Development of sensor arrays consisting of polyion complexes between enzymes and polyelectrolytes for markerless and noninvasive evaluation of stem cells
| Project/Area Number |
26810074
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Tomita Shunsuke 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (50726817)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 幹細胞 / 酵素 / 高分子電解質 / 多変量解析 / バイオセンサ / ポリイオン複合体 |
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| Outline of Final Research Achievements |
We developed a library of polyion complexes between enzymes and polyelectrolytes for markerless and noninvasive evaluation of stem cells. Cationic block copolymers with various hydrophobic groups were first synthesized, and used to construct highly cross-reactive molecular libraries by the formation of complexes with anionic enzymes. Analysis of cell culture supernatants using these libraries allowed not only discrimination of different cancer cell lines but also lineage identification of osteogenic and adipogenic differentiation of human mesenchymal stem cells.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Charge-Separated Fmoc-peptide β-Sheets: Sequence-Secondary Structure Relationship for Arranging Charged Side Chains on Both Sides2014
Author(s)
Toru Nakayama,Taro Sakuraba, Shunsuke Tomita, Akira Kaneko, Eisuke Takai, Kentaro Shiraki, Kentaro Tashiro, Noriyuki Ishii, Yuri Hasegawa, Yoichi Yamada, Reiji Kumai, and Yohei Yamamoto
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Journal Title
Asian J. Org. Chem.
Volume: 3
Issue: 11
Pages: 1182-1182
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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