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Development of a peripheral blood circulating tumor cell (CTC) detection system based on the information of intracellular elemental contents

Research Project

Project/Area Number 26810082
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

MIYASHITA Shin-ichi  国立研究開発法人産業技術総合研究所, 物質計測標準研究部門, 主任研究員 (60614766)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsマイクロ流路分析 / がん診断 / 血球 / 循環腫瘍細胞 / 質量分析
Outline of Final Research Achievements

As a fundamental study to develop a new peripheral blood circulating tumor cell (CTC) detection system based on the information of intracellular elemental contents, a highly efficient cell separation technique using a microfluidic device and a highly sensitive distribution measurement method of specific marker element contents in various types of cells using single-cell mass spectrometry were examined. Different types of microfluidic devices for cell separation were experimentally produced and evaluated, and consequently a pinched flow chip device turned out to have higher efficient and better separation capabilities than others. The component technologies of single-cell mass spectrometry were examined, and it was demonstrated that a direct cell introduction interface is ideal for sample introduction due to its high transport efficiency of cells into the ion source of mass spectrometer, and that copper or zinc is useful as a specific marker element for identifying CTC.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] 高効率試料導入システムを用いた高時間分解ICP-MSによる単一ナノ粒子・細胞分析2017

    • Author(s)
      宮下振一、藤井紳一郎、稲垣和三
    • Journal Title

      分析化学

      Volume: 印刷中

    • NAID

      130006135725

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] シングルパーティクル ICP-MS: ナノ粒子分析法としてのtime-resolved ICP-MS2017

    • Author(s)
      稲垣和三、宮下振一、藤井紳一郎、藤本俊幸
    • Journal Title

      ぶんせき

      Volume: 2017 Pages: 105-111

    • Related Report
      2016 Annual Research Report
  • [Journal Article] High transport efficiency of nanoparticles through a total-consumption sample introduction system and its beneficial application for particle size evaluation in single-particle ICP-MS2016

    • Author(s)
      宮下振一、三橋弘明、藤井紳一郎、高津章子、稲垣和三、藤本俊幸
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 409 Issue: 6 Pages: 1531-1545

    • DOI

      10.1007/s00216-016-0089-5

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] シングルパーティクルICP-MSにおけるナノ粒子のプラズマ導入効率の評価2015

    • Author(s)
      宮下振一、三橋弘明、藤井紳一郎、日置昭治、高津章子、藤本俊幸、稲垣和三
    • Journal Title

      日本分析化学会第64年会「展望とトピックス」

      Volume: - Pages: 20-20

    • Related Report
      2015 Research-status Report
    • Open Access
  • [Presentation] Characterization of nanoparticles uptake and adsorption by red microalgal cells using single particle ICP-MS2017

    • Author(s)
      宮下振一、三橋弘明、藤井紳一郎、安保充、高津章子、稲垣和三
    • Organizer
      2017 European Winter Conference on Plasma Spectrochemestry
    • Place of Presentation
      ARLBERG-well.com Convention Center、サンクト・アントン・アールベルク(オーストリア)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of Transport Efficiency of Nanoparticles Through a Total Consumption Sample Introduction System in Single Particle ICP-MS2016

    • Author(s)
      宮下振一、三橋弘明、藤井紳一郎、高津章子、稲垣和三
    • Organizer
      2016 Winter Conference on Plasma Spectrochemistry
    • Place of Presentation
      Hilton Tucson El Conquistador Golf & Tennis Resort、ツーソン、アメリカ
    • Year and Date
      2016-01-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] High Performance Concentric Nebulizer for ICP-MS: It’s Application for Single-cells and Nanoparticle Analysis2016

    • Author(s)
      宮下振一、藤井紳一郎、稲垣和三
    • Organizer
      2016 Korean Society for Mass Spectrometry Summer Conference
    • Place of Presentation
      Changwon Convention Center、慶州(韓国)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 時間分解ICP-MSを用いた微細藻類と金属ナノ粒子の相互作用解析2016

    • Author(s)
      宮下振一、三橋弘明、藤井紳一郎、安保充、高津章子、稲垣和三
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学(北海道・札幌市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] シングルパーティクルICP-MSにおけるナノ粒子のプラズマ導入効率の評価2015

    • Author(s)
      宮下振一、三橋弘明、藤井紳一郎、日置昭治、高津章子、藤本俊幸、稲垣和三
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      九州大学(福岡)
    • Year and Date
      2015-09-08
    • Related Report
      2015 Research-status Report
  • [Book] Metallomics - Recent Analytical Techniques and Selected Applications -2017

    • Author(s)
      宮下振一、藤井紳一郎、重田香織、稲垣和三
    • Total Pages
      364
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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