| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Outline of Final Research Achievements |
As a fundamental study to develop a new peripheral blood circulating tumor cell (CTC) detection system based on the information of intracellular elemental contents, a highly efficient cell separation technique using a microfluidic device and a highly sensitive distribution measurement method of specific marker element contents in various types of cells using single-cell mass spectrometry were examined. Different types of microfluidic devices for cell separation were experimentally produced and evaluated, and consequently a pinched flow chip device turned out to have higher efficient and better separation capabilities than others. The component technologies of single-cell mass spectrometry were examined, and it was demonstrated that a direct cell introduction interface is ideal for sample introduction due to its high transport efficiency of cells into the ion source of mass spectrometer, and that copper or zinc is useful as a specific marker element for identifying CTC.
|
