionalitiesion (2014)
  • Author(s): Tomita, S., Soejima, T., Shiraki, K., Yoshimoto, K.
  • Journal Title: Analyst Volume: 139(23) Issue: 23 Pages: 6100-6103
  • DOI: 10.1039/c4an01398k
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Charge-Separated Fmoc-peptide β-Sheets: Sequence-Secondary Structure Relationship for Arranging Charged Side Chains on Both Sides (2014)
  • Author(s): Toru Nakayama,Taro Sakuraba, Shunsuke Tomita, Akira Kaneko, Eisuke Takai, Kentaro Shiraki, Kentaro Tashiro, Noriyuki Ishii, Yuri Hasegawa, Yoichi Yamada, Reiji Kumai, and Yohei Yamamoto
  • Journal Title: Asian J. Org. Chem. Volume: 3 Issue: 11 Pages: 1182-1182
  • DOI: 10.1002/ajoc.201402129
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Design of polyion complex sensor arrays that fingerprint secretomic signatures of cells for markerless and noninvasive cell identification (2015)
  • Author(s): Shunsuke Tomita, Keitaro Yoshimoto, Ryoji Kurita, Osamu Niwa
  • Organizer: Pacifichem2015
  • Place of Presentation: Marriott Waikiki Beach (United States)
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Enzyme-incorporated polyion complexes as novel materials for secretome fingerprinting of differentiated mesenchymal stem cells. (2015)
  • Author(s): Shunsuke Tomita, Osamu Niwa, Ryoji Kurita, Keitaro Yoshimoto
  • Organizer: 第25回日本MRS年次大会
  • Place of Presentation: 横浜市開港記念会館（神奈川県）
  • Year and Date: 2015-12-08
• [Presentation] ポリイオン複合体ライブラリを用いる 分化間葉系幹細胞セクレトームのフィンガープリンティング" (2015)
  • Author(s): 冨田 峻介、丹羽 修、栗田 僚二、吉本 敬太郎
  • Organizer: 第３０回 高分子学会関東支部茨城地区 若手の会交流会
  • Place of Presentation: つくばセミナーハウス（茨城県）
  • Year and Date: 2015-10-29
• [Presentation] 酵素のその場修飾を利用する簡易なポリイオン複合体ライブラリ作製法の開発とアレイ型バイオセンシングへの応用 (2015)
  • Author(s): 冨田 峻介、丹羽 修、栗田 僚二
  • Organizer: 日本分析化学会第64年会
  • Place of Presentation: 九州大学伊都キャンパス（福岡県）
  • Year and Date: 2015-09-09
• [Presentation] Toward facile, markerless, and noninvasive determination of stem cell states: Pattern recognition-based sensing of proteins secreted from differentiated human mesenchymal stem cells " (2015)
  • Author(s): Shunsuke Tomita, Osamu Niwa, Ryoji Kurita, Keitaro Yoshimoto
  • Organizer: International Symposium on Nanoarchitectonics for Mechanobiology (ISNM) "
  • Place of Presentation: WPI-MANA Auditorium, NIMS (Ibaraki-ken)
  • Year and Date: 2015-07-29
  • Int'l Joint Research
• [Presentation] 味覚に学ぶバイオ分析： マーカー分子に頼らない生体試料センシングを目指して" (2015)
  • Author(s): 冨田 峻介
  • Organizer: 平成 27 年度日本分析化学会関東支部若手交流会
  • Place of Presentation: 晴海グランドホテル（東京都）
  • Year and Date: 2015-06-26
  • Invited
• [Presentation] ポリイオン複合体ライブラリを用いる味認識を模倣した生体サンプルセンシング (2014)
  • Author(s): 冨田峻介, 横山沙樹, 坂尾美帆, 副島智大, 吉本敬太郎
  • Organizer: 第29回高分子学会関東支部茨城地区若手の会交流会
  • Place of Presentation: つくばセミナーハウス
  • Year and Date: 2014-10-30
• [Presentation] 味認識を模倣した非特異的センシングによる生体サンプル判別法の開発 (2014)
  • Author(s): 冨田峻介, 横山沙樹, 坂尾美帆, 副島智大, 丹羽修, 吉本敬太郎
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 広島大学東広島キャンパス
  • Year and Date: 2014-09-18
• [Remarks] 個人ホームページ
  • URL: https://staff.aist.go.jp/s.tomita/